|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 279, Issue 6, 5025-5034, February 6, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Interplay between Estrogen Response Element Sequence and Ligands Controls in Vivo Binding of Estrogen Receptor to Regulated Genes*
From the Department of Biochemistry, University of Illinois, Urbana, Illinois 61801-3602
To examine the role of the estrogen response element (ERE) sequence in binding of liganded estrogen receptor (ER) to promoters, we analyzed in vivo interaction of liganded ER with the imperfect ERE in the pS2 gene and the composite estrogen-responsive unit (ERU) in the proteinase inhibitor 9 (PI-9) gene. In transient transfections of ER-positive HepG2-ER7 cells, PI-9 was strongly induced by estrogen, moxestrol (MOX), and 4-hydroxytamoxifen (OHT). PI-9 was not induced by raloxifene or ICI 182,780. Quantitative reverse transcriptase-PCR showed that moxestrol strongly induced cellular PI-9 and pS2 mRNAs, whereas OHT moderately induced PI-9 mRNA and weakly induced pS2 mRNA. Chromatin immunoprecipitation experiments demonstrated strong and similar association of 17
-estradiol-hER
and MOX-hER with the PI-9 ERU and with the pS2 ERE. Binding of MOX-hER to the PI-9 ERU and the pS2 ERE was rapid and continuous. Although MOX-hER bound strongly to the PI-9 ERU and less well to the pS2 ERE in chromatin immunoprecipitation, gel shift assays showed that estrogen-hER binds with higher affinity to the deproteinized pS2 ERE than to the PI-9 ERU. Across a broad range of OHT concentrations, OHT-hER associated strongly with the pS2 ERE and weakly with the PI-9 ERU. ICI-hER bound poorly to the PI-9 ERU and effectively to the pS2 ERE. Raloxifene-hER and MOX-hER exhibited similar binding to the PI-9 ERU and the pS2 ERE. These studies demonstrate that ER ligand and ERE sequence work together to regulate in vivo binding of ER to estrogen-responsive promoters.
Received for publication, July 2, 2003 , and in revised form, November 13, 2003.
* This research was supported by NICHD, National Institutes of Health, Grant HD-16720. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Present address: Center for Clinical Sciences Research, Dept. of Radiation Oncology, Stanford University, Stanford, CA 94303-5152.
Present address: Stanford University Hospital, Dept. of Gynecology and Obstetrics, Rm. HH-333, Stanford, CA 94305-5152.
¶ To whom correspondence should be addressed: Dept. of Biochemistry, University of Illinois, 600 S. Mathews Ave., Urbana, IL 61801-3602. Tel.: 217-333-1788; Fax: 217-244-5858; E-mail: djshapir{at}uiuc.edu.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  H.-S. Han, E. Yu, J.-Y. Song, J.-Y. Park, S. J. Jang, and J. Choi The Estrogen Receptor {alpha} Pathway Induces Oncogenic Wip1 Phosphatase Gene Expression Mol. Cancer Res., May 1, 2009; 7(5): 713 - 723. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Jiang, N. M. Patterson, Y. Ling, J. Xie, W. G. Helferich, and D. J. Shapiro Low Concentrations of the Soy Phytoestrogen Genistein Induce Proteinase Inhibitor 9 and Block Killing of Breast Cancer Cells by Immune Cells Endocrinology, November 1, 2008; 149(11): 5366 - 5373. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Mao, N. M. Patterson, M. T. Cherian, I. O. Aninye, C. Zhang, J. B. Montoya, J. Cheng, K. S. Putt, P. J. Hergenrother, E. M. Wilson, et al. A New Small Molecule Inhibitor of Estrogen Receptor {alpha} Binding to Estrogen Response Elements Blocks Estrogen-dependent Growth of Cancer Cells J. Biol. Chem., May 9, 2008; 283(19): 12819 - 12830. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Khan, F. Wu, S. Liu, Q. Wu, and S. Safe Role of specificity protein transcription factors in estrogen-induced gene expression in MCF-7 breast cancer cells J. Mol. Endocrinol., October 1, 2007; 39(4): 289 - 304. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Cheng, C. Zhang, and D. J. Shapiro A Functional Serine 118 Phosphorylation Site in Estrogen Receptor-{alpha} Is Required for Down-Regulation of Gene Expression by 17{beta}-Estradiol and 4-Hydroxytamoxifen Endocrinology, October 1, 2007; 148(10): 4634 - 4641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Kundu, A. Alioua, E. Stefani, and L. Toro Regulation of Mouse Slo Gene Expression: MULTIPLE PROMOTERS, TRANSCRIPTION START SITES, AND GENOMIC ACTION OF ESTROGEN J. Biol. Chem., September 14, 2007; 282(37): 27478 - 27492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Cascio, V. Bartella, C. Garofalo, A. Russo, A. Giordano, and E. Surmacz Insulin-like Growth Factor 1 Differentially Regulates Estrogen Receptor-dependent Transcription at Estrogen Response Element and AP-1 Sites in Breast Cancer Cells J. Biol. Chem., February 9, 2007; 282(6): 3498 - 3506. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wang, C. Zhang, S. K. Nordeen, and D. J. Shapiro In Vitro Fluorescence Anisotropy Analysis of the Interaction of Full-length SRC1a with Estrogen Receptors {alpha} and beta Supports an Active Displacement Model for Coregulator Utilization J. Biol. Chem., February 2, 2007; 282(5): 2765 - 2775. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Krieg, E. M. Hammond, and A. J. Giaccia Functional Analysis of p53 Binding under Differential Stresses. Mol. Cell. Biol., October 1, 2006; 26(19): 7030 - 7045. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Khan, R. Barhoumi, R. Burghardt, S. Liu, K. Kim, and S. Safe Molecular Mechanism of Inhibitory Aryl Hydrocarbon Receptor--Estrogen Receptor/Sp1 Cross Talk in Breast Cancer Cells Mol. Endocrinol., September 1, 2006; 20(9): 2199 - 2214. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Higgins, S. Liu, M. Abdelrahim, K. Yoon, K. Vanderlaag, W. Porter, R. P. Metz, and S. Safe Vascular Endothelial Growth Factor Receptor-2 Expression Is Induced by 17{beta}-Estradiol in ZR-75 Breast Cancer Cells by Estrogen Receptor {alpha}/Sp Proteins Endocrinology, July 1, 2006; 147(7): 3285 - 3295. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Hammond, D. J. Mandell, A. Salim, A. J. Krieg, T. M. Johnson, H. A. Shirazi, L. D. Attardi, and A. J. Giaccia Genome-Wide Analysis of p53 under Hypoxic Conditions. Mol. Cell. Biol., May 1, 2006; 26(9): 3492 - 3504. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Jiang, B. A. Orr, D. M. Kranz, and D. J. Shapiro Estrogen Induction of the Granzyme B Inhibitor, Proteinase Inhibitor 9, Protects Cells against Apoptosis Mediated by Cytotoxic T Lymphocytes and Natural Killer Cells Endocrinology, March 1, 2006; 147(3): 1419 - 1426. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-C. Lin, Y.-L. Tsai, M.-T. Huang, Y.-P. Lu, C.-T. Ho, S.-F. Tseng, and S.-C. Teng Inhibition of estradiol-induced mammary proliferation by dibenzoylmethane through the E2-ER-ERE-dependent pathway Carcinogenesis, January 1, 2006; 27(1): 131 - 136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S K Nair, T J Thomas, N J Greenfield, A Chen, H He, and T Thomas Conformational dynamics of estrogen receptors {alpha} and {beta} as revealed by intrinsic tryptophan fluorescence and circular dichroism J. Mol. Endocrinol., October 1, 2005; 35(2): 211 - 223. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Matthews, B. Wihlen, J. Thomsen, and J.-A. Gustafsson Aryl Hydrocarbon Receptor-Mediated Transcription: Ligand-Dependent Recruitment of Estrogen Receptor {alpha} to 2,3,7,8-Tetrachlorodibenzo- p-Dioxin-Responsive Promoters Mol. Cell. Biol., July 1, 2005; 25(13): 5317 - 5328. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C M Klinge, S C Jernigan, K A Mattingly, K E Risinger, and J Zhang Estrogen response element-dependent regulation of transcriptional activation of estrogen receptors {alpha} and {beta} by coactivators and corepressors J. Mol. Endocrinol., October 1, 2004; 33(2): 387 - 410. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |