Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


Originally published In Press as doi:10.1074/jbc.M401646200 on July 22, 2004

J. Biol. Chem., Vol. 279, Issue 42, 44154-44165, October 15, 2004
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
279/42/44154    most recent
M401646200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Heilman-Miller, S. L.
Right arrow Articles by Levin, J. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Heilman-Miller, S. L.
Right arrow Articles by Levin, J. G.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Alteration of Nucleic Acid Structure and Stability Modulates the Efficiency of Minus-Strand Transfer Mediated by the HIV-1 Nucleocapsid Protein*

Susan L. Heilman-Miller, Tiyun Wu, and Judith G. Levin{ddagger}

From the Laboratory of Molecular Genetics, NICHD, National Institutes of Health, Bethesda, Maryland 20892-2780

During human immunodeficiency virus type 1 minus-strand transfer, the nucleocapsid protein (NC) facilitates annealing of the complementary repeat regions at the 3'-ends of acceptor RNA and minus-strand strong-stop DNA ((-) SSDNA). In addition, NC destabilizes the highly structured complementary trans-activation response element (TAR) stem-loop (TAR DNA) at the 3'-end of (-) SSDNA and inhibits TAR-induced self-priming, a dead-end reaction that competes with minus-strand transfer. To investigate the relationship between nucleic acid secondary structure and NC function, a series of truncated (-) SSDNA and acceptor RNA constructs were used to assay minus-strand transfer and self-priming in vitro. The results were correlated with extensive enzymatic probing and mFold analysis. As the length of (-) SSDNA was decreased, self-priming increased and was highest when the DNA contained little more than TAR DNA, even if NC and acceptor were both present; in contrast, truncations within TAR DNA led to a striking reduction or elimination of self-priming. However, destabilization of TAR DNA was not sufficient for successful strand transfer: the stability of acceptor RNA was also crucial, and little or no strand transfer occurred if the RNA was highly stable. Significantly, NC may not be required for in vitro strand transfer if (-) SSDNA and acceptor RNA are small, relatively unstructured molecules with low thermodynamic stabilities. Collectively, these findings demonstrate that for efficient NC-mediated minus-strand transfer, a delicate thermodynamic balance between the RNA and DNA reactants must be maintained.


Received for publication, February 13, 2004 , and in revised form, July 21, 2004.

* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

{ddagger} To whom correspondence should be addressed: Lab. of Molecular Genetics, NICHD, NIH, Bldg. 6B, Rm. 216, Bethesda, MD 20892-2780. Tel.: 301-496-1970; Fax: 301-496-0243; E-mail: levinju{at}mail.nih.gov.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
Nucleic Acids ResHome page
K. Post, B. Kankia, S. Gopalakrishnan, V. Yang, E. Cramer, P. Saladores, R. J. Gorelick, J. Guo, K. Musier-Forsyth, and J. G. Levin
Fidelity of plus-strand priming requires the nucleic acid chaperone activity of HIV-1 nucleocapsid protein
Nucleic Acids Res., April 1, 2009; 37(6): 1755 - 1766.
[Abstract] [Full Text] [PDF]


Home page
J. Virol.Home page
K. M. Stewart-Maynard, M. Cruceanu, F. Wang, M.-N. Vo, R. J. Gorelick, M. C. Williams, I. Rouzina, and K. Musier-Forsyth
Retroviral Nucleocapsid Proteins Display Nonequivalent Levels of Nucleic Acid Chaperone Activity
J. Virol., October 15, 2008; 82(20): 10129 - 10142.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
M. Song, V. P. Basu, M. N. Hanson, B. P. Roques, and R. A. Bambara
Proximity and Branch Migration Mechanisms in HIV-1 Minus Strand Strong Stop DNA Transfer
J. Biol. Chem., February 8, 2008; 283(6): 3141 - 3150.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
Y. Iwatani, D. S.B. Chan, F. Wang, K. S. Maynard, W. Sugiura, A. M. Gronenborn, I. Rouzina, M. C. Williams, K. Musier-Forsyth, and J. G. Levin
Deaminase-independent inhibition of HIV-1 reverse transcription by APOBEC3G
Nucleic Acids Res., December 18, 2007; 35(21): 7096 - 7108.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
T. Wu, S. L. Heilman-Miller, and J. G. Levin
Effects of nucleic acid local structure and magnesium ions on minus-strand transfer mediated by the nucleic acid chaperone activity of HIV-1 nucleocapsid protein
Nucleic Acids Res., June 9, 2007; 35(12): 3974 - 3987.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
L. Gao, M. Balakrishnan, B. P. Roques, and R. A. Bambara
Insights into the Multiple Roles of Pausing in HIV-1 Reverse Transcriptase-promoted Strand Transfers
J. Biol. Chem., March 2, 2007; 282(9): 6222 - 6231.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
M. Song, M. Balakrishnan, Y. Chen, B. P. Roques, and R. A. Bambara
Stimulation of HIV-1 Minus Strand Strong Stop DNA Transfer by Genomic Sequences 3' of the Primer Binding Site
J. Biol. Chem., August 25, 2006; 281(34): 24227 - 24235.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
R. Galetto, V. Giacomoni, M. Veron, and M. Negroni
Dissection of a Circumscribed Recombination Hot Spot in HIV-1 after a Single Infectious Cycle
J. Biol. Chem., February 3, 2006; 281(5): 2711 - 2720.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
M. Cruceanu, M. A. Urbaneja, C. V. Hixson, D. G. Johnson, S. A. Datta, M. J. Fivash, A. G. Stephen, R. J. Fisher, R. J. Gorelick, J. R. Casas-Finet, et al.
Nucleic acid binding and chaperone properties of HIV-1 Gag and nucleocapsid proteins
Nucleic Acids Res., January 30, 2006; 34(2): 593 - 605.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2004 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement