HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 39, 40882-40889, September 24, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/39/40882    most recent M400466200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Triantafilou, M. Articles by Triantafilou, K. Search for Related Content PubMed PubMed Citation Articles by Triantafilou, M. Articles by Triantafilou, K. Social Bookmarking                      What's this?

Lipoteichoic Acid and Toll-like Receptor 2 Internalization and Targeting to the Golgi Are Lipid Raft-dependent^*

Martha Triantafilou, Maria Manukyan, Alan Mackie¶, Siegfried Morath||, Thomas Hartung||, Holger Heine, and Kathy Triantafilou**

From the Infection and Immunity Group, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG, United Kingdom, the Department of Immunology and Cell Biology, Research Center Borstel, Parkallee 22, Borstel D-23845, Germany, the ^¶Department of Food Material Science, Institute of Food Research, Norwich Research Park, Norwich NR4 7UA, United Kingdom, and the ^||Department of Biochemical Pharmacology, University of Konstanz, Konstanz D-78457, Germany

Lipoteichoic acid (LTA), a key cell wall component of Gram-positive bacteria, seems to function as an immune activator with characteristics very similar to lipopolysaccharide from Gram-negative bacteria. It has been shown that LTA binds CD14 and triggers activation via Toll-like receptor 2, but whether the activation occurs at the cell surface or internalization is required to trigger signaling has yet to be demonstrated. In this work we have investigated LTA binding and internalization and found that LTA and its receptor molecules accumulate in lipid rafts and are subsequently targeted rapidly to the Golgi apparatus. This internalization seems to be lipid raft-dependent because raft-disrupting drugs inhibited LTA/Toll-like receptor 2 colocalization in the Golgi. Similarly to lipopolysaccharide, LTA activation occurs at the cell surface, and the observed trafficking is independent of signaling.

Received for publication, January 15, 2004 , and in revised form, July 6, 2004.

^* This work was supported by the Wellcome Trust (to K. T.) and German Research Foundation Grant He 2758/3-1 (to H. H.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^** To whom correspondence should be addressed: School of Life Sciences, University of Sussex, JMS Bldg., Falmer, Brighton BN1 9QG, United Kingdom. Tel.: 44-1273-678-362 or 923; Fax: 44-1273-678-362; E-mail: K.Triantafilou{at}sussex.ac.uk.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A.-C. Raby, E. Le Bouder, C. Colmont, J. Davies, P. Richards, B. Coles, C. H. George, S. A. Jones, P. Brennan, N. Topley, et al. Soluble TLR2 Reduces Inflammation without Compromising Bacterial Clearance by Disrupting TLR2 Triggering J. Immunol., July 1, 2009; 183(1): 506 - 517. [Abstract] [Full Text] [PDF]

				L. C. Parker, E. C. Prestwich, J. R. Ward, E. Smythe, A. Berry, M. Triantafilou, K. Triantafilou, and I. Sabroe A Phosphatidylserine Species Inhibits a Range of TLR- but Not IL-1{beta}-Induced Inflammatory Responses by Disruption of Membrane Microdomains J. Immunol., October 15, 2008; 181(8): 5606 - 5617. [Abstract] [Full Text] [PDF]

				S. Meron-Sudai, A. Matityahou, Y. Keisari, K. H. Cox, D. L. Hasty, and I. Ofek Lipoteichoic Acid Synergizes with Glycosphingolipids To Potently Stimulate Secretion of Interleukin-6 from Human Blood Cells Clin. Vaccine Immunol., September 1, 2008; 15(9): 1309 - 1315. [Abstract] [Full Text] [PDF]

				N. J. Nilsen, S. Deininger, U. Nonstad, F. Skjeldal, H. Husebye, D. Rodionov, S. von Aulock, T. Hartung, E. Lien, O. Bakke, et al. Cellular trafficking of lipoteichoic acid and Toll-like receptor 2 in relation to signaling; role of CD14 and CD36 J. Leukoc. Biol., July 1, 2008; 84(1): 280 - 291. [Abstract] [Full Text] [PDF]

				L. K. Juckem, K. W. Boehme, A. L. Feire, and T. Compton Differential Initiation of Innate Immune Responses Induced by Human Cytomegalovirus Entry into Fibroblast Cells J. Immunol., April 1, 2008; 180(7): 4965 - 4977. [Abstract] [Full Text] [PDF]

				S. Knapp, S. von Aulock, M. Leendertse, I. Haslinger, C. Draing, D. T. Golenbock, and T. van der Poll Lipoteichoic Acid-Induced Lung Inflammation Depends on TLR2 and the Concerted Action of TLR4 and the Platelet-Activating Factor Receptor J. Immunol., March 1, 2008; 180(5): 3478 - 3484. [Abstract] [Full Text] [PDF]

				H. S. Seo, S. M. Michalek, and M. H. Nahm Lipoteichoic Acid Is Important in Innate Immune Responses to Gram-Positive Bacteria Infect. Immun., January 1, 2008; 76(1): 206 - 213. [Abstract] [Full Text] [PDF]

				G. S. Ali, K. V. S. K. Prasad, I. Day, and A. S. N. Reddy Ligand-Dependent Reduction in the Membrane Mobility of FLAGELLIN SENSITIVE2, an Arabidopsis Receptor-Like Kinase Plant Cell Physiol., November 1, 2007; 48(11): 1601 - 1611. [Abstract] [Full Text] [PDF]

				Shuang Chen, M. H. Wong, D. J. Schulte, M. Arditi, and K. S. Michelsen Differential expression of Toll-like receptor 2 (TLR2) and responses to TLR2 ligands between human and murine vascular endothelial cells Innate Immunity, October 1, 2007; 13(5): 281 - 296. [Abstract] [PDF]

				S. Khan, M. S. Bijker, J. J. Weterings, H. J. Tanke, G. J. Adema, T. van Hall, J. W. Drijfhout, C. J. M. Melief, H. S. Overkleeft, G. A. van der Marel, et al. Distinct Uptake Mechanisms but Similar Intracellular Processing of Two Different Toll-like Receptor Ligand-Peptide Conjugates in Dendritic Cells J. Biol. Chem., July 20, 2007; 282(29): 21145 - 21159. [Abstract] [Full Text] [PDF]

				J. H. Chang, T. Hampartzoumian, B. Everett, A. Lloyd, P. J. McCluskey, and D. Wakefield Changes in Toll-like Receptor (TLR)-2 and TLR4 Expression and Function but Not Polymorphisms Are Associated with Acute Anterior Uveitis Invest. Ophthalmol. Vis. Sci., April 1, 2007; 48(4): 1711 - 1717. [Abstract] [Full Text] [PDF]

				G. Szabo, A. Dolganiuc, Q. Dai, and S. B. Pruett TLR4, Ethanol, and Lipid Rafts: A New Mechanism of Ethanol Action with Implications for other Receptor-Mediated Effects J. Immunol., February 1, 2007; 178(3): 1243 - 1249. [Abstract] [Full Text] [PDF]

				J. J. Lasarte, N. Casares, M. Gorraiz, S. Hervas-Stubbs, L. Arribillaga, C. Mansilla, M. Durantez, D. Llopiz, P. Sarobe, F. Borras-Cuesta, et al. The Extra Domain A from Fibronectin Targets Antigens to TLR4-Expressing Cells and Induces Cytotoxic T Cell Responses In Vivo J. Immunol., January 15, 2007; 178(2): 748 - 756. [Abstract] [Full Text] [PDF]

				Y. Sakata, J.-W. Dong, J. G. Vallejo, C.-H. Huang, J. S. Baker, K. J. Tracey, O. Tacheuchi, S. Akira, and D. L. Mann Toll-like receptor 2 modulates left ventricular function following ischemia-reperfusion injury Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H503 - H509. [Abstract] [Full Text] [PDF]

				C. Draing, M. Pfitzenmaier, S. Zummo, G. Mancuso, A. Geyer, T. Hartung, and S. von Aulock Comparison of Lipoteichoic Acid from Different Serotypes of Streptococcus pneumoniae J. Biol. Chem., November 10, 2006; 281(45): 33849 - 33859. [Abstract] [Full Text] [PDF]

				M. Triantafilou, F. G. J. Gamper, R. M. Haston, M. A. Mouratis, S. Morath, T. Hartung, and K. Triantafilou Membrane Sorting of Toll-like Receptor (TLR)-2/6 and TLR2/1 Heterodimers at the Cell Surface Determines Heterotypic Associations with CD36 and Intracellular Targeting J. Biol. Chem., October 13, 2006; 281(41): 31002 - 31011. [Abstract] [Full Text] [PDF]

				F.-S. X. Yu and L. D. Hazlett Toll-like Receptors and the Eye. Invest. Ophthalmol. Vis. Sci., April 1, 2006; 47(4): 1255 - 1263. [Full Text] [PDF]

				S. Chabot, J. S. Wagner, S. Farrant, and M. R. Neutra TLRs Regulate the Gatekeeping Functions of the Intestinal Follicle-Associated Epithelium J. Immunol., April 1, 2006; 176(7): 4275 - 4283. [Abstract] [Full Text] [PDF]

				M. Triantafilou and K. Triantafilou Invited review: The dynamics of LPS recognition: complex orchestration of multiple receptors Innate Immunity, February 1, 2005; 11(1): 5 - 11. [Abstract] [PDF]