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J. Biol. Chem., Vol. 279, Issue 8, 6674-6682, February 20, 2004

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The Amino-terminal Portion of the F-box Protein Met30p Mediates Its Nuclear Import and Assimilation into an SCF Complex^*

Lee Ellen Brunson, Cheryl Dixon, Lukasz Kozubowski, and Neal Mathias

From the Department of Biochemistry and Molecular Biology, Louisiana State University Health Science Center, Shreveport, Louisiana 71130-3932

SCF complexes are a conserved family of ubiquitin ligases composed of a common core of components and a variable component called an F-box protein that defines substrate specificity. The F-box motif links the F-box protein to the core components via its interaction with Skp1p. In yeast, the SCF^Met30p complex contains the Met30p F-box protein and regulates Met4p, a transcription factor that mediates sulfur fixation and methionine biosynthesis. Although a nuclear protein, Met30p lacks a definable nuclear localization sequence. Here we show that the entire amino-terminal half of Met30p is required for its proper nuclear localization. Mutations in the F-box, but not mutations in Skp1p, affect Met30p nuclear localization, indicating that the F-box motif plays an important role in Met30p trafficking independent of its interaction with Skp1p binding. Met30p mutants that poorly localize to the nucleus display increased nuclear to cytoplasmic exchange, indicating that the amino terminus mediates nuclear retention in addition to nuclear import. The Met30p F-box motif, residues 180–225, is necessary and sufficient to bind Skp1p; however, mutations upstream of the Met30p F-box inhibit Skp1p binding. We propose that additional factors bind the amino-terminal region of Met30p and mediate its nuclear localization and assimilation into an SCF complex.

Received for publication, August 12, 2003 , and in revised form, November 13, 2003.

^* This work was supported by National Science Foundation Grant MCB 0090403 (to N. M.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Dept. of Biochemistry and Molecular Biology, Louisiana State University Health Science Center, 1501 Kings Hwy., Shreveport, LA 71130-3932. Tel.: 318-675-8216; Fax: 318-675-5180; E-mail: pmathi{at}lsuhsc.edu.

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