HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 8, 6296-6304, February 20, 2004

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 279/8/6296    most recent M310528200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Elm, C. Articles by Hammerschmidt, S. Search for Related Content PubMed PubMed Citation Articles by Elm, C. Articles by Hammerschmidt, S. Social Bookmarking                      What's this?

Ectodomains 3 and 4 of Human Polymeric Immunoglobulin Receptor (hpIgR) Mediate Invasion of Streptococcus pneumoniae into the Epithelium^*

Christine Elm, Ranveig Braathen¶, Simone Bergmann||, Ronald Frank, Jean-Pierre Vaerman**, Charlotte S. Kaetzel, Gursharan S. Chhatwal, Finn-Eirik Johansen¶, and Sven Hammerschmidt||

From the GBF-German Research Centre for Biotechnology, Braunschweig 38124, Germany, the ^¶Institute of Pathology, Laboratory for Immunohistochemistry and Immunopathology, University of Oslo, Rikshospitalet, Oslo 0027, Norway, the ^||Research Center for Infectious Diseases, University of Würzburg, Würzburg 97070, Germany, the ^**Experimental Medicine Unit, ICP, Université Catholique de Louvain, Brussels 1200, Belgium, the Department of Pathology and Laboratory Medicine, University of Kentucky, Lexington Kentucky 40536-0298

Streptococcus pneumoniae binds to the ectodomain of the human polymeric Ig receptor (pIgR), also known as secretory component (SC), via a hexapeptide motif in the choline-binding protein SpsA. The SpsA-pIgR interaction mediates adherence and internalization of the human pathogen into epithelial cells. In this study the results of SpsA binding to human, mouse, and chimeric SC strongly supported the human specificity of this unique interaction and suggested that binding sites in the third and fourth Ig-like domain of human SC (D3 and D4, respectively) are involved in SpsA-pIgR complex formation. Binding of SpsA to SC-derived synthetic peptides indicated surface-located potential binding motifs in D3 and D4. Adherence and uptake of pneumococci or SpsA-coated latex beads depended on the SpsA hexapeptide motif as well as SpsA-binding sites in D3 and D4 of human pIgR. The involvement of D3 and D4 in adherence and invasion was demonstrated by the lack of binding of SpsA-coated latex beads to transfected epithelial cells expressing mutated pIgR. Finally, blocking experiments with chimeric human-mouse SC as well as synthetic peptides indicated the participation of D3 and a key role of D4 in pneumococcal invasion.

Received for publication, September 23, 2003 , and in revised form, October 29, 2003.

^* This work was supported in part by Deutsche Forschungsgemeinschaft Sonderforschungsbereich Grant 587/479, Teilprojekt A6/A7 (to S. H.), the Bundesministerium für Bildung und Forschung (CAPNetz to S. H. and G. S. C.), and the Research Council of Norway (to R. B. and F.-E. J.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains Fig. 5S.

Both authors contributed equally to this work.

To whom correspondence should be addressed: Research Center for Infectious Diseases, University of Würzburg, Röntgenring 11, Würzburg D-97070, Germany. Tel.: 49-931-31-2153; Fax: 49-931-31-2578; E-mail: s.hammerschmidt{at}mail.uni-wuerzburg.de.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				L. E. Cron, H. J. Bootsma, N. Noske, P. Burghout, S. Hammerschmidt, and P. W. M. Hermans Surface-associated lipoprotein PpmA of Streptococcus pneumoniae is involved in colonization in a strain-specific manner Microbiology, July 1, 2009; 155(7): 2401 - 2410. [Abstract] [Full Text] [PDF]

				S. Bergmann, A. Lang, M. Rohde, V. Agarwal, C. Rennemeier, C. Grashoff, K. T. Preissner, and S. Hammerschmidt Integrin-linked kinase is required for vitronectin-mediated internalization of Streptococcus pneumoniae by host cells J. Cell Sci., January 15, 2009; 122(2): 256 - 267. [Abstract] [Full Text] [PDF]

				M. Yamaguchi, Y. Terao, Y. Mori, S. Hamada, and S. Kawabata PfbA, a Novel Plasmin- and Fibronectin-binding Protein of Streptococcus pneumoniae, Contributes to Fibronectin-dependent Adhesion and Antiphagocytosis J. Biol. Chem., December 26, 2008; 283(52): 36272 - 36279. [Abstract] [Full Text] [PDF]

				L. Lu, Z. Ma, T. S. Jokiranta, A. R. Whitney, F. R. DeLeo, and J.-R. Zhang Species-Specific Interaction of Streptococcus pneumoniae with Human Complement Factor H J. Immunol., November 15, 2008; 181(10): 7138 - 7146. [Abstract] [Full Text] [PDF]

				L. R. Quin, C. Onwubiko, Q. C. Moore, M. F. Mills, L. S. McDaniel, and S. Carmicle Factor H Binding to PspC of Streptococcus pneumoniae Increases Adherence to Human Cell Lines In Vitro and Enhances Invasion of Mouse Lungs In Vivo Infect. Immun., August 1, 2007; 75(8): 4082 - 4087. [Abstract] [Full Text] [PDF]

				A. Bonner, C. Perrier, B. Corthesy, and S. J. Perkins Solution Structure of Human Secretory Component and Implications for Biological Function J. Biol. Chem., June 8, 2007; 282(23): 16969 - 16980. [Abstract] [Full Text] [PDF]

				S. Hammerschmidt, V. Agarwal, A. Kunert, S. Haelbich, C. Skerka, and P. F. Zipfel The Host Immune Regulator Factor H Interacts via Two Contact Sites with the PspC Protein of Streptococcus pneumoniae and Mediates Adhesion to Host Epithelial Cells J. Immunol., May 1, 2007; 178(9): 5848 - 5858. [Abstract] [Full Text] [PDF]

				Z. Ma and J.-R. Zhang RR06 Activates Transcription of spr1996 and cbpA in Streptococcus pneumoniae J. Bacteriol., March 15, 2007; 189(6): 2497 - 2509. [Abstract] [Full Text] [PDF]

				R. Braathen, V. S. Hohman, P. Brandtzaeg, and F.-E. Johansen Secretory Antibody Formation: Conserved Binding Interactions between J Chain and Polymeric Ig Receptor from Humans and Amphibians J. Immunol., February 1, 2007; 178(3): 1589 - 1597. [Abstract] [Full Text] [PDF]

				R. M. A. Graham and J. C. Paton Differential Role of CbpA and PspA in Modulation of In Vitro CXC Chemokine Responses of Respiratory Epithelial Cells to Infection with Streptococcus pneumoniae Infect. Immun., December 1, 2006; 74(12): 6739 - 6749. [Abstract] [Full Text] [PDF]

				A. R. Kerr, G. K. Paterson, J. McCluskey, F. Iannelli, M. R. Oggioni, G. Pozzi, and T. J. Mitchell The Contribution of PspC to Pneumococcal Virulence Varies between Strains and Is Accomplished by Both Complement Evasion and Complement-Independent Mechanisms Infect. Immun., September 1, 2006; 74(9): 5319 - 5324. [Abstract] [Full Text] [PDF]

				L. Lu, Y. Ma, and J.-R. Zhang Streptococcus pneumoniae Recruits Complement Factor H through the Amino Terminus of CbpA J. Biol. Chem., June 2, 2006; 281(22): 15464 - 15474. [Abstract] [Full Text] [PDF]

				J. Kolberg, A. Aase, S. Bergmann, T. K. Herstad, G. Rodal, R. Frank, M. Rohde, and S. Hammerschmidt Streptococcus pneumoniae enolase is important for plasminogen binding despite low abundance of enolase protein on the bacterial cell surface. Microbiology, May 1, 2006; 152(Pt 5): 1307 - 1317. [Abstract] [Full Text] [PDF]

				B. Schmeck, S. Huber, K. Moog, J. Zahlten, A. C. Hocke, B. Opitz, S. Hammerschmidt, T. J. Mitchell, M. Kracht, S. Rosseau, et al. Pneumococci induced TLR- and Rac1-dependent NF-{kappa}B-recruitment to the IL-8 promoter in lung epithelial cells Am J Physiol Lung Cell Mol Physiol, April 1, 2006; 290(4): L730 - L737. [Abstract] [Full Text] [PDF]

				R. Braathen, A. Sandvik, G. Berntzen, S. Hammerschmidt, B. Fleckenstein, I. Sandlie, P. Brandtzaeg, F.-E. Johansen, and V. Lauvrak Identification of a Polymeric Ig Receptor Binding Phage-displayed Peptide That Exploits Epithelial Transcytosis without Dimeric IgA Competition J. Biol. Chem., March 17, 2006; 281(11): 7075 - 7081. [Abstract] [Full Text] [PDF]

				S. Bergmann and S. Hammerschmidt Versatility of pneumococcal surface proteins Microbiology, February 1, 2006; 152(2): 295 - 303. [Abstract] [Full Text] [PDF]

				P. W. M. Hermans, P. V. Adrian, C. Albert, S. Estevao, T. Hoogenboezem, I. H. T. Luijendijk, T. Kamphausen, and S. Hammerschmidt The Streptococcal Lipoprotein Rotamase A (SlrA) Is a Functional Peptidyl-prolyl Isomerase Involved in Pneumococcal Colonization J. Biol. Chem., January 13, 2006; 281(2): 968 - 976. [Abstract] [Full Text] [PDF]

				S. Hammerschmidt, S. Wolff, A. Hocke, S. Rosseau, E. Muller, and M. Rohde Illustration of Pneumococcal Polysaccharide Capsule during Adherence and Invasion of Epithelial Cells Infect. Immun., August 1, 2005; 73(8): 4653 - 4667. [Abstract] [Full Text] [PDF]

				D. Pracht, C. Elm, J. Gerber, S. Bergmann, M. Rohde, M. Seiler, K. S. Kim, H. F. Jenkinson, R. Nau, and S. Hammerschmidt PavA of Streptococcus pneumoniae Modulates Adherence, Invasion, and Meningeal Inflammation Infect. Immun., May 1, 2005; 73(5): 2680 - 2689. [Abstract] [Full Text] [PDF]

				K. Sun, F.-E. Johansen, L. Eckmann, and D. W. Metzger An Important Role for Polymeric Ig Receptor-Mediated Transport of IgA in Protection against Streptococcus pneumoniae Nasopharyngeal Carriage J. Immunol., October 1, 2004; 173(7): 4576 - 4581. [Abstract] [Full Text] [PDF]