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J. Biol. Chem., Vol. 279, Issue 53, 55895-55904, December 31, 2004

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Biochemical and Cell Biological Analyses of a Mammalian Septin Complex, Sept7/9b/11^*

Koh-ichi Nagata¶, Tomiko Asano, Yoshinori Nozawa||, and Masaki Inagaki

From the Department of Molecular Neurobiology, the Institute for Developmental Research, Aichi Human Service Center, 713-8 Kamiya-Cho, Kasugai, Aichi 480-0392, Japan, the Division of Biochemistry, Aichi Cancer Center Research Institute, 1-1 Kanokoden, Chikusa, Nagoya 464-8681, ^||Gifu International Institute of Biotechnology, 1-1 Nakafudogaoka, Gifu 504-0838, Japan

Septins are members of a conserved family of cytoskeletal GTPases present in organisms as diverse as yeast and mammals. Unlike lower eukaryotic cells, the physiological significance of mammalian septin complexes is largely unknown. Using specific antibodies, we found at least five septins, Sept2, Sept7, Sept8, Sept9b, and Sept11, in septin complexes affinity-purified with anti-Sept7 antibody-conjugated column from rat embryonic fibroblast REF52 cells. Immunofluorescence studies revealed co-localization of Sept7, Sept9b, and Sept11 along stress fibers in REF52 cells. Biochemical and immunoprecipitation analyses revealed that the three septins directly bind with each other through their N- or C-terminal divergent regions. These septins per se formed distinct and characteristic filament structures when transiently expressed in COS7 cells. When two of the three septins were co-expressed in COS7 cells, combination-dependent filament elongation, bundling, or disruption was observed. Taken together, our results suggest that septin filament structures may be affected by interactions with other septins included in the complex.

Received for publication, June 2, 2004 , and in revised form, October 7, 2004.

^* This work was supported by grants-in-aid for scientific research and for cancer research from the Ministry of Education, Science, Technology, Sports, and Culture of Japan, by a grant-in-aid for the Second Term Comprehensive 10-Year Strategy for Cancer Control from the Ministry of Health, Labour, and Welfare, Japan, and by a grant from Yamanouchi Foundation for Research on Metabolic Disorders. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence should be addressed: Dept. of Molecular Neurobiology, The Institute for Developmental Research, Aichi Human Service Center, 713-8 Kamiya-Cho, Kasugai, Aichi 480-0392, Japan. Tel.: 81-568-88-0811 (ext. 3595); Fax: 81-568-88-0829; E-mail: knagata{at}inst-hsc.jp.

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