HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 45, 47242-47253, November 5, 2004

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 279/45/47242    most recent M406644200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Breuza, L. Articles by Hauri, H.-P. Search for Related Content PubMed PubMed Citation Articles by Breuza, L. Articles by Hauri, H.-P. Social Bookmarking                      What's this?

Proteomics of Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC) Membranes from Brefeldin A-treated HepG2 Cells Identifies ERGIC-32, a New Cycling Protein That Interacts with Human Erv46^*

Lionel Breuza, Regula Halbeisen, Paul Jenö, Stefan Otte¶, Charles Barlowe, Wanjin Hong||, and Hans-Peter Hauri**

From the Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland, the Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire, and the ^||Institute of Molecular and Cell Biology, Singapore 117609, Singapore

Cycling proteins play important roles in the organization and function of the early secretory pathway by participating in membrane traffic and selective transport of cargo between the endoplasmic reticulum (ER), the intermediate compartment (ERGIC), and the Golgi. To identify new cycling proteins, we have developed a novel procedure for the purification of ERGIC membranes from HepG2 cells treated with brefeldin A, a drug known to accumulate cycling proteins in the ERGIC. Membranes enriched 110-fold over the homogenate for ERGIC-53 were obtained and analyzed by mass spectrometry. Major proteins corresponded to established and putative cargo receptors and components mediating protein maturation and membrane traffic. Among the uncharacterized proteins, a 32-kDa protein termed ERGIC-32 is a novel cycling membrane protein with sequence homology to Erv41p and Erv46p, two proteins enriched in COPII vesicles of yeast. ERGIC-32 localizes to the ERGIC and partially colocalizes with the human homologs of Erv41p and Erv46p, which mainly localize to the cis-Golgi. ERGIC-32 interacts with human Erv46 (hErv46) as revealed by covalent cross-linking and mistargeting experiments, and silencing of ERGIC-32 by small interfering RNAs increases the turnover of hErv46. We propose that ERGIC-32 functions as a modulator of the hErv41-hErv46 complex by stabilizing hErv46. Our novel approach for the isolation of the ERGIC from BFA-treated cells may ultimately lead to the identification of all proteins rapidly cycling early in the secretory pathway.

Received for publication, June 15, 2004 , and in revised form, August 10, 2004.

The nucleotide sequence reported in this paper has been submitted to the Swiss Protein Database under Swiss-Prot accession no. Q969X5.

^* This work was supported by the Swiss National Science Foundation and the University of Basel. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplementary Fig. 1.

^¶ Current address: Dept. of Biochemistry and Molecular Genetics, University of Illinois, Chicago, IL 60607.

^** To whom correspondence should be addressed. Tel.: 41-61-267-2222 or -2229; Fax: 41-61-267-2208; E-mail: Hans-Peter.Hauri{at}unibas.ch.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				D. Carrel, J. Masson, S. Al Awabdh, C. B. Capra, Z. Lenkei, M. Hamon, M. B. Emerit, and M. Darmon Targeting of the 5-HT1A Serotonin Receptor to Neuronal Dendrites Is Mediated by Yif1B J. Neurosci., August 6, 2008; 28(32): 8063 - 8073. [Abstract] [Full Text] [PDF]

				B. W. Neuman, J. S. Joseph, K. S. Saikatendu, P. Serrano, A. Chatterjee, M. A. Johnson, L. Liao, J. P. Klaus, J. R. Yates III, K. Wuthrich, et al. Proteomics Analysis Unravels the Functional Repertoire of Coronavirus Nonstructural Protein 3 J. Virol., June 1, 2008; 82(11): 5279 - 5294. [Abstract] [Full Text] [PDF]

				S. Mitrovic, H. Ben-Tekaya, E. Koegler, J. Gruenberg, and H.-P. Hauri The Cargo Receptors Surf4, Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)-53, and p25 Are Required to Maintain the Architecture of ERGIC and Golgi Mol. Biol. Cell, May 1, 2008; 19(5): 1976 - 1990. [Abstract] [Full Text] [PDF]

				H. Zheng, J. McKay, and J. E. Buss H-Ras Does Not Need COP I- or COP II-dependent Vesicular Transport to Reach the Plasma Membrane J. Biol. Chem., August 31, 2007; 282(35): 25760 - 25768. [Abstract] [Full Text] [PDF]

				M. Hosaka, T. Watanabe, Y. Yamauchi, Y. Sakai, M. Suda, S. Mizutani, T. Takeuchi, T. Isobe, and T. Izumi A Subset of p23 Localized on Secretory Granules in Pancreatic {beta}-cells J. Histochem. Cytochem., March 1, 2007; 55(3): 235 - 245. [Abstract] [Full Text] [PDF]

				L. M. Welsh, A. H. Y. Tong, C. Boone, O. N. Jensen, and S. Otte Genetic and molecular interactions of the Erv41p-Erv46p complex involved in transport between the endoplasmic reticulum and Golgi complex J. Cell Sci., November 15, 2006; 119(22): 4730 - 4740. [Abstract] [Full Text] [PDF]

				J. J. Ladasky, S. Boyle, M. Seth, H. Li, T. Pentcheva, F. Abe, S. J. Steinberg, and M. Edidin Bap31 Enhances the Endoplasmic Reticulum Export and Quality Control of Human Class I MHC Molecules J. Immunol., November 1, 2006; 177(9): 6172 - 6181. [Abstract] [Full Text] [PDF]

				C. Appenzeller-Herzog and H.-P. Hauri The ER-Golgi intermediate compartment (ERGIC): in search of its identity and function J. Cell Sci., June 1, 2006; 119(11): 2173 - 2183. [Abstract] [Full Text] [PDF]

				E. Hodges, J. S. Redelius, W. Wu, and C. Hoog Accelerated Discovery of Novel Protein Function in Cultured Human Cells Mol. Cell. Proteomics, September 1, 2005; 4(9): 1319 - 1327. [Abstract] [Full Text] [PDF]