| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 279, Issue 45, 47066-47075, November 5, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California 94720
We have used rat cDNA microarrays to identify adipocyte-specific genes that could play an important role in adipocyte differentiation or function. Here, we report the cloning and identification of a 2.0-kb mRNA coding for a putative protein that we have designated as desnutrin. The novel gene is expressed predominantly in adipose tissue, and its expression is induced early during 3T3-L1 adipocyte differentiation. Desnutrin mRNA levels were regulated by the nutritional status of animals, being transiently induced during fasting. In vitro desnutrin gene expression was up-regulated by dexamethasone in a dose-dependent manner but not by cAMP, suggesting that glucocorticoids could mediate the increase in desnutrin mRNA levels observed during fasting. Desnutrin mRNA codes for a 486-amino acid putative protein containing a patatin-like domain, characteristic of many plant acyl hydrolases belonging to the patatin family. Confocal microscopy of enhanced green fluorescent protein-tagged desnutrin protein-transfected cells showed that the fusion protein localized in the cytoplasm. Moreover, cells overexpressing desnutrin by transfection showed an increase in triglyceride hydrolysis. Interestingly, we also found that the desnutrin gene expression level was lower in ob/ob and db/db obese mouse models. Overall, our data suggest that the newly identified desnutrin gene codes for an adipocyte protein that may function as a lipase and play a role in the adaptive response to a low energy state, such as fasting, by providing fatty acids to other tissues for oxidation. In addition, decreased expression of desnutrin in obesity models suggests its possible contribution to the pathophysiology of obesity.
Received for publication, April 7, 2004 , and in revised form, August 12, 2004.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AY731699
* This work was supported by National Institutes of Health Grants DK050828 and DK068439 (to H. S. S.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.: 510-642-3978; Fax: 510-642-0535; E-mail: hsul{at}nature.berkeley.edu.
This article has been cited by other articles:
|
|
 |
|
  B. N. Reid, G. P. Ables, O. A. Otlivanchik, G. Schoiswohl, R. Zechner, W. S. Blaner, I. J. Goldberg, R. F. Schwabe, S. C. Chua Jr., and L.-S. Huang Hepatic Overexpression of Hormone-sensitive Lipase and Adipose Triglyceride Lipase Promotes Fatty Acid Oxidation, Stimulates Direct Release of Free Fatty Acids, and Ameliorates Steatosis J. Biol. Chem., May 9, 2008; 283(19): 13087 - 13099. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. P Macfarlane, S. Forbes, and B. R Walker Glucocorticoids and fatty acid metabolism in humans: fuelling fat redistribution in the metabolic syndrome J. Endocrinol., May 1, 2008; 197(2): 189 - 204. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Watt, B. J. W. van Denderen, L. A. Castelli, C. R. Bruce, A. J. Hoy, E. W. Kraegen, L. Macaulay, and B. E. Kemp Adipose Triglyceride Lipase Regulation of Skeletal Muscle Lipid Metabolism and Insulin Responsiveness Mol. Endocrinol., May 1, 2008; 22(5): 1200 - 1212. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. A. Ducharme and P. E. Bickel Minireview: Lipid Droplets in Lipogenesis and Lipolysis Endocrinology, March 1, 2008; 149(3): 942 - 949. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. C. Kienesberger, A. Lass, K. Preiss-Landl, H. Wolinski, S. D. Kohlwein, R. Zimmermann, and R. Zechner Identification of an Insulin-regulated Lysophospholipase with Homology to Neuropathy Target Esterase J. Biol. Chem., February 29, 2008; 283(9): 5908 - 5917. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. E. Perrone, D. A. L. Mattocks, G. Hristopoulos, J. D. Plummer, R. A. Krajcik, and N. Orentreich Methionine restriction effects on 11 -HSD1 activity and lipogenic/lipolytic balance in F344 rat adipose tissue J. Lipid Res., January 1, 2008; 49(1): 12 - 23. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Holness Leptin: A Central Role in an Expanding Answer to Weight Loss Endocrinology, December 1, 2007; 148(12): 5601 - 5603. [Full Text] [PDF]
|
|
|
|
|
|
L. L. Listenberger, A. G. Ostermeyer-Fay, E. B. Goldberg, W. J. Brown, and D. A. Brown Adipocyte differentiation-related protein reduces the lipid droplet association of adipose triglyceride lipase and slows triacylglycerol turnover J. Lipid Res., December 1, 2007; 48(12): 2751 - 2761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Brasaemle Thematic review series: Adipocyte Biology. The perilipin family of structural lipid droplet proteins: stabilization of lipid droplets and control of lipolysis J. Lipid Res., December 1, 2007; 48(12): 2547 - 2559. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Gallardo, E. Bonzon-Kulichenko, T. Fernandez-Agullo, E. Molto, S. Gomez-Alonso, P. Blanco, J. M. Carrascosa, M. Ros, and A. Andres Tissue-Specific Effects of Central Leptin on the Expression of Genes Involved in Lipid Metabolism in Liver and White Adipose Tissue Endocrinology, December 1, 2007; 148(12): 5604 - 5610. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. E. Kershaw, M. Schupp, H.-P. Guan, N. P. Gardner, M. A. Lazar, and J. S. Flier PPAR{gamma} regulates adipose triglyceride lipase in adipocytes in vitro and in vivo Am J Physiol Endocrinol Metab, December 1, 2007; 293(6): E1736 - E1745. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Steinberg, B. E. Kemp, and M. J. Watt Adipocyte triglyceride lipase expression in human obesity Am J Physiol Endocrinol Metab, October 1, 2007; 293(4): E958 - E964. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Bartness and C. K. Song Thematic review series: Adipocyte Biology. Sympathetic and sensory innervation of white adipose tissue J. Lipid Res., August 1, 2007; 48(8): 1655 - 1672. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Jaworski, E. Sarkadi-Nagy, R. E. Duncan, M. Ahmadian, and H. S. Sul Regulation of Triglyceride Metabolism. * IV. Hormonal regulation of lipolysis in adipose tissue Am J Physiol Gastrointest Liver Physiol, July 1, 2007; 293(1): G1 - G4. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. E. Jocken, D. Langin, E. Smit, W. H. M. Saris, C. Valle, G. B. Hul, C. Holm, P. Arner, and E. E. Blaak Adipose Triglyceride Lipase and Hormone-Sensitive Lipase Protein Expression Is Decreased in the Obese Insulin-Resistant State J. Clin. Endocrinol. Metab., June 1, 2007; 92(6): 2292 - 2299. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ryden, J. Jocken, V. van Harmelen, A. Dicker, J. Hoffstedt, M. Wiren, L. Blomqvist, A. Mairal, D. Langin, E. Blaak, et al. Comparative studies of the role of hormone-sensitive lipase and adipose triglyceride lipase in human fat cell lipolysis Am J Physiol Endocrinol Metab, June 1, 2007; 292(6): E1847 - E1855. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yamaguchi, N. Omatsu, E. Morimoto, H. Nakashima, K. Ueno, T. Tanaka, K. Satouchi, F. Hirose, and T. Osumi CGI-58 facilitates lipolysis on lipid droplets but is not involved in the vesiculation of lipid droplets caused by hormonal stimulation J. Lipid Res., May 1, 2007; 48(5): 1078 - 1089. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Berthiaume, M. Laplante, W. Festuccia, Y. Gelinas, S. Poulin, J. Lalonde, D. R. Joanisse, R. Thieringer, and Y. Deshaies Depot-Specific Modulation of Rat Intraabdominal Adipose Tissue Lipid Metabolism by Pharmacological Inhibition of 11{beta}-Hydroxysteroid Dehydrogenase Type 1 Endocrinology, May 1, 2007; 148(5): 2391 - 2397. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Wei, W. Gao, and R. Lehner Attenuation of Adipocyte Triacylglycerol Hydrolase Activity Decreases Basal Fatty Acid Efflux J. Biol. Chem., March 16, 2007; 282(11): 8027 - 8035. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Granneman, H.-P. H. Moore, R. L. Granneman, A. S. Greenberg, M. S. Obin, and Z. Zhu Analysis of Lipolytic Protein Trafficking and Interactions in Adipocytes J. Biol. Chem., February 23, 2007; 282(8): 5726 - 5735. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Schweiger, R. Schreiber, G. Haemmerle, A. Lass, C. Fledelius, P. Jacobsen, H. Tornqvist, R. Zechner, and R. Zimmermann Adipose Triglyceride Lipase and Hormone-sensitive Lipase Are the Major Enzymes in Adipose Tissue Triacylglycerol Catabolism J. Biol. Chem., December 29, 2006; 281(52): 40236 - 40241. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Notari, V. Baladron, J. D. Aroca-Aguilar, N. Balko, R. Heredia, C. Meyer, P. M. Notario, S. Saravanamuthu, M.-L. Nueda, F. Sanchez-Sanchez, et al. Identification of a Lipase-linked Cell Membrane Receptor for Pigment Epithelium-derived Factor J. Biol. Chem., December 8, 2006; 281(49): 38022 - 38037. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Fex, S. Lucas, M. S. Winzell, B. Ahren, C. Holm, and H. Mulder {beta}-Cell Lipases and Insulin Secretion Diabetes, December 1, 2006; 55(Supplement_2): S24 - S31. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Moldes, G. Beauregard, M. Faraj, N. Peretti, P.-H. Ducluzeau, M. Laville, R. Rabasa-Lhoret, H. Vidal, and K. Clement Adiponutrin gene is regulated by insulin and glucose in human adipose tissue Eur. J. Endocrinol., September 1, 2006; 155(3): 461 - 468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Wilson, S. D. Gardner, N. M. Lambie, S. A. Commans, and D. J. Crowther Characterization of the human patatin-like phospholipase family J. Lipid Res., September 1, 2006; 47(9): 1940 - 1949. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Okazaki, M. Igarashi, M. Nishi, M. Tajima, M. Sekiya, S. Okazaki, N. Yahagi, K. Ohashi, K. Tsukamoto, M. Amemiya-Kudo, et al. Identification of a novel member of the carboxylesterase family that hydrolyzes triacylglycerol: a potential role in adipocyte lipolysis. Diabetes, July 1, 2006; 55(7): 2091 - 2097. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Y. Kim, K. Tillison, J.-H. Lee, D. A. Rearick, and C. M. Smas The adipose tissue triglyceride lipase ATGL/PNPLA2 is downregulated by insulin and TNF-{alpha} in 3T3-L1 adipocytes and is a target for transactivation by PPAR{gamma} Am J Physiol Endocrinol Metab, July 1, 2006; 291(1): E115 - E127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Haemmerle, A. Lass, R. Zimmermann, G. Gorkiewicz, C. Meyer, J. Rozman, G. Heldmaier, R. Maier, C. Theussl, S. Eder, et al. Defective lipolysis and altered energy metabolism in mice lacking adipose triglyceride lipase. Science, May 5, 2006; 312(5774): 734 - 737. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Schoenborn, I. M. Heid, C. Vollmert, A. Lingenhel, T. D. Adams, P. N. Hopkins, T. Illig, R. Zimmermann, R. Zechner, S. C. Hunt, et al. The ATGL Gene Is Associated With Free Fatty Acids, Triglycerides, and Type 2 Diabetes. Diabetes, May 1, 2006; 55(5): 1270 - 1275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Marcinkiewicz, D. Gauthier, A. Garcia, and D. L. Brasaemle The Phosphorylation of Serine 492 of Perilipin A Directs Lipid Droplet Fragmentation and Dispersion J. Biol. Chem., April 28, 2006; 281(17): 11901 - 11909. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. E. Johansson, J. Hoffstedt, H. Parikh, E. Carlsson, M. Wabitsch, A.-G. Bondeson, J. Hedenbro, H. Tornqvist, L. Groop, and M. Ridderstrale Variation in the Adiponutrin Gene Influences Its Expression and Associates With Obesity Diabetes, March 1, 2006; 55(3): 826 - 833. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-H. Lee, K. Kang, I. R. Mehl, R. Nofsinger, W. A. Alaynick, L.-W. Chong, J. M. Rosenfeld, and R. M. Evans Peroxisome proliferator-activated receptor {delta} promotes very low-density lipoprotein-derived fatty acid catabolism in the macrophage PNAS, February 14, 2006; 103(7): 2434 - 2439. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mandard, F. Zandbergen, E. van Straten, W. Wahli, F. Kuipers, M. Muller, and S. Kersten The Fasting-induced Adipose Factor/Angiopoietin-like Protein 4 Is Physically Associated with Lipoproteins and Governs Plasma Lipid Levels and Adiposity J. Biol. Chem., January 13, 2006; 281(2): 934 - 944. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. F. Kurat, K. Natter, J. Petschnigg, H. Wolinski, K. Scheuringer, H. Scholz, R. Zimmermann, R. Leber, R. Zechner, and S. D. Kohlwein Obese Yeast: Triglyceride Lipolysis Is Functionally Conserved from Mammals to Yeast J. Biol. Chem., January 6, 2006; 281(1): 491 - 500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. E. Kershaw, J. K. Hamm, L. A.W. Verhagen, O. Peroni, M. Katic, and J. S. Flier Adipose Triglyceride Lipase: Function, Regulation by Insulin, and Comparison With Adiponutrin Diabetes, January 1, 2006; 55(1): 148 - 157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-P. H. Moore, R. B. Silver, E. P. Mottillo, D. A. Bernlohr, and J. G. Granneman Perilipin Targets a Novel Pool of Lipid Droplets for Lipolytic Attack by Hormone-sensitive Lipase J. Biol. Chem., December 30, 2005; 280(52): 43109 - 43120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Chen, L. Liu, Y. Zhang, H. N. Ginsberg, and Y.-H. Yu Whole-body Insulin Resistance in the Absence of Obesity in FVB Mice With Overexpression of Dgat1 in Adipose Tissue Diabetes, December 1, 2005; 54(12): 3379 - 3386. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Lake, Y. Sun, J.-L. Li, J. E. Kim, J. W. Johnson, D. Li, T. Revett, H. H. Shih, W. Liu, J. E. Paulsen, et al. Expression, regulation, and triglyceride hydrolase activity of Adiponutrin family members J. Lipid Res., November 1, 2005; 46(11): 2477 - 2487. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Langin, A. Dicker, G. Tavernier, J. Hoffstedt, A. Mairal, M. Ryden, E. Arner, A. Sicard, C. M. Jenkins, N. Viguerie, et al. Adipocyte Lipases and Defect of Lipolysis in Human Obesity Diabetes, November 1, 2005; 54(11): 3190 - 3197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chung, J. M. Brown, M. B. Sandberg, and M. McIntosh Trans-10,cis-12 CLA increases adipocyte lipolysis and alters lipid droplet-associated proteins: role of mTOR and ERK signaling J. Lipid Res., May 1, 2005; 46(5): 885 - 895. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Petry, Y. B. Ali, H. Chahinian, H. Jordan, H. Kleine, G. Muller, F. Carriere, and A. Abousalham Sensitive assay for hormone-sensitive lipase using NBD-labeled monoacylglycerol to detect low activities in rat adipocytes J. Lipid Res., March 1, 2005; 46(3): 603 - 614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zimmermann, J. G. Strauss, G. Haemmerle, G. Schoiswohl, R. Birner-Gruenberger, M. Riederer, A. Lass, G. Neuberger, F. Eisenhaber, A. Hermetter, et al. Fat Mobilization in Adipose Tissue Is Promoted by Adipose Triglyceride Lipase Science, November 19, 2004; 306(5700): 1383 - 1386. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
