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J. Biol. Chem., Vol. 279, Issue 48, 50302-50309, November 26, 2004

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The La Motif and the RNA Recognition Motifs of Human La Autoantigen Contribute Individually to RNA Recognition and Subcellular Localization^*

Sven Horke, Kerstin Reumann, Christian Schulze, Frank Grosse¶, and Tilman Heise||

From the Heinrich-Pette-Institute for Experimental Virology and Immunology at the University of Hamburg, Martinistrasse 52, 20251 Hamburg, Germany, Center for Molecular Neurobiology, University-Hospital Hamburg, Falkenried 94, 20251 Hamburg, Germany, and ^¶Institute for Molecular Biotechnology, Beutenbergstrasse 11, 07745 Jena, Germany

The human La autoantigen (hLa) protein is a predominantly nuclear phosphoprotein that contains three potential RNA binding domains referred to as the La motif and the RNA recognition motifs RRMs 1 and 2. With this report, we differentiated the contribution of its three RNA binding domains to RNA binding by combining in vitro and in vivo assays. Also, surface plasmon resonance technology was used to generate a model for the sequential contribution of the RNA binding domains to RNA binding. The results indicated that the La motif may contribute to specificity rather than affinity, whereas RRM1 is indispensable for association with pre-tRNA and hY1 RNA. Furthermore, RRM2 was not crucial for the interaction with various RNAs in vivo, although needed for full-affinity binding in vitro. Moreover, earlier studies suggest that RNA binding by hLa may direct its subcellular localization. As shown previously for RRM1, deletion of RNP2 sequence in RRM1 alters nucleolar distribution of hLa, not observed after deletion of the La motif. Here we discuss a model for precursor RNA binding based on a sequential association process mediated by RRM1 and the La motif.

Received for publication, July 6, 2004 , and in revised form, August 18, 2004.

^* This work was supported by the Deutsche Forschungsgemeinschaft HE 2814/2-3 (to T. H.), WI 664/9-1, and the Bundesministerium für Bildung und Forschung (Nationales Genomforschungsnetz and Kompetenznetz Hepatitis). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^|| To whom correspondence should be addressed: Heinrich-Pette-Institute for Experimental Virology and Immunology, University of Hamburg, Postfach 201652, 20251 Hamburg, Germany. Tel.: 49-40-48051-225; Fax: 49-40-48051-222; E-mail heise{at}hpi.uni-hamburg.de.

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