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J. Biol. Chem., Vol. 279, Issue 49, 51472-51481, December 3, 2004

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Differential Expression and Interaction with the Visual G-protein Transducin of Centrin Isoforms in Mammalian Photoreceptor Cells^*

Andreas Giessl, Alexander Pulvermüller¶||, Philipp Trojan, Jung Hee Park¶, Hui-Woog Choe¶**, Oliver Peter Ernst¶, Klaus Peter Hofmann¶, and Uwe Wolfrum

From the Institut für Zoologie, Johannes Gutenberg-Universität Mainz, 55099 Mainz, Germany, the ^¶Institut für Medizinische Physik und Biophysik, Charité-Universitätsmedizin Berlin, Schumannstrasse 20-21, 10098 Berlin, Germany, and the ^**Department of Chemistry, College of Natural Science, Chonbuk National University, Chonju 561-756, South Korea

Photoisomerization of rhodopsin activates a heterotrimeric G-protein cascade leading to closure of cGMP-gated channels and hyperpolarization of photoreceptor cells. Massive translocation of the visual G-protein transducin, G_t, between subcellular compartments contributes to long term adaptation of photoreceptor cells. Ca²⁺-triggered assembly of a centrin-transducin complex in the connecting cilium of photoreceptor cells may regulate these transducin translocations. Here we demonstrate expression of all four known, closely related centrin isoforms in the mammalian retina. Interaction assays revealed binding potential of the four centrin isoforms to G_t heterodimers. High affinity binding to G_t and subcellular localization of the centrin isoforms Cen1 and Cen2 in the connecting cilium indicated that these isoforms contribute to the centrin-transducin complex and potentially participate in the regulation of transducin translocation through the photoreceptor cilium. Binding of Cen2 and Cen4 to G of non-visual G-proteins may additionally regulate G-proteins involved in centrosome and basal body functions.

Received for publication, June 17, 2004 , and in revised form, August 13, 2004.

^* This work was supported by Grants Ho832/6 (to A. P., O. P. E., and K. P. H.) and Wo548/3 (to U. W.) from the Deutsche Forschungsgemeinschaft (DFG) priority program SPP 1025 "Molecular Sensory Physiology" and by a grant from the FAUN-Stiftung, Nürnberg, Germany (to U. W.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Both authors contributed equally to this work.

^|| To whom correspondence may be addressed: Inst. für Medizinische Physik und Biophysik, Charité-Universitätsmedizin Berlin, Ziegelstrasse 5-9, D-10117 Berlin, Germany. Tel.: 49-30-450-524176; Fax: 49-30-450-524952; E-mail: alexander.pulvermueller{at}charite.de.

To whom correspondence may be addressed: Inst. für Zoologie, Abt.1, Johannes Gutenberg-Universität Mainz, Müllerweg 6, D-55099 Mainz, Germany. Tel.: 49-6131-39-25148; Fax: 49-6131-39-23815; E-mail: wolfrum{at}mail.uni-mainz.de.

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