HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 37, 38360-38368, September 10, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/37/38360    most recent M405485200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nagawa, F. Articles by Sakano, H. Search for Related Content PubMed PubMed Citation Articles by Nagawa, F. Articles by Sakano, H. Social Bookmarking                      What's this?

Joining Mutants of RAG1 and RAG2 that Demonstrate Impaired Interactions with the Coding-end DNA^*

Fumikiyo Nagawa, Satoshi Hirose, Hirofumi Nishizumi, Tadashi Nishihara, and Hitoshi Sakano

From the Department of Biophysics and Biochemistry, Graduate School of Science, and Core Research for Evolutional Science and Technology Program of the Japan Science and Technology Agency, The University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan

In V(D)J joining of antigen receptor genes, two recombination signal sequences (RSSs), 12- and 23-RSSs, form a complex with the protein products of recombination activating genes, RAG1 and RAG2. DNaseI footprinting demonstrates that the interaction of RAG proteins with substrate RSS DNA is not just limited to the signal region but involves the coding sequence as well. Joining mutants of RAG1 and RAG2 demonstrate impaired interactions with the coding region in both pre- and postcleavage type complexes. A possible role of this RAG coding region interaction is discussed in the context of V(D)J recombination.

Received for publication, May 17, 2004 , and in revised form, June 16, 2004.

^* This work was supported in part by grants from the Ministry of Education Culture and Science of Japan and the Japan Science and Technology Agency. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Both authors contributed equally to this work.

To whom correspondence should be addressed. Tel.: 81-3-5841-7239; Fax: 81-3-5841-7240; E-mail: sakano{at}mail.ecc.u-tokyo.ac.jp.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Kumar and P. C. Swanson Full-length RAG1 promotes contact with coding and intersignal sequences in RAG protein complexes bound to recombination signals paired in cis Nucleic Acids Res., April 1, 2009; 37(7): 2211 - 2226. [Abstract] [Full Text] [PDF]

				W. Giblin, M. Chatterji, G. Westfield, T. Masud, B. Theisen, H.-L. Cheng, J. DeVido, F. W. Alt, D. O. Ferguson, D. G. Schatz, et al. Leaky severe combined immunodeficiency and aberrant DNA rearrangements due to a hypomorphic RAG1 mutation Blood, March 26, 2009; 113(13): 2965 - 2975. [Abstract] [Full Text] [PDF]

				T. Nishihara, F. Nagawa, T. Imai, and H. Sakano RAG-Heptamer Interaction in the Synaptic Complex Is a Crucial Biochemical Checkpoint for the 12/23 Recombination Rule J. Biol. Chem., February 22, 2008; 283(8): 4877 - 4885. [Abstract] [Full Text] [PDF]

				A. H. Drejer-Teel, S. D. Fugmann, and D. G. Schatz The Beyond 12/23 Restriction Is Imposed at the Nicking and Pairing Steps of DNA Cleavage during V(D)J Recombination Mol. Cell. Biol., September 15, 2007; 27(18): 6288 - 6299. [Abstract] [Full Text] [PDF]

				M. Ciubotaru, A. N. Kriatchko, P. C. Swanson, F. V. Bright, and D. G. Schatz Fluorescence Resonance Energy Transfer Analysis of Recombination Signal Sequence Configuration in the RAG1/2 Synaptic Complex Mol. Cell. Biol., July 1, 2007; 27(13): 4745 - 4758. [Abstract] [Full Text] [PDF]

				M. Larijani, A. Zaheen, D. Frieder, Y. Wang, G. E. Wu, W. Edelmann, and A. Martin Lack of MSH2 involvement differentiates V(D)J recombination from other non-homologous end joining events Nucleic Acids Res., November 27, 2005; 33(21): 6733 - 6742. [Abstract] [Full Text] [PDF]