HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 37, 39122-39131, September 10, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/37/39122    most recent M401044200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cárdenas, C. Articles by Carrasco, M. A. Search for Related Content PubMed PubMed Citation Articles by Cárdenas, C. Articles by Carrasco, M. A. Social Bookmarking                      What's this?

Depolarization of Skeletal Muscle Cells induces Phosphorylation of cAMP Response Element Binding Protein via Calcium and Protein Kinase C^*

César Cárdenas, Marioly Müller, Enrique Jaimovich, Francisco Pérez, Diego Buchuk, Andrew F. G. Quest, and Maria Angélica Carrasco¶

From the Centro de Estudios Moleculares de la Célula, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Independencia 1027, Santiago 7, Chile

Membrane depolarization of skeletal muscle cells induces slow inositol trisphosphate-mediated calcium signals that regulate the activity of transcription factors such as the cAMP-response element-binding protein (CREB), jun, and fos. Here we investigated whether such signals regulate CREB phosphorylation via protein kinase C (PKC)-dependent pathways. Western blot analysis revealed the presence of seven isoforms (PKC, -I, -II, -, -, -, and -) in rat primary myotubes. The PKC inhibitors bisindolymaleimide I and Gö6976, blocked CREB phosphorylation. Chronic exposure to phorbol ester triggered complete down-regulation of several isoforms, but reduced PKC levels to only 40%, and did not prevent CREB phosphorylation upon myotube depolarization. Immunocytochemical analysis revealed selective and rapid PKC translocation to the nucleus following depolarization, which was blocked by 2-amino-ethoxydiphenyl borate, an inositol trisphosphate receptor inhibitor, and by the phospholipase C inhibitor U73122. In C2C12 cells, which expressed PKC,-, and -, CREB phosphorylation also depended on PKC. These results strongly implicate nuclear PKC translocation in CREB phosphorylation induced by skeletal muscle membrane depolarization.

Received for publication, January 30, 2004 , and in revised form, June 30, 2004.

^* This work was supported by Fondo de Investigación Avanzada en Areas Prioritarias Grant 15010006 and by Fondo Nacional de Desarrollo Científico y Tecnológico Grants 1030988 and 1020585. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Recipient of a graduate student fellowship from the Comisión Nacional de Investigación Científica y Tecnológica and Programa de Mejoramiento de la Calidad y la Equidad de la Educación Superior UCH9903.

To whom correspondence may be addressed. Tel.: 56-2-678-2015; Fax: 56-2-678-2015; E-mail: aquest{at}med.uchile.cl.

^¶ To whom correspondence may be addressed. Tel.: 56-2-678-6312; Fax: 56-2-777-6916; E-mail: mcarras{at}med.uchile.cl.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				N. Ozgen, M. Obreztchikova, J. Guo, H. Elouardighi, G. W. Dorn II, B. A. Wilson, and S. F. Steinberg Protein Kinase D Links Gq-coupled Receptors to cAMP Response Element-binding Protein (CREB)-Ser133 Phosphorylation in the Heart J. Biol. Chem., June 20, 2008; 283(25): 17009 - 17019. [Abstract] [Full Text] [PDF]

				Y. Hu, I. V. Lund, M. C. Gravielle, D. H. Farb, A. R. Brooks-Kayal, and S. J. Russek Surface Expression of GABAA Receptors Is Transcriptionally Controlled by the Interplay of cAMP-response Element-binding Protein and Its Binding Partner Inducible cAMP Early Repressor J. Biol. Chem., April 4, 2008; 283(14): 9328 - 9340. [Abstract] [Full Text] [PDF]

				J. A. Valdes, E. Gaggero, J. Hidalgo, N. Leal, E. Jaimovich, and M. A. Carrasco NFAT activation by membrane potential follows a calcium pathway distinct from other activity-related transcription factors in skeletal muscle cells Am J Physiol Cell Physiol, March 1, 2008; 294(3): C715 - C725. [Abstract] [Full Text] [PDF]

				C. Evangelisti, P. L. Tazzari, M. Riccio, R. Fiume, Y. Hozumi, F. Fala, K. Goto, L. Manzoli, L. Cocco, and A. M. Martelli Nuclear diacylglycerol kinase-{zeta} is a negative regulator of cell cycle progression in C2C12 mouse myoblasts FASEB J, October 1, 2007; 21(12): 3297 - 3307. [Abstract] [Full Text] [PDF]

				J. A. Valdes, J. Hidalgo, J. L. Galaz, N. Puentes, M. Silva, E. Jaimovich, and M. A. Carrasco NF-{kappa}B activation by depolarization of skeletal muscle cells depends on ryanodine and IP3 receptor-mediated calcium signals Am J Physiol Cell Physiol, May 1, 2007; 292(5): C1960 - C1970. [Abstract] [Full Text] [PDF]

				A. M. Hennige, N. Stefan, K. Kapp, R. Lehmann, C. Weigert, A. Beck, K. Moeschel, J. Mushack, E. Schleicher, and H.-U. Haring Leptin down-regulates insulin action through phosphorylation of serine-318 in insulin receptor substrate 1 FASEB J, June 1, 2006; 20(8): 1206 - 1208. [Abstract] [Full Text] [PDF]

				N. Juretic, P. Garcia-Huidobro, J. A. Iturrieta, E. Jaimovich, and N. Riveros Depolarization-induced slow Ca2+ transients stimulate transcription of IL-6 gene in skeletal muscle cells Am J Physiol Cell Physiol, May 1, 2006; 290(5): C1428 - C1436. [Abstract] [Full Text] [PDF]

				J. M. Eltit, A. A. Garcia, J. Hidalgo, J. L. Liberona, M. Chiong, S. Lavandero, E. Maldonado, and E. Jaimovich Membrane Electrical Activity Elicits Inositol 1,4,5-Trisphosphate-dependent Slow Ca2+ Signals through a Gbeta{gamma}/Phosphatidylinositol 3-Kinase {gamma} Pathway in Skeletal Myotubes J. Biol. Chem., April 28, 2006; 281(17): 12143 - 12154. [Abstract] [Full Text] [PDF]

				C. Cardenas, J. L. Liberona, J. Molgo, C. Colasante, G. A. Mignery, and E. Jaimovich Nuclear inositol 1,4,5-trisphosphate receptors regulate local Ca2+ transients and modulate cAMP response element binding protein phosphorylation J. Cell Sci., July 15, 2005; 118(14): 3131 - 3140. [Abstract] [Full Text] [PDF]