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J. Biol. Chem., Vol. 279, Issue 50, 52465-52472, December 10, 2004

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Mutations in the DNA-binding Domain of the Transcription Factor Bright Act as Dominant Negative Proteins and Interfere with Immunoglobulin Transactivation^*

Jamee C. Nixon, Jaya Rajaiya, and Carol F. Webb¶||

From the Oklahoma Medical Research Foundation, 825 N. E. 13^th Street, Oklahoma City, Oklahoma 73104 and the Departments of Microbiology and Immunology and ^¶Cell Biology, Oklahoma University Health Sciences Center, Oklahoma City, Oklahoma 73104

Bright, for B cell regulator of immunoglobulin heavy chain transcription, binds A+T-rich sequences in the intronic enhancer regions of the murine heavy chain locus and 5'-flanking sequences of some variable heavy chain promoters. Most resting B cells do not express Bright; however, it is induced after stimulation with antigen or polyclonal mitogens. Bright activation results in up-regulation of µ transcription; however, it is not clear whether Bright function is critical for normal B cell development. To begin to address Bright function during B cell development, seven mutated forms of Bright were produced. Five of the seven mutants revealed little or no DNA binding activity. Furthermore, because Bright binds DNA as a dimer, two of the mutants formed complexes with wild type Bright and acted in a dominant negative fashion. Dominant negative Bright prevented the up-regulation of µ transcription in transfected Chinese hamster ovary cells transfected with wild type Bright. These data identify regions within Bright that are required for the DNA binding activity of Bright and for its function as a transcription factor.

Received for publication, March 18, 2004 , and in revised form, August 3, 2004.

^* This work was supported by the National Institutes of Health Grants AI45864, AI44215, and T32 07364. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^|| To whom correspondence should be addressed: Oklahoma Medical Research Foundation, Immunobiology and Cancer Research Program, MS#36, 825 N. E. 13th St., Oklahoma City, OK 73104. Tel.: 405-271-7564; Fax: 405-271-7128; E-mail: carol-webb{at}omrf.ouhsc.edu.

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