|
Originally published In Press as doi:10.1074/jbc.M400466200 on July 9, 2004
J. Biol. Chem., Vol. 279, Issue 39, 40882-40889, September 24, 2004
Lipoteichoic Acid and Toll-like Receptor 2 Internalization and Targeting to the Golgi Are Lipid Raft-dependent*
Martha Triantafilou ,
Maria Manukyan ,
Alan Mackie¶,
Siegfried Morath||,
Thomas Hartung||,
Holger Heine , and
Kathy Triantafilou **
From the
Infection and Immunity Group, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG, United Kingdom, the Department of Immunology and Cell Biology, Research Center Borstel, Parkallee 22, Borstel D-23845, Germany, the ¶Department of Food Material Science, Institute of Food Research, Norwich Research Park, Norwich NR4 7UA, United Kingdom, and the ||Department of Biochemical Pharmacology, University of Konstanz, Konstanz D-78457, Germany
Lipoteichoic acid (LTA), a key cell wall component of Gram-positive bacteria, seems to function as an immune activator with characteristics very similar to lipopolysaccharide from Gram-negative bacteria. It has been shown that LTA binds CD14 and triggers activation via Toll-like receptor 2, but whether the activation occurs at the cell surface or internalization is required to trigger signaling has yet to be demonstrated. In this work we have investigated LTA binding and internalization and found that LTA and its receptor molecules accumulate in lipid rafts and are subsequently targeted rapidly to the Golgi apparatus. This internalization seems to be lipid raft-dependent because raft-disrupting drugs inhibited LTA/Toll-like receptor 2 colocalization in the Golgi. Similarly to lipopolysaccharide, LTA activation occurs at the cell surface, and the observed trafficking is independent of signaling.
Received for publication, January 15, 2004
, and in revised form, July 6, 2004.
* This work was supported by the Wellcome Trust (to K. T.) and German Research Foundation Grant He 2758/3-1 (to H. H.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
** To whom correspondence should be addressed: School of Life Sciences, University of Sussex, JMS Bldg., Falmer, Brighton BN1 9QG, United Kingdom. Tel.: 44-1273-678-362 or 923; Fax: 44-1273-678-362; E-mail: K.Triantafilou{at}sussex.ac.uk.

CiteULike Complore Connotea Del.icio.us Digg Reddit Technorati What's this?
This article has been cited by other articles:

|
 |

|
 |
 
A.-C. Raby, E. Le Bouder, C. Colmont, J. Davies, P. Richards, B. Coles, C. H. George, S. A. Jones, P. Brennan, N. Topley, et al.
Soluble TLR2 Reduces Inflammation without Compromising Bacterial Clearance by Disrupting TLR2 Triggering
J. Immunol.,
July 1, 2009;
183(1):
506 - 517.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
L. C. Parker, E. C. Prestwich, J. R. Ward, E. Smythe, A. Berry, M. Triantafilou, K. Triantafilou, and I. Sabroe
A Phosphatidylserine Species Inhibits a Range of TLR- but Not IL-1{beta}-Induced Inflammatory Responses by Disruption of Membrane Microdomains
J. Immunol.,
October 15, 2008;
181(8):
5606 - 5617.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Meron-Sudai, A. Matityahou, Y. Keisari, K. H. Cox, D. L. Hasty, and I. Ofek
Lipoteichoic Acid Synergizes with Glycosphingolipids To Potently Stimulate Secretion of Interleukin-6 from Human Blood Cells
Clin. Vaccine Immunol.,
September 1, 2008;
15(9):
1309 - 1315.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
N. J. Nilsen, S. Deininger, U. Nonstad, F. Skjeldal, H. Husebye, D. Rodionov, S. von Aulock, T. Hartung, E. Lien, O. Bakke, et al.
Cellular trafficking of lipoteichoic acid and Toll-like receptor 2 in relation to signaling; role of CD14 and CD36
J. Leukoc. Biol.,
July 1, 2008;
84(1):
280 - 291.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
L. K. Juckem, K. W. Boehme, A. L. Feire, and T. Compton
Differential Initiation of Innate Immune Responses Induced by Human Cytomegalovirus Entry into Fibroblast Cells
J. Immunol.,
April 1, 2008;
180(7):
4965 - 4977.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Knapp, S. von Aulock, M. Leendertse, I. Haslinger, C. Draing, D. T. Golenbock, and T. van der Poll
Lipoteichoic Acid-Induced Lung Inflammation Depends on TLR2 and the Concerted Action of TLR4 and the Platelet-Activating Factor Receptor
J. Immunol.,
March 1, 2008;
180(5):
3478 - 3484.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
H. S. Seo, S. M. Michalek, and M. H. Nahm
Lipoteichoic Acid Is Important in Innate Immune Responses to Gram-Positive Bacteria
Infect. Immun.,
January 1, 2008;
76(1):
206 - 213.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
G. S. Ali, K. V. S. K. Prasad, I. Day, and A. S. N. Reddy
Ligand-Dependent Reduction in the Membrane Mobility of FLAGELLIN SENSITIVE2, an Arabidopsis Receptor-Like Kinase
Plant Cell Physiol.,
November 1, 2007;
48(11):
1601 - 1611.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Shuang Chen, M. H. Wong, D. J. Schulte, M. Arditi, and K. S. Michelsen
Differential expression of Toll-like receptor 2 (TLR2) and responses to TLR2 ligands between human and murine vascular endothelial cells
Innate Immunity,
October 1, 2007;
13(5):
281 - 296.
[Abstract]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Khan, M. S. Bijker, J. J. Weterings, H. J. Tanke, G. J. Adema, T. van Hall, J. W. Drijfhout, C. J. M. Melief, H. S. Overkleeft, G. A. van der Marel, et al.
Distinct Uptake Mechanisms but Similar Intracellular Processing of Two Different Toll-like Receptor Ligand-Peptide Conjugates in Dendritic Cells
J. Biol. Chem.,
July 20, 2007;
282(29):
21145 - 21159.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. H. Chang, T. Hampartzoumian, B. Everett, A. Lloyd, P. J. McCluskey, and D. Wakefield
Changes in Toll-like Receptor (TLR)-2 and TLR4 Expression and Function but Not Polymorphisms Are Associated with Acute Anterior Uveitis
Invest. Ophthalmol. Vis. Sci.,
April 1, 2007;
48(4):
1711 - 1717.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
G. Szabo, A. Dolganiuc, Q. Dai, and S. B. Pruett
TLR4, Ethanol, and Lipid Rafts: A New Mechanism of Ethanol Action with Implications for other Receptor-Mediated Effects
J. Immunol.,
February 1, 2007;
178(3):
1243 - 1249.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. J. Lasarte, N. Casares, M. Gorraiz, S. Hervas-Stubbs, L. Arribillaga, C. Mansilla, M. Durantez, D. Llopiz, P. Sarobe, F. Borras-Cuesta, et al.
The Extra Domain A from Fibronectin Targets Antigens to TLR4-Expressing Cells and Induces Cytotoxic T Cell Responses In Vivo
J. Immunol.,
January 15, 2007;
178(2):
748 - 756.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y. Sakata, J.-W. Dong, J. G. Vallejo, C.-H. Huang, J. S. Baker, K. J. Tracey, O. Tacheuchi, S. Akira, and D. L. Mann
Toll-like receptor 2 modulates left ventricular function following ischemia-reperfusion injury
Am J Physiol Heart Circ Physiol,
January 1, 2007;
292(1):
H503 - H509.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
C. Draing, M. Pfitzenmaier, S. Zummo, G. Mancuso, A. Geyer, T. Hartung, and S. von Aulock
Comparison of Lipoteichoic Acid from Different Serotypes of Streptococcus pneumoniae
J. Biol. Chem.,
November 10, 2006;
281(45):
33849 - 33859.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Triantafilou, F. G. J. Gamper, R. M. Haston, M. A. Mouratis, S. Morath, T. Hartung, and K. Triantafilou
Membrane Sorting of Toll-like Receptor (TLR)-2/6 and TLR2/1 Heterodimers at the Cell Surface Determines Heterotypic Associations with CD36 and Intracellular Targeting
J. Biol. Chem.,
October 13, 2006;
281(41):
31002 - 31011.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
F.-S. X. Yu and L. D. Hazlett
Toll-like Receptors and the Eye.
Invest. Ophthalmol. Vis. Sci.,
April 1, 2006;
47(4):
1255 - 1263.
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Chabot, J. S. Wagner, S. Farrant, and M. R. Neutra
TLRs Regulate the Gatekeeping Functions of the Intestinal Follicle-Associated Epithelium
J. Immunol.,
April 1, 2006;
176(7):
4275 - 4283.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Triantafilou and K. Triantafilou
Invited review: The dynamics of LPS recognition: complex orchestration of multiple receptors
Innate Immunity,
February 1, 2005;
11(1):
5 - 11.
[Abstract]
[PDF]
|
 |
|
Copyright © 2004 by the American Society for Biochemistry and Molecular Biology.
|
Advertisement
Advertisement
|