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J. Biol. Chem., Vol. 279, Issue 42, 43735-43743, October 15, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/42/43735    most recent M407544200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cao, Y. Articles by Knöchel, W. Search for Related Content PubMed PubMed Citation Articles by Cao, Y. Articles by Knöchel, W. Social Bookmarking                      What's this?

The POU Factor Oct-25 Regulates the Xvent-2B Gene and Counteracts Terminal Differentiation in Xenopus Embryos^*

Ying Cao, Sigrun Knöchel, Cornelia Donow, Josef Miethe, Eckhard Kaufmann, and Walter Knöchel

From the Abteilung Biochemie, Universität Ulm, Albert-Einstein-Allee 11, D-89081 Ulm, Germany

The Xvent-2B promoter is regulated by a BMP-2/4-induced transcription complex comprising Smad signal transducers and specific transcription factors. Using a yeast one-hybrid screen we have found that Oct-25, a Xenopus POU domain protein related to mammalian Oct-3/4, binds as an additional factor to the Xvent-2B promoter. This interaction was further confirmed by both in vitro and in vivo analyses. The Oct-25 gene is mainly transcribed during blastula and gastrula stages in the newly forming ectodermal and mesodermal germ layers. Luciferase reporter gene assay demonstrated that Oct-25 stimulates transcription of the Xvent-2B gene. This stimulation depends on the Oct-25 binding site and the bone morphogenetic protein-responsive element. Furthermore, Oct-25 interacts in vitro with components of the Xvent-2B transcription complex, like Smad1/4 and Xvent-2. Overexpression of Oct-25 results in anterior/posterior truncations and lack of differentiation for neuroectoderm- and mesoderm-derived tissues including blood cells. This effect is consistent with an evolutionarily conserved role of class V POU factors in the maintenance of an undifferentiated cell state. In Xenopus, the molecular mechanism underlying this process might be coupled to the expression of Xvent proteins.

Received for publication, July 7, 2004

^* This work was supported by Grant SFB 497/A 1 from the Deutsche Forschungsgemeinschaft and by the Fonds der Chemischen Industrie. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank^TM/EBI Data Bank with accession number(s) AJ699165.

To whom correspondence should be addressed. Tel.: 49-731-5023280; Fax: 49-731-5023277; E-mail: walter.knoechel{at}medizin.uniulm.de.

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