HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 51, 53886-53891, December 17, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/51/53886    most recent M407708200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Price, M. P. Articles by Benson, C. J. Search for Related Content PubMed PubMed Citation Articles by Price, M. P. Articles by Benson, C. J. Social Bookmarking                      What's this?

Stomatin Modulates Gating of Acid-sensing Ion Channels^*

Margaret P. Price, Robert J. Thompson, Jayasheel O. Eshcol, John A. Wemmie¶, and Christopher J. Benson||

From the Departments of Internal Medicine and ^¶Psychiatry and the Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City, Iowa 52242

Acid-sensing ion channels (ASICs) are H⁺-gated members of the degenerin/epithelial Na⁺ channel (DEG/ENaC) family in vertebrate neurons. Several ASICs are expressed in sensory neurons, where they play a role in responses to nociceptive, taste, and mechanical stimuli; others are expressed in central neurons, where they participate in synaptic plasticity and some forms of learning. Stomatin is an integral membrane protein found in lipid/protein-rich microdomains, and it is believed to regulate the function of ion channels and transporters. In Caenorhabditis elegans, stomatin homologs interact with DEG/ENaC channels, which together are necessary for normal mechanosensation in the worm. Therefore, we asked whether stomatin interacts with and modulates the function of ASICs. We found that stomatin co-immunoprecipitated and co-localized with ASIC proteins in heterologous cells. Moreover, stomatin altered the function of ASIC channels. Stomatin potently reduced acid-evoked currents generated by ASIC3 without changing steady state protein levels or the amount of ASIC3 expressed at the cell surface. In contrast, stomatin accelerated the desensitization rate of ASIC2 and heteromeric ASICs, whereas current amplitude was unaffected. These data suggest that stomatin binds to and alters the gating of ASICs. Our findings indicate that modulation of DEG/ENaC channels by stomatin-like proteins is evolutionarily conserved and may have important implications for mammalian nociception and mechanosensation.

Received for publication, July 9, 2004 , and in revised form, September 20, 2004.

^* This work was supported by NHLBI, National Institutes of Health Grant HL04349 (to C. J. B.) and by a Veterans Affairs Research Career Development Award and the Howard Hughes Medical Institute Biomedical Research Support Program (to J. A. W.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^|| To whom correspondence should be addressed: Dept. of Internal Medicine, 200 Hawkins Dr., E314-1 GH, Iowa City, IA 52242-1009. Tel.: 319-356-7031; Fax: 319-353-6343; E-mail: chris-benson{at}uiowa.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. Martinez-Salgado, A. G. Benckendorff, L.-Y. Chiang, R. Wang, N. Milenkovic, C. Wetzel, J. Hu, C. L. Stucky, M. G. Parra, N. Mohandas, et al. Stomatin and Sensory Neuron Mechanotransduction J Neurophysiol, December 1, 2007; 98(6): 3802 - 3808. [Abstract] [Full Text] [PDF]

				S. Chai, M. Li, J. Lan, Z.-G. Xiong, J. A. Saugstad, and R. P. Simon A Kinase-anchoring Protein 150 and Calcineurin Are Involved in Regulation of Acid-sensing Ion Channels ASIC1a and ASIC2a J. Biol. Chem., August 3, 2007; 282(31): 22668 - 22677. [Abstract] [Full Text] [PDF]

				E. Lingueglia Acid-sensing Ion Channels in Sensory Perception J. Biol. Chem., June 15, 2007; 282(24): 17325 - 17329. [Full Text] [PDF]

				N. L. Jernigan and H. A. Drummond Myogenic vasoconstriction in mouse renal interlobar arteries: role of endogenous beta and {gamma}ENaC Am J Physiol Renal Physiol, December 1, 2006; 291(6): F1184 - F1191. [Abstract] [Full Text] [PDF]

				B. T. Carroll, G. R. Dubyak, M. M. Sedensky, and P. G. Morgan Sulfated Signal from ASJ Sensory Neurons Modulates Stomatin-dependent Coordination in Caenorhabditis elegans J. Biol. Chem., November 24, 2006; 281(47): 35989 - 35996. [Abstract] [Full Text] [PDF]

				G. L. Fawcett, C. M. Santi, A. Butler, T. Harris, M. Covarrubias, and L. Salkoff Mutant Analysis of the Shal (Kv4) Voltage-gated Fast Transient K+ Channel in Caenorhabditis elegans J. Biol. Chem., October 13, 2006; 281(41): 30725 - 30735. [Abstract] [Full Text] [PDF]

				A. Davies, L. Douglas, J. Hendrich, J. Wratten, A. Tran Van Minh, I. Foucault, D. Koch, W. S. Pratt, H. R. Saibil, and A. C. Dolphin The Calcium Channel {alpha}2{delta}-2 Subunit Partitions with CaV2.1 into Lipid Rafts in Cerebellum: Implications for Localization and Function. J. Neurosci., August 23, 2006; 26(34): 8748 - 8757. [Abstract] [Full Text] [PDF]

				W. H. Vila-Carriles, G. G. Kovacs, B. Jovov, Z.-H. Zhou, A. K. Pahwa, G. Colby, O. Esimai, G. Y. Gillespie, T. B. Mapstone, J. M. Markert, et al. Surface Expression of ASIC2 Inhibits the Amiloride-sensitive Current and Migration of Glioma Cells J. Biol. Chem., July 14, 2006; 281(28): 19220 - 19232. [Abstract] [Full Text] [PDF]

				E. Deval, V. Friend, C. Thirant, M. Salinas, M. Jodar, M. Lazdunski, and E. Lingueglia Regulation of Sensory Neuron-specific Acid-sensing Ion Channel 3 by the Adaptor Protein Na+/H+ Exchanger Regulatory Factor-1 J. Biol. Chem., January 20, 2006; 281(3): 1796 - 1807. [Abstract] [Full Text] [PDF]

				L. G. Frigeri, T. R. Radabaugh, P. A. Haynes, and M. Hildebrand Identification of Proteins from a Cell Wall Fraction of the Diatom Thalassiosira pseudonana: Insights into Silica Structure Formation Mol. Cell. Proteomics, January 1, 2006; 5(1): 182 - 193. [Abstract] [Full Text] [PDF]

				E. Donier, F. Rugiero, K. Okuse, and J. N. Wood Annexin II Light Chain p11 Promotes Functional Expression of Acid-sensing Ion Channel ASIC1a J. Biol. Chem., November 18, 2005; 280(46): 38666 - 38672. [Abstract] [Full Text] [PDF]