HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 47, 49355-49366, November 19, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/47/49355    most recent M401287200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Soulet, D. Articles by Poulin, R. Search for Related Content PubMed PubMed Citation Articles by Soulet, D. Articles by Poulin, R. Social Bookmarking                      What's this?

A Fluorescent Probe of Polyamine Transport Accumulates into Intracellular Acidic Vesicles via a Two-step Mechanism^*

Denis Soulet, Bruno Gagnon, Serge Rivest, Marie Audette¶||, and Richard Poulin**

From the Oncological and Molecular Endocrinology Research Center, CHUL Research Center, Sainte Foy, Quebec G1V 4G2, Canada and the Departments of Anatomy and Physiology and ^¶Medical Biology, Faculty of Medicine, Laval University, Quebec G1K 7P4, Canada

Mammalian polyamine carriers have not yet been molecularly identified. The fluoroprobe Spd-C₂-BODIPY faithfully reports polyamine transport and accumulates almost exclusively in polyamine-sequestering vesicles (PSVs). Polyamines might thus be imported first by a plasma membrane carrier and then sequestered into pre-existing PSVs (model A), or be directly captured by polyamine receptors undergoing endocytosis (model B). Spd-C₂-BODIPY uptake was unaffected in receptor-mediated endocytosis-deficient Chinese hamster ovary cell mutants. PSVs strongly colocalized with acidic vesicles of the late endocytic compartment and the trans Golgi. Virtually perfect colocalization between PSVs and acidic vesicles was found in Chinese hamster ovary cell mutants that are blocked either in the late endosome/lysosome fusion process or in the maturation of multivesicular bodies. Prior inhibition of the V-ATPase dramatically decreased total Spd-C₂-BODIPY accumulation while increasing cytosolic fluorescence. Conversely, cells pre-loaded with the probe slowly released it from PSVs upon V-ATPase inhibition. The present data thus support model A, and indicate that polyamine accumulation is primarily driven by the activity of a vesicular H⁺:polyamine carrier.

Received for publication, February 5, 2004 , and in revised form, June 14, 2004.

^* This work was supported in part by Grant IC072641 from the Canadian Institutes of Health Research (to R. P. and M. A.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^|| Holds a Bourse de Chercheur National des Fonds de Recherche en Santé du Québec (FRSQ).

^** To whom correspondence should be addressed: Oncological and Molecular Endocrinology Research Center, CHUL Research Center, 2705 Laurier Blvd., Ste. Foy, Quebec G1V 4G2, Canada. Tel.: 418-654-2296; Fax: 418-654-2761; E-mail: Richard.Poulin{at}crchul.ulaval.ca.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C.-P. Liao, M. E. Lasbury, S.-H. Wang, C. Zhang, P. J. Durant, Y. Murakami, S. Matsufuji, and C.-H. Lee Pneumocystis Mediates Overexpression of Antizyme Inhibitor Resulting in Increased Polyamine Levels and Apoptosis in Alveolar Macrophages J. Biol. Chem., March 20, 2009; 284(12): 8174 - 8184. [Abstract] [Full Text] [PDF]

				A. M. Kaufmann and J. P. Krise Niemann-Pick C1 Functions in Regulating Lysosomal Amine Content J. Biol. Chem., September 5, 2008; 283(36): 24584 - 24593. [Abstract] [Full Text] [PDF]

				A. E. Pegg Regulation of Ornithine Decarboxylase J. Biol. Chem., May 26, 2006; 281(21): 14529 - 14532. [Abstract] [Full Text] [PDF]

				K. Hoshino, E. Momiyama, K. Yoshida, K. Nishimura, S. Sakai, T. Toida, K. Kashiwagi, and K. Igarashi Polyamine Transport by Mammalian Cells and Mitochondria: ROLE OF ANTIZYME AND GLYCOSAMINOGLYCANS J. Biol. Chem., December 30, 2005; 280(52): 42801 - 42808. [Abstract] [Full Text] [PDF]