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J. Biol. Chem., Vol. 279, Issue 39, 40852-40860, September 24, 2004

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The ATC1 Gene Encodes a Cell Wall-linked Acid Trehalase Required for Growth on Trehalose in Candida albicans^*

Yolanda Pedreño¶, Sergi Maicas||, Juan-Carlos Argüelles, Rafael Sentandreu, and Eulogio Valentin**

From the Area de Microbiología, Facultad de Biología, Universidad de Murcia, 30071 Murcia, Spain, ^||Facultad de Ciencias Experimentales y de la Salud, Universidad Cardenal Herrera-CEU, 46113 Moncada, Valencia, Spain, and Departamento de Microbiología y Ecología, Universidad de Valencia, 46100 Burjassot, Valencia, Spain

After screening a Candida albicans genome data base, the product of an open reading frame (IPF 19760/CA2574) with 41% identity to Saccharomyces cerevisiae vacuolar acid trehalase (Ath1p) was identified and named Atc1p. The deduced amino acid sequence shows that Atc1p contains an N-terminal hydrophobic signal peptide and 20 potential sites for N-glycosylation. C. albicans homozygous mutants that lack acid trehalase activity were constructed by gene disruption at the two ATC chromosomal alleles. Analysis of these null mutants shows that Atc1p is localized in the cell wall and is required for growth on trehalose as a carbon source. An Atc1p endowed with acid trehalase activity was obtained by an in vtro transcription-translation coupled system. These results strongly suggest that ATC1 is the structural gene encoding cell wall acid trehalase in C. albicans. Determinations of ATC1 mRNA expression as well as acid trehalase activity in the presence and absence of glucose point out that ATC1 gene is regulated by glucose repression.

Received for publication, January 9, 2004 , and in revised form, July 7, 2004.

^* This work was supported in part by Ministerio de Ciencia y Tecnologia (Spain) Grant BMC2003-01023, the European Union Grant MRTN-CT-2003-504148, Fundación Séneca (Comunidad de Murcia, Spain) Grant PB/07/FS/02, and the Agéncia Valenciana de Ciéncia i Tecnologia, Generalitat Valenciana (Comunidad Valenciana, Spain) Grant Grupos 03/187. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ Supported by CESPA-Ingeniería Urbana (Spain).

^** To whom correspondence should be addressed: Departamento de Microbiología y Ecología, Facultad de Farmacia, Universidad de Valencia, Avda. Vicente Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain. Tel.: 34-963543614; Fax: 34-963544299; E-mail: Eulogio.Valentin{at}uv.es.

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