HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 1, 448-457, January 7, 2005

This Article Full Text Full Text (PDF) All Versions of this Article: 280/1/448    most recent M408435200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Parent, M. Articles by Satoh, M. S. Search for Related Content PubMed PubMed Citation Articles by Parent, M. Articles by Satoh, M. S. Social Bookmarking                      What's this?

Poly(ADP-ribose) Polymerase-1 Is a Negative Regulator of HIV-1 Transcription through Competitive Binding to TAR RNA with Tat·Positive Transcription Elongation Factor b (p-TEFb) Complex^*

Marianne Parent, Tetsu M. C. Yung, Ann Rancourt, Erick L. Y. Ho, Stéphane Vispé, Fumihiko Suzuki-Matsuda¶, Aki Uehara¶, Tadashi Wada¶, Hiroshi Handa¶, and Masahiko S. Satoh, A salary support award recipient from the Canadian Institutes of Health Research and NCIC||

From the Division of Health and Environmental Research, Laval University Medical Center (CHUL), and Department of Anatomy and Physiology, Faculty of Medicine, Laval University, 2705 Blvd Laurier, Ste-Foy, Quebec G1V 4G2, Canada and the ^¶Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8503, Japan

Human immunodeficiency virus, type 1 (HIV-1) transcription is regulated by a virus-encoded protein, Tat, which forms a complex with a host cellular factor, positive transcription elongation factor b (P-TEFb). When this complex binds to TAR RNA synthesized from the HIV-1 long terminal repeat promoter element, transcription is trans-activated. In this study we showed that, in host cells, HIV-1 transcription is negatively regulated by competition of poly(ADP-ribose) polymerase-1 (PARP-1) with Tat·P-TEFb for binding to TAR RNA. PARP-1, which has a high affinity for TAR RNA (K_D = 1.35 x 10^-10 M), binds to the loop region of TAR RNA and displaces Tat or Tat·P-TEFb from the RNA. In vitro transcription assays showed that this displacement leads to suppression of Tat-mediated trans-activation of transcription. Furthermore in vivo expression of luciferase or destabilized enhanced green fluorescent protein genes under the control of the HIV-1 long terminal repeat promoter was suppressed by PARP-1. Thus, these results suggest that PARP-1 acts as a negative regulator of HIV-1 transcription through competitive binding with Tat or the Tat·P-TEFb complex to TAR RNA.

Received for publication, July 26, 2004 , and in revised form, October 13, 2004.

^* This work was supported by the National Cancer Institute of Canada (NCIC) (to M. S. S.) and a grant of Research and Development Projects in Cooperation with Academic Institutions from New Energy and Industrial Technology Development Organization (NEDO) (to H. H.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Both authors contributed equally to this work.

^|| To whom correspondence should be addressed. Tel.: 418-656-4141 (ext. 47340); Fax: 418-654-2159; E-mail: Masahiko.sato{at}crchul.ulaval.ca.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				R. Jochmann, M. Thurau, S. Jung, C. Hofmann, E. Naschberger, E. Kremmer, T. Harrer, M. Miller, N. Schaft, and M. Sturzl O-Linked N-Acetylglucosaminylation of Sp1 Inhibits the Human Immunodeficiency Virus Type 1 Promoter J. Virol., April 15, 2009; 83(8): 3704 - 3718. [Abstract] [Full Text] [PDF]

				M. Malanga, A. Czubaty, A. Girstun, K. Staron, and F. R. Althaus Poly(ADP-ribose) Binds to the Splicing Factor ASF/SF2 and Regulates Its Phosphorylation by DNA Topoisomerase I J. Biol. Chem., July 18, 2008; 283(29): 19991 - 19998. [Abstract] [Full Text] [PDF]

				Y. Chen, M. P. Schnetz, C. E. Irarrazabal, R.-F. Shen, C. K. Williams, M. B. Burg, and J. D. Ferraris Proteomic identification of proteins associated with the osmoregulatory transcription factor TonEBP/OREBP: functional effects of Hsp90 and PARP-1 Am J Physiol Renal Physiol, March 1, 2007; 292(3): F981 - F992. [Abstract] [Full Text] [PDF]