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J. Biol. Chem., Vol. 279, Issue 40, 41310-41318, October 1, 2004

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Changes in Plasma Membrane Properties and Phosphatidylcholine Subspecies of Insect Sf9 Cells Due to Expression of Scavenger Receptor Class B, Type I, and CD36^*

Saj Parathath||, Margery A. Connelly, Robert A. Rieger, Seth M. Klein, Nada A. Abumrad, Margarita de la Llera-Moya¶, Charles R. Iden, George H. Rothblat¶, and David L. Williams

From the Departments of Pharmacological Sciences and Physiology and Biophysics, University Medical Center, State University of New York, Stony Brook, New York 11794-8651 and ^¶Division of Gastroenterology and Nutrition, the Department of Pediatrics, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19104

In mammalian cells scavenger receptor class B, type I (SR-BI), mediates the selective uptake of high density lipoprotein (HDL) cholesteryl ester into hepatic and steroidogenic cells. In addition, SR-BI has a variety of effects on plasma membrane properties including stimulation of the bidirectional flux of free cholesterol (FC) between cells and HDL and changes in the organization of plasma membrane FC as indicated by increased susceptibility to exogenous cholesterol oxidase. Recent studies in SR-BI-deficient mice and in SR-BI-expressing Sf9 insect cells showed that SR-BI has significant effects on plasma membrane ultrastructure. The present study was designed to test the range of SR-BI effects in Sf9 insect cells that typically have very low cholesterol content and a different phospholipid profile compared with mammalian cells. The results showed that, as in mammalian cells, SR-BI expression increased HDL cholesteryl ester selective uptake, cellular cholesterol mass, FC efflux to HDL, and the sensitivity of membrane FC to cholesterol oxidase. These activities were diminished or absent upon expression of the related scavenger receptor CD36. Thus, SR-BI has fundamental effects on cholesterol flux and membrane properties that occur in cells of evolutionarily divergent origins. Profiling of phospholipid species by electrospray ionization mass spectrometry showed that scavenger receptor expression led to the accumulation of phosphatidylcholine species with longer mono- or polyunsaturated acyl chains. These changes would be expected to decrease phosphatidylcholine/cholesterol interactions and thereby enhance cholesterol desorption from the membrane. Scavenger receptor-mediated changes in membrane phosphatidylcholine may contribute to the increased flux of cholesterol and other lipids elicited by these receptors.

Received for publication, May 4, 2004 , and in revised form, July 6, 2004.

^* This work was supported by National Institutes of Health Grants HL63768, HL58012, and HL22633 and an Atorvastain research award (to M. A. C.) sponsored by Pfizer, Inc. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

We are deeply saddened by the sudden loss of our dear friend and colleague, Dr. David L. Williams, who passed away on July 16, 2004. Dave was a devoted scientist, teacher, and friend and will not be forgotten. His passing is a tremendous loss to all of us, and we will miss him dearly.

^|| To whom correspondence should be addressed: Dept. of Pharmacological Sciences, University Medical Center, State University of New York, Stony Brook, NY 11794-8651. Tel.: 631-444-9685; Fax: 631-444-3011; E-mail: parathath{at}pharm.sunysb.edu.

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