HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 49, 50857-50863, December 3, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/49/50857    most recent M409079200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Davidson, M. K. Articles by Wahls, W. P. Search for Related Content PubMed PubMed Citation Articles by Davidson, M. K. Articles by Wahls, W. P. Social Bookmarking                      What's this?

Atf1-Pcr1-M26 Complex Links Stress-activated MAPK and cAMP-dependent Protein Kinase Pathways via Chromatin Remodeling of cgs2^+*

Mari K. Davidson, Harish K. Shandilya, Kouji Hirota, Kunihiro Ohta, and Wayne P. Wahls¶

From the Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205 and the Genetic Dynamics Research Unit Laboratory, The Institute of Physical and Chemical Research, Wako, Saitama 351-0198, Japan

Although co-ordinate interaction between different signal transduction pathways is essential for developmental decisions, interpathway connections are often obscured and difficult to identify due to cross-talk. Here signals from the fission yeast stress-activated MAPK Spc1 are shown to regulate Cgs2, a negative regulator of the cAMP-dependent protein kinase (protein kinase A) pathway. Pathway integration is achieved via Spc1-dependent binding of Atf1-Pcr1 heterodimer to an M26 DNA site in the cgs2⁺ promoter, which remodels chromatin to regulate expression of cgs2⁺ and targets downstream of protein kinase A. This direct interpathway connection co-ordinates signals of nitrogen and carbon source depletion to affect a G₀ cell-cycle checkpoint and sexual differentiation. The Atf1-Pcr1-M26 complex-dependent chromatin remodeling provides a molecular mechanism whereby Atf1-Pcr1 heterodimer can function differentially as either a transcriptional activator, or as a transcriptional repressor, or as an inducer of meiotic recombination. We also show that the Atf1-Pcr1-M26 complex functions as both an inducer and repressor of chromatin remodeling, which provides a way for various chromatin remodeling-dependent effector functions to be regulated.

Received for publication, August 9, 2004 , and in revised form, September 7, 2004.

^* This study was supported by Grants GM62244 and GM62801 from the NIGMS, National Institutes of Health. Additional support was provided by the Bioarchitect Research Program of The Institute of Physical and Chemical Research and the Core Research for Evolutional Science and Technology of Japan Science and Technology Corporation, and the Ministry of Education, Science, Culture & Sports, Japan. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence should be addressed: Dept. of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, 4301 W. Markham Street (slot 516), Little Rock, AR 72205-7199. Tel.: 501-686-5787; Fax: 501-526-7008; E-mail: wahlswaynep{at}uams.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K. Sajiki, M. Hatanaka, T. Nakamura, K. Takeda, M. Shimanuki, T. Yoshida, Y. Hanyu, T. Hayashi, Y. Nakaseko, and M. Yanagida Genetic control of cellular quiescence in S. pombe J. Cell Sci., May 1, 2009; 122(9): 1418 - 1429. [Abstract] [Full Text] [PDF]

				J. Gao, M. K. Davidson, and W. P. Wahls Distinct regions of ATF/CREB proteins Atf1 and Pcr1 control recombination hotspot ade6-M26 and the osmotic stress response Nucleic Acids Res., May 1, 2008; 36(9): 2838 - 2851. [Abstract] [Full Text] [PDF]

				M. Sanso, M. Gogol, J. Ayte, C. Seidel, and E. Hidalgo Transcription Factors Pcr1 and Atf1 Have Distinct Roles in Stress- and Sty1-Dependent Gene Regulation Eukaryot. Cell, May 1, 2008; 7(5): 826 - 835. [Abstract] [Full Text] [PDF]

				K. Hirota, W. W. Steiner, T. Shibata, and K. Ohta Multiple Modes of Chromatin Configuration at Natural Meiotic Recombination Hot Spots in Fission Yeast Eukaryot. Cell, November 1, 2007; 6(11): 2072 - 2080. [Abstract] [Full Text] [PDF]