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J. Biol. Chem., Vol. 279, Issue 46, 47740-47745, November 12, 2004

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MLE Functions as a Transcriptional Regulator of the roX2 Gene^*

Chee-Gun Lee, Trevor W. Reichman, Tina Baik, and Michael B. Mathews

From the Department of Biochemistry and Molecular Biology, Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103

Dosage compensation is a process that equalizes transcription activity between the sexes. In Drosophila, two non-coding RNA, roX1 and roX2, and at least six protein regulators, MSL-1, MSL-2, MSL-3, MLE, MOF, and JIL-1, have been identified as essential for dosage compensation. Although there is accumulating evidence of the intricate functional and physical interactions between protein and RNA regulators, little is known about how roX RNA expression and function are modulated in coordination with protein regulators. In this report, we have found that a relatively short (about 350 bp) upstream genomic region of the roX2 gene, Prox2, harbors an activity that drives transcription of the downstream gene. Our study has shown that MLE can stimulate the transcription activity of Prox2 and that MLE associates with Prox2 through direct interaction with a newly identified 54-bp repeat, Prox. Our observations suggest a novel mechanism by which roX2 RNA is regulated at the transcriptional level.

Received for publication, July 20, 2004 , and in revised form, September 8, 2004.

^* This work was supported by a grant from the University of Medicine and Dentistry of New Jersey Foundation (to C.-G. L.) and by National Institutes of Health Grant AI34552 (to M. B. M.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed. Tel.: 973-972-0130; Fax: 973-972-5594; E-mail: leecg{at}umdnj.edu.

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