| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 279, Issue 38, 39268-39278, September 17, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
||
From the
Tumour Biology Laboratory, Department of Biochemistry, Bioscience Research Institute, University College, Cork, Ireland and the ¶Department of Biological Organic Chemistry, Institut d'Investigacions auímiques i Ambientals de Barcelona, Consejo Superior de Investigaciones Científicas, Jordi Girona 18-26, 08034 Barcelona, Spain
A critical role for reactive oxygen species (ROS) in photoreceptor apoptosis has been established. However, the exact molecular mechanisms triggered by oxidative stress in photoreceptor cell death remain undefined. This study delineates the molecular events that occur after treatment of the photoreceptor cell line 661W with the nitric oxide donor sodium nitroprusside (SNP). Cytosolic calcium levels increased during photoreceptor apoptosis, leading to activation of the calcium-dependent proteases calpains. Furthermore, caspase activation also occurred following SNP insult. However, although treatment with the pan-caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp fluoromethyl ketone inhibited caspase activity per se in SNP-treated 661W cells, it did not prevent apoptosis. On the other hand, CR-6 (3,4-dihydro-6-hydroxy-7-methoxy-2,2-dimethyl-1(2H)-benzopyran) acted as a scavenger of ROS and reduced 661W photoreceptor apoptosis induced by SNP by preventing the activation of a pathway in which calpains have a key role. In summary, we report for the first time that both caspases and calpains are involved in 661W photoreceptor apoptosis and that calpain activation can be prevented by the ROS scavenger CR-6.
Received for publication, February 27, 2004 , and in revised form, May 26, 2004.
* This work was supported by the European Union, Fighting Blindness Ireland, and the Higher Education Authority of Ireland. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Both authors contributed equally to this work.
|| To whom correspondence should be addressed. Tel.: 353-21-490-1321; Fax: 353-21-490-1377; E-mail: t.cotter{at}ucc.ie.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Z.-M. Bian, S. G. Elner, and V. M. Elner Regulated Expression of Caspase-12 Gene in Human Retinal Pigment Epithelial Cells Suggests Its Immunomodulating Role Invest. Ophthalmol. Vis. Sci., December 1, 2008; 49(12): 5593 - 5601. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. McClung, M. A. Whidden, A. N. Kavazis, D. J. Falk, K. C. DeRuisseau, and S. K. Powers Redox regulation of diaphragm proteolysis during mechanical ventilation Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2008; 294(5): R1608 - R1617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zhang, B. Marshall, and S. S. Atherton Murine Cytomegalovirus Infection and Apoptosis in Organotypic Retinal Cultures Invest. Ophthalmol. Vis. Sci., January 1, 2008; 49(1): 295 - 303. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Liu, W.-K. Ju, J. G. Crowston, F. Xie, G. Perry, M. A. Smith, J. D. Lindsey, and R. N. Weinreb Oxidative Stress Is an Early Event in Hydrostatic Pressure Induced Retinal Ganglion Cell Damage Invest. Ophthalmol. Vis. Sci., October 1, 2007; 48(10): 4580 - 4589. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A M Lobascio, F G Klinger, M L Scaldaferri, D Farini, and M De Felici Analysis of programmed cell death in mouse fetal oocytes Reproduction, August 1, 2007; 134(2): 241 - 252. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. S. Supinski and L. A. Callahan Free radical-mediated skeletal muscle dysfunction in inflammatory conditions J Appl Physiol, May 1, 2007; 102(5): 2056 - 2063. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Neekhra, S. Luthra, M. Chwa, G. Seigel, A. L. Gramajo, B. D. Kuppermann, and M. C. Kenney Caspase-8, -12, and -3 Activation by 7-Ketocholesterol in Retinal Neurosensory Cells Invest. Ophthalmol. Vis. Sci., March 1, 2007; 48(3): 1362 - 1367. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Li, R. E. Anderson, H. Tomita, R. Adler, X. Liu, D. J. Zack, and R. V. S. Rajala Nonredundant Role of Akt2 for Neuroprotection of Rod Photoreceptor Cells from Light-Induced Cell Death J. Neurosci., January 3, 2007; 27(1): 203 - 211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kanan, G. Moiseyev, N. Agarwal, J.-X. Ma, and M. R. Al-Ubaidi Light Induces Programmed Cell Death by Activating Multiple Independent Proteases in a Cone Photoreceptor Cell Line Invest. Ophthalmol. Vis. Sci., January 1, 2007; 48(1): 40 - 51. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Tiwari, K. J. Messer, and P. R. Mayeux Inducible Nitric Oxide Synthase and Apoptosis in Murine Proximal Tubule Epithelial Cells Toxicol. Sci., June 1, 2006; 91(2): 493 - 500. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Zhou, R. Kohl, B. Herr, R. Frank, and B. Brune Calpain Mediates a von Hippel-Lindau Protein-independent Destruction of Hypoxia-inducible Factor-1{alpha} Mol. Biol. Cell, April 1, 2006; 17(4): 1549 - 1558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Doonan, M. Donovan, and T. G. Cotter Activation of Multiple Pathways during Photoreceptor Apoptosis in the rd Mouse Invest. Ophthalmol. Vis. Sci., October 1, 2005; 46(10): 3530 - 3538. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. E. Broker, F. A.E. Kruyt, and G. Giaccone Cell Death Independent of Caspases: A Review Clin. Cancer Res., May 1, 2005; 11(9): 3155 - 3162. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
