| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 279, Issue 39, 40715-40722, September 24, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the
Departmento de Bioquímica, Facultad de Biología, Universidad Complutense, 28040 Madrid, Spain and the MEMPHYS-Center for Biomembrane Physics, the Department of Physics and the ¶Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense, Denmark
Pulmonary surfactant, the lipid-protein material that stabilizes the respiratory surface of the lungs, contains approximately equimolar amounts of saturated and unsaturated phospholipid species and significant proportions of cholesterol. Such lipid composition suggests that the membranes taking part in the surfactant structures could be organized heterogeneously in the form of inplane domains, originating from particular distributions of specific proteins and lipids. Here we report novel results concerning the lateral organization of bilayer membranes made of native pulmonary surfactant where the coexistence of two distinct micrometer sized fluid phases (fluid ordered and fluid disordered-like phases) is observed at physiological temperatures by using fluorescence microscopy and atomic force microscopy. Additional experiments using fluorescent-labeled proteins SP-B and SP-C show that at physiological temperatures these hydrophobic proteins are located exclusively in the fluid disordered-like phase. Most interestingly, the microscopic coexistence of fluid phases is maintained up to 37.5 °C, where most fluid ordered phases melt. This observation suggests that the particular composition of this material is naturally designed to be at the "edge" of a lateral structure transition under physiological conditions, likely providing particular structural and dynamic properties for its mechanical function. The observed lateral structure in native pulmonary surfactant membranes is dramatically affected by the extraction of cholesterol, an effect not observed upon extraction of the surfactant proteins. Furthermore, the spreading properties of the native surfactant material at the air-liquid interface were also greatly affected by cholesterol extraction, suggesting a connection between the observed lateral structure and a physiologically relevant function of the material. We suggest that the particular lipid composition of surfactant could be finely tuned to provide, under physiological conditions, a structural scaffold for surfactant proteins to act at appropriate local densities and lipid composition.
Received for publication, April 27, 2004 , and in revised form, June 23, 2004.
* This work was supported by Dirección General de Educación Superior e Investigaciones Científicas Grant BIO2003-09056 (to J. B. S. and J. P.-G.), Comunidad Autónoma de Madrid Grant 08.2/0054, and by Danish Natural Science Research Council Grant 21-03-0569 (to L. A. B.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
|| To whom correspondence should be addressed: MEMPHYS-Center for Biomembrane Physics, Dept. of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark. Tel.: 45-65-50-34-76; Fax: 45-66-15-87-60; E-mail: bagatolli{at}memphys.sdu.dk.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. C. Simonsen Activation of Phospholipase A2 by Ternary Model Membranes Biophys. J., May 15, 2008; 94(10): 3966 - 3975. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Y. Zuo, E. Keating, L. Zhao, S. M. Tadayyon, R. A. W. Veldhuizen, N. O. Petersen, and F. Possmayer Atomic Force Microscopy Studies of Functional and Dysfunctional Pulmonary Surfactant Films. I. Micro- and Nanostructures of Functional Pulmonary Surfactant Films and the Effect of SP-A Biophys. J., May 1, 2008; 94(9): 3549 - 3564. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Lhert, W. Yan, S. C. Biswas, and S. B. Hall Effects of Hydrophobic Surfactant Proteins on Collapse of Pulmonary Surfactant Monolayers Biophys. J., December 15, 2007; 93(12): 4237 - 4243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Rosetti and B. Maggio Protein-Induced Surface Structuring in Myelin Membrane Monolayers Biophys. J., December 15, 2007; 93(12): 4254 - 4267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-R. Montes, A. Alonso, F. M. Goni, and L. A. Bagatolli Giant Unilamellar Vesicles Electroformed from Native Membranes and Organic Lipid Mixtures under Physiological Conditions Biophys. J., November 15, 2007; 93(10): 3548 - 3554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Plasencia, L. Norlen, and L. A. Bagatolli Direct Visualization of Lipid Domains in Human Skin Stratum Corneum's Lipid Membranes: Effect of pH and Temperature Biophys. J., November 1, 2007; 93(9): 3142 - 3155. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Keating, L. Rahman, J. Francis, A. Petersen, F. Possmayer, R. Veldhuizen, and N. O. Petersen Effect of Cholesterol on the Biophysical and Physiological Properties of a Clinical Pulmonary Surfactant Biophys. J., August 15, 2007; 93(4): 1391 - 1401. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Sanchez, M. A. Tricerri, and E. Gratton Interaction of high density lipoprotein particles with membranes containing cholesterol J. Lipid Res., August 1, 2007; 48(8): 1689 - 1700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. F. M. de Almeida, J. Borst, A. Fedorov, M. Prieto, and A. J. W. G. Visser Complexity of Lipid Domains and Rafts in Giant Unilamellar Vesicles Revealed by Combining Imaging and Microscopic and Macroscopic Time-Resolved Fluorescence Biophys. J., July 15, 2007; 93(2): 539 - 553. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Markart, C. Ruppert, M. Wygrecka, T. Colaris, B. Dahal, D. Walmrath, H. Harbach, J. Wilhelm, W. Seeger, R. Schmidt, et al. Patients with ARDS show improvement but not normalisation of alveolar surface activity with surfactant treatment: putative role of neutral lipids Thorax, July 1, 2007; 62(7): 588 - 594. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Y. Zuo and F. Possmayer How does pulmonary surfactant reduce surface tension to very low values? J Appl Physiol, May 1, 2007; 102(5): 1733 - 1734. [Full Text] [PDF]
|
|
|
|
|
|
A. Saenz, O. Canadas, L. A. Bagatolli, F. Sanchez-Barbero, M. E. Johnson, and C. Casals Effect of Surfactant Protein A on the Physical Properties and Surface Activity of KL4-Surfactant Biophys. J., January 15, 2007; 92(2): 482 - 492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Slade, J. S. Schoeniger, D. Y. Sasaki, and C. M. Yip In Situ Scanning Probe Microscopy Studies of Tetanus Toxin-Membrane Interactions Biophys. J., December 15, 2006; 91(12): 4565 - 4574. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fidorra, L. Duelund, C. Leidy, A. C. Simonsen, and L.A. Bagatolli Absence of Fluid-Ordered/Fluid-Disordered Phase Coexistence in Ceramide/POPC Mixtures Containing Cholesterol Biophys. J., June 15, 2006; 90(12): 4437 - 4451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nag, K. M. W. Keough, and M. R. Morrow Probing Perturbation of Bovine Lung Surfactant Extracts by Albumin using DSC and 2H-NMR Biophys. J., May 15, 2006; 90(10): 3632 - 3642. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Lang, A. D. Postle, S. Orgeig, F. Possmayer, W. Bernhard, A. K. Panda, K. D. Jurgens, W. K. Milsom, K. Nag, and C. B. Daniels Dipalmitoylphosphatidylcholine is not the major surfactant phospholipid species in all mammals Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2005; 289(5): R1426 - R1439. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Oradd, P. W. Westerman, and G. Lindblom Lateral Diffusion Coefficients of Separate Lipid Species in a Ternary Raft-Forming Bilayer: A Pfg-NMR Multinuclear Study Biophys. J., July 1, 2005; 89(1): 315 - 320. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
