HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 48, 50157-50166, November 26, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/48/50157    most recent M405414200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Woods, Y. L. Articles by Saville, M. K. Search for Related Content PubMed PubMed Citation Articles by Woods, Y. L. Articles by Saville, M. K. Social Bookmarking                      What's this?

p14 Arf Promotes Small Ubiquitin-like Modifier Conjugation of Werners Helicase^*

Yvonne L. Woods¶, Dimitris P. Xirodimas, Alan R. Prescott||, Alison Sparks, David P. Lane**, and Mark K. Saville

From the CR-UK Cell Transformation Research Group, Department of Surgery and Molecular Oncology, Ninewells Hospital and Medical School, Ninewells Avenue, Dundee DD1 9SYand ^||University of Dundee, MSI/WTB Complex, School of Life Sciences, Dow Street, Dundee DD1 5EH, United Kingdom

Here we demonstrate a novel p53-independent interaction between the nucleolar tumor suppressors, p14 Arf and Werners helicase (WRN). Binding of p14 Arf to WRN is multivalent and resembles the binding of p14 Arf to Mdm2. Residues 2–14 and 82–101 of p14 Arf and residues in the central region and C terminus of WRN have particular importance for binding. p14 Arf promotes small ubiquitin-like modifier (SUMO) modification of WRN in a synergistic manner with the SUMO-conjugating enzyme, UBCH9. p14 Arf causes redistribution of WRN within the nucleus, and this effect is reversed by expression of a SUMO-specific protease, thus implicating the SUMO conjugation pathway in WRN re-localization. We establish that the ability to promote SUMO conjugation is a general property of the p14 Arf tumor suppressor.

Received for publication, May 14, 2004 , and in revised form, August 17, 2004.

^* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Recipient of a post-doctoral fellowship from Cancer Research UK.

^** Gibb fellow of Cancer Research UK.

^¶ To whom correspondence should be addressed. Tel.: 01382-496362; Fax: 01382-496361; E-mail: y.l.woods{at}dundee.ac.uk.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				Y. Huang, M. Wu, and H.-Y. Li Tumor Suppressor ARF Promotes Non-classic Proteasome-independent Polyubiquitination of COMMD1 J. Biol. Chem., April 25, 2008; 283(17): 11453 - 11460. [Abstract] [Full Text] [PDF]

				R. M. Brosh Jr and V. A. Bohr Human premature aging, DNA repair and RecQ helicases Nucleic Acids Res., December 3, 2007; 35(22): 7527 - 7544. [Abstract] [Full Text] [PDF]

				B. Xhemalce, E. M. Riising, P. Baumann, A. Dejean, B. Arcangioli, and J.-S. Seeler Role of SUMO in the dynamics of telomere maintenance in fission yeast PNAS, January 16, 2007; 104(3): 893 - 898. [Abstract] [Full Text] [PDF]

				K. Tago, S. Chiocca, and C. J. Sherr Sumoylation induced by the Arf tumor suppressor: A p53-independent function PNAS, May 24, 2005; 102(21): 7689 - 7694. [Abstract] [Full Text] [PDF]

				S. Eladad, T.-Z. Ye, P. Hu, M. Leversha, S. Beresten, M. J. Matunis, and N. A. Ellis Intra-nuclear trafficking of the BLM helicase to DNA damage-induced foci is regulated by SUMO modification Hum. Mol. Genet., May 15, 2005; 14(10): 1351 - 1365. [Abstract] [Full Text] [PDF]

				C.J. SHERR, D. BERTWISTLE, W. DEN BESTEN, M.-L. KUO, M. SUGIMOTO, K. TAGO, R.T. WILLIAMS, F. ZINDY, and M.F. ROUSSEL p53-Dependent and -Independent Functions of the Arf Tumor Suppressor Cold Spring Harb Symp Quant Biol, January 1, 2005; 70(0): 129 - 137. [Abstract] [PDF]