HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 38, 39677-39685, September 17, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/38/39677    most recent M403310200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Valadi, A. Articles by Adler, L. Search for Related Content PubMed PubMed Citation Articles by Valadi, A. Articles by Adler, L. Social Bookmarking                      What's this?

Distinct Intracellular Localization of Gpd1p and Gpd2p, the Two Yeast Isoforms of NAD⁺-dependent Glycerol-3-phosphate Dehydrogenase, Explains Their Different Contributions to Redox-driven Glycerol Production^*

Åsa Valadi, Katarina Granath, Lena Gustafsson, and Lennart Adler¶

From the Department of Cell and Molecular Biology/Microbiology, Box 462, SE-405 30 Göteborg, Sweden and the Department of Molecular Biotechnology, Chalmers University of Technology, Box 462, SE-405 30 Göteborg, Sweden

During anaerobiosis Saccharomyces cerevisiae strongly increases glycerol production to provide for non-respiratory oxidation of NADH to NAD⁺. We here report that respiratory-deficient cells become strictly dependent on the Gpd2p isoform of the NAD⁺-linked glycerol-3-phosphate dehydrogenase (Gpd). The growth inhibition of respiratory incompetent cox18 cells lacking GPD2 is reversed by the addition of acetoin, an alternative sink for NADH oxidation. Growth is also restored by addition of lysine or glutamic acid/glutamine, the synthesis of which involves production of mitochondrial NADH. Lysine produced a stronger growth stimulating effect than glutamic acid consistent with an upregulated expression of the IDP3 gene for peroxisomal synthesis of the glutamate precursor -ketoglutarate. Gpd2p is known to be a cytosolic protein but possesses a classical mitochondrial presequence, which we show is sufficient for mitochondrial targeting. A partial mitochondrial localization of Gpd2p will provide for establishment of intramitochondrial redox balance under non-respiratory conditions. Gpd1p, the other Gpd isoform, is partly cytosolic and partly peroxisomal and becomes more strictly peroxisomal in respiratory-deficient mutants. The different cellular distribution of Gpd1p and Gpd2p thus appears to be the main reason Gpd1p cannot substitute for Gpd2p in cox18gpd2 cells, despite similar kinetic characteristics of the two iso-enzymes.

Received for publication, March 24, 2004 , and in revised form, June 8, 2004.

^* This work was supported by grants from the Swedish Research Council. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence should be addressed. Tel.: 46-31-7732572; Fax: 46-31-7732599; E-mail: Lennart.Adler{at}gmm.gu.se.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Ehsani, M. R. Fernandez, J. A. Biosca, A. Julien, and S. Dequin Engineering of 2,3-Butanediol Dehydrogenase To Reduce Acetoin Formation by Glycerol-Overproducing, Low-Alcohol Saccharomyces cerevisiae Appl. Envir. Microbiol., May 15, 2009; 75(10): 3196 - 3205. [Abstract] [Full Text] [PDF]

				E. Nevoigt Progress in Metabolic Engineering of Saccharomyces cerevisiae Microbiol. Mol. Biol. Rev., September 1, 2008; 72(3): 379 - 412. [Abstract] [Full Text] [PDF]

				G. N. Vemuri, M. A. Eiteman, J. E. McEwen, L. Olsson, and J. Nielsen Increasing NADH oxidation reduces overflow metabolism in Saccharomyces cerevisiae PNAS, February 13, 2007; 104(7): 2402 - 2407. [Abstract] [Full Text] [PDF]

				I. Bourges, S. Horan, and B. Meunier Effect of Inhibition of the bc1 Complex on Gene Expression Profile in Yeast J. Biol. Chem., August 19, 2005; 280(33): 29743 - 29749. [Abstract] [Full Text] [PDF]

				C. Ferreira, F. van Voorst, A. Martins, L. Neves, R. Oliveira, M. C. Kielland-Brandt, C. Lucas, and A. Brandt A Member of the Sugar Transporter Family, Stl1p Is the Glycerol/H+ Symporter in Saccharomyces cerevisiae Mol. Biol. Cell, April 1, 2005; 16(4): 2068 - 2076. [Abstract] [Full Text] [PDF]

				T. A. Missall, J. M. Moran, J. A. Corbett, and J. K. Lodge Distinct Stress Responses of Two Functional Laccases in Cryptococcus neoformans Are Revealed in the Absence of the Thiol-Specific Antioxidant Tsa1 Eukaryot. Cell, January 1, 2005; 4(1): 202 - 208. [Abstract] [Full Text] [PDF]