Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M402202200 on June 21, 2004

J. Biol. Chem., Vol. 279, Issue 38, 39268-39278, September 17, 2004
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
279/38/39268    most recent
M402202200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sanvicens, N.
Right arrow Articles by Cotter, T. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sanvicens, N.
Right arrow Articles by Cotter, T. G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Oxidative Stress-induced Apoptosis in Retinal Photoreceptor Cells Is Mediated by Calpains and Caspases and Blocked by the Oxygen Radical Scavenger CR-6*

Nuria Sanvicens{ddagger}§, Violeta Gómez-Vicente{ddagger}§, Isabel Masip¶, Angel Messeguer¶, and Thomas G. Cotter{ddagger}||

From the {ddagger}Tumour Biology Laboratory, Department of Biochemistry, Bioscience Research Institute, University College, Cork, Ireland and the Department of Biological Organic Chemistry, Institut d'Investigacions auímiques i Ambientals de Barcelona, Consejo Superior de Investigaciones Científicas, Jordi Girona 18-26, 08034 Barcelona, Spain

A critical role for reactive oxygen species (ROS) in photoreceptor apoptosis has been established. However, the exact molecular mechanisms triggered by oxidative stress in photoreceptor cell death remain undefined. This study delineates the molecular events that occur after treatment of the photoreceptor cell line 661W with the nitric oxide donor sodium nitroprusside (SNP). Cytosolic calcium levels increased during photoreceptor apoptosis, leading to activation of the calcium-dependent proteases calpains. Furthermore, caspase activation also occurred following SNP insult. However, although treatment with the pan-caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp fluoromethyl ketone inhibited caspase activity per se in SNP-treated 661W cells, it did not prevent apoptosis. On the other hand, CR-6 (3,4-dihydro-6-hydroxy-7-methoxy-2,2-dimethyl-1(2H)-benzopyran) acted as a scavenger of ROS and reduced 661W photoreceptor apoptosis induced by SNP by preventing the activation of a pathway in which calpains have a key role. In summary, we report for the first time that both caspases and calpains are involved in 661W photoreceptor apoptosis and that calpain activation can be prevented by the ROS scavenger CR-6.

Received for publication, February 27, 2004 , and in revised form, May 26, 2004.

* This work was supported by the European Union, Fighting Blindness Ireland, and the Higher Education Authority of Ireland. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Both authors contributed equally to this work.

|| To whom correspondence should be addressed. Tel.: 353-21-490-1321; Fax: 353-21-490-1377; E-mail: t.cotter{at}ucc.ie.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 IOVSHome page
Z.-M. Bian, S. G. Elner, and V. M. Elner
Regulated Expression of Caspase-12 Gene in Human Retinal Pigment Epithelial Cells Suggests Its Immunomodulating Role
Invest. Ophthalmol. Vis. Sci., December 1, 2008; 49(12): 5593 - 5601.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. M. McClung, M. A. Whidden, A. N. Kavazis, D. J. Falk, K. C. DeRuisseau, and S. K. Powers
Redox regulation of diaphragm proteolysis during mechanical ventilation
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2008; 294(5): R1608 - R1617.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
M. Zhang, B. Marshall, and S. S. Atherton
Murine Cytomegalovirus Infection and Apoptosis in Organotypic Retinal Cultures
Invest. Ophthalmol. Vis. Sci., January 1, 2008; 49(1): 295 - 303.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
Q. Liu, W.-K. Ju, J. G. Crowston, F. Xie, G. Perry, M. A. Smith, J. D. Lindsey, and R. N. Weinreb
Oxidative Stress Is an Early Event in Hydrostatic Pressure Induced Retinal Ganglion Cell Damage
Invest. Ophthalmol. Vis. Sci., October 1, 2007; 48(10): 4580 - 4589.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
A M Lobascio, F G Klinger, M L Scaldaferri, D Farini, and M De Felici
Analysis of programmed cell death in mouse fetal oocytes
Reproduction, August 1, 2007; 134(2): 241 - 252.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
G. S. Supinski and L. A. Callahan
Free radical-mediated skeletal muscle dysfunction in inflammatory conditions
J Appl Physiol, May 1, 2007; 102(5): 2056 - 2063.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
A. Neekhra, S. Luthra, M. Chwa, G. Seigel, A. L. Gramajo, B. D. Kuppermann, and M. C. Kenney
Caspase-8, -12, and -3 Activation by 7-Ketocholesterol in Retinal Neurosensory Cells
Invest. Ophthalmol. Vis. Sci., March 1, 2007; 48(3): 1362 - 1367.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
G. Li, R. E. Anderson, H. Tomita, R. Adler, X. Liu, D. J. Zack, and R. V. S. Rajala
Nonredundant Role of Akt2 for Neuroprotection of Rod Photoreceptor Cells from Light-Induced Cell Death
J. Neurosci., January 3, 2007; 27(1): 203 - 211.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
Y. Kanan, G. Moiseyev, N. Agarwal, J.-X. Ma, and M. R. Al-Ubaidi
Light Induces Programmed Cell Death by Activating Multiple Independent Proteases in a Cone Photoreceptor Cell Line
Invest. Ophthalmol. Vis. Sci., January 1, 2007; 48(1): 40 - 51.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
M. M. Tiwari, K. J. Messer, and P. R. Mayeux
Inducible Nitric Oxide Synthase and Apoptosis in Murine Proximal Tubule Epithelial Cells
Toxicol. Sci., June 1, 2006; 91(2): 493 - 500.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. Zhou, R. Kohl, B. Herr, R. Frank, and B. Brune
Calpain Mediates a von Hippel-Lindau Protein-independent Destruction of Hypoxia-inducible Factor-1{alpha}
Mol. Biol. Cell, April 1, 2006; 17(4): 1549 - 1558.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
F. Doonan, M. Donovan, and T. G. Cotter
Activation of Multiple Pathways during Photoreceptor Apoptosis in the rd Mouse
Invest. Ophthalmol. Vis. Sci., October 1, 2005; 46(10): 3530 - 3538.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
L. E. Broker, F. A.E. Kruyt, and G. Giaccone
Cell Death Independent of Caspases: A Review
Clin. Cancer Res., May 1, 2005; 11(9): 3155 - 3162.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2004 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement