HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 38, 39628-39635, September 17, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/38/39628    most recent M405003200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hobson, R. P. Articles by Gow, N. A.R. Search for Related Content PubMed PubMed Citation Articles by Hobson, R. P. Articles by Gow, N. A.R. Social Bookmarking                      What's this?

Loss of Cell Wall Mannosylphosphate in Candida albicans Does Not Influence Macrophage Recognition^*

Richard P. Hobson, Carol A. Munro, Steven Bates, Donna M. MacCallum, Jim E. Cutler¶, Sigrid E. M. Heinsbroek||, Gordon D. Brown||, Frank C. Odds, and Neil A.R. Gow**

From the School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen, AB25 2ZD, United Kingdom, the ^¶Research Institute for Children, Children's Hospital, New Orleans, Louisiana 70118, and the ^||Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, United Kingdom

The outer layer of the cell wall of the human pathogenic fungus Candida albicans is enriched with heavily mannosylated glycoproteins that are the immediate point of contact between the fungus and cells of the host, including phagocytes. Previous work had identified components of the acid-labile fraction of N-linked mannan, comprising -1,2-linked mannose residues attached via a phosphodiester bond, as potential ligands for macrophage receptors and modulators of macrophage function. We therefore isolated and disrupted the CaMNN4 gene, which is required for mannosyl phosphate transfer and hence the attachment of -1,2 mannose oligosaccharides to the acid-labile N-mannan side chains. With the mannosylphosphate eliminated, the mnn4 null mutant was unable to bind the charged cationic dye Alcian Blue and was devoid of acid-labile -1,2-linked oligomannosaccharides. The mnn4 mutant was unaffected in cell growth and morphogenesis in vitro and in virulence in a murine model of systemic C. albicans infection. The null mutant was also not affected in its interaction with macrophages. Mannosylphosphate is therefore not required for macrophage interactions or for virulence of C. albicans.

Received for publication, May 5, 2004 , and in revised form, July 21, 2004.

^* This work was supported by a fellowship from the Royal College of Pathologists/Association of Clinical Pathologists (to R. P. H.) and Well-come Trust Grants 063204 and 72263 and by NIAID, National Institutes of Health Grant RO1 24912 (to J. E. C.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Present address: Mycology Reference Centre, Leeds General Infirmary, Leeds, LS1 3EX, UK.

^** To whom correspondence should be addressed. Tel.: 44-1224-555879; Fax: 44-1224-555844; E-mail n.gow{at}abdn.ac.uk.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Harris, H. M. Mora-Montes, N. A. R. Gow, and P. J. Coote Loss of mannosylphosphate from Candida albicans cell wall proteins results in enhanced resistance to the inhibitory effect of a cationic antimicrobial peptide via reduced peptide binding to the cell surface Microbiology, April 1, 2009; 155(4): 1058 - 1070. [Abstract] [Full Text] [PDF]

				D. M. MacCallum, L. Castillo, K. Nather, C. A. Munro, A. J. P. Brown, N. A. R. Gow, and F. C. Odds Property Differences among the Four Major Candida albicans Strain Clades Eukaryot. Cell, March 1, 2009; 8(3): 373 - 387. [Abstract] [Full Text] [PDF]

				A. Cambi, M. G. Netea, H. M. Mora-Montes, N. A. R. Gow, S. V. Hato, D. W. Lowman, B.-J. Kullberg, R. Torensma, D. L. Williams, and C. G. Figdor Dendritic Cell Interaction with Candida albicans Critically Depends on N-Linked Mannan J. Biol. Chem., July 18, 2008; 283(29): 20590 - 20599. [Abstract] [Full Text] [PDF]

				C. Mille, P. Bobrowicz, P.-A. Trinel, H. Li, E. Maes, Y. Guerardel, C. Fradin, M. Martinez-Esparza, R. C. Davidson, G. Janbon, et al. Identification of a New Family of Genes Involved in {beta}-1,2-Mannosylation of Glycans in Pichia pastoris and Candida albicans J. Biol. Chem., April 11, 2008; 283(15): 9724 - 9736. [Abstract] [Full Text] [PDF]

				H. M. Mora-Montes, S. Bates, M. G. Netea, D. F. Diaz-Jimenez, E. Lopez-Romero, S. Zinker, P. Ponce-Noyola, B. J. Kullberg, A. J. P. Brown, F. C. Odds, et al. Endoplasmic Reticulum {alpha}-Glycosidases of Candida albicans Are Required for N Glycosylation, Cell Wall Integrity, and Normal Host-Fungus Interaction Eukaryot. Cell, December 1, 2007; 6(12): 2184 - 2193. [Abstract] [Full Text] [PDF]

				K. C Hazen, D. R Singleton, and J. Masuoka Influence of outer region mannosylphosphorylation on N-glycan formation by Candida albicans: Normal acid-stable N-glycan formation requires acid-labile mannosylphosphate addition Glycobiology, October 1, 2007; 17(10): 1052 - 1060. [Abstract] [Full Text] [PDF]

				S. Bates, J. M. de la Rosa, D. M. MacCallum, A. J. P. Brown, N. A. R. Gow, and F. C. Odds Candida albicans Iff11, a Secreted Protein Required for Cell Wall Structure and Virulence Infect. Immun., June 1, 2007; 75(6): 2922 - 2928. [Abstract] [Full Text] [PDF]

				T. Jouault, M. El Abed-El Behi, M. Martinez-Esparza, L. Breuilh, P.-A. Trinel, M. Chamaillard, F. Trottein, and D. Poulain Specific Recognition of Candida albicans by Macrophages Requires Galectin-3 to Discriminate Saccharomyces cerevisiae and Needs Association with TLR2 for Signaling J. Immunol., October 1, 2006; 177(7): 4679 - 4687. [Abstract] [Full Text] [PDF]

				C. Bai, X.-L. Xu, F.-Y. Chan, R. T. H. Lee, and Y. Wang MNN5 Encodes an Iron-Regulated {alpha}-1,2-Mannosyltransferase Important for Protein Glycosylation, Cell Wall Integrity, Morphogenesis, and Virulence in Candida albicans Eukaryot. Cell, February 1, 2006; 5(2): 238 - 247. [Abstract] [Full Text] [PDF]

				S. Bates, H. B. Hughes, C. A. Munro, W. P. H. Thomas, D. M. MacCallum, G. Bertram, A. Atrih, M. A. J. Ferguson, A. J. P. Brown, F. C. Odds, et al. Outer Chain N-Glycans Are Required for Cell Wall Integrity and Virulence of Candida albicans J. Biol. Chem., January 6, 2006; 281(1): 90 - 98. [Abstract] [Full Text] [PDF]

				S. Bates, D. M. MacCallum, G. Bertram, C. A. Munro, H. B. Hughes, E. T. Buurman, A. J. P. Brown, F. C. Odds, and N. A. R. Gow Candida albicans Pmr1p, a Secretory Pathway P-type Ca2+/Mn2+-ATPase, Is Required for Glycosylation and Virulence J. Biol. Chem., June 17, 2005; 280(24): 23408 - 23415. [Abstract] [Full Text] [PDF]

				D. R. Singleton, J. Masuoka, and K. C. Hazen Surface Hydrophobicity Changes of Two Candida albicans Serotype B mnn4{Delta} Mutants Eukaryot. Cell, April 1, 2005; 4(4): 639 - 648. [Abstract] [Full Text] [PDF]

				C. A. Munro, S. Bates, E. T. Buurman, H. B. Hughes, D. M. MacCallum, G. Bertram, A. Atrih, M. A. J. Ferguson, J. M. Bain, A. Brand, et al. Mnt1p and Mnt2p of Candida albicans Are Partially Redundant {alpha}-1,2-Mannosyltransferases That Participate in O-Linked Mannosylation and Are Required for Adhesion and Virulence J. Biol. Chem., January 14, 2005; 280(2): 1051 - 1060. [Abstract] [Full Text] [PDF]