HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 10, 8637-8639, March 11, 2005

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 280/10/8637    most recent C500017200v4 C500017200v3 C500017200v2 C500017200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wang, L. Articles by Engelke, D. R. Search for Related Content PubMed PubMed Citation Articles by Wang, L. Articles by Engelke, D. R. Social Bookmarking                      What's this?

Silencing Near tRNA Genes Requires Nucleolar Localization^*

Li Wang¶, Rebecca A. Haeusler¶, Paul D. Good¶, Martin Thompson||, Sapna Nagar, and David R. Engelke**

From the Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109-0606, the Department of Chemistry, The University of Michigan, Ann Arbor, Michigan 48109-1055, and the ^||Department of Chemistry, Michigan Technological University, Houghton, Michigan 49931-129

Transcription by RNA polymerase II is antagonized by the presence of a nearby tRNA gene in Saccharomyces cerevisiae. To test hypotheses concerning the mechanism of this tRNA gene-mediated (tgm) silencing, the effects of specific gene deletions were determined. The results show that the mechanism of silencing near tRNA genes is fundamentally different from other forms of transcriptional silencing in yeast. Rather, tgm silencing is dependent on the ability to cluster the dispersed tRNA genes in or near the nucleolus, constituting a form of three-dimensional gene control.

Received for publication, January 13, 2005

^* This work was supported by National Institutes of Health Grant GM063142 (to D. R. E.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains a supplemental Table.

^¶ These authors contributed equally to this work.

^** To whom correspondence should be addressed: Dept. of Biological Chemistry, University of Michigan, 1150 W. Medical Center Dr., 3200 MSRB III, Ann Arbor, MI, 48109-0606. Tel.: 734-763-0641; Fax: 734-763-7799; E-mail: engelke{at}umich.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				T. A. Simms, S. L. Dugas, J. C. Gremillion, M. E. Ibos, M. N. Dandurand, T. T. Toliver, D. J. Edwards, and D. Donze TFIIIC Binding Sites Function as both Heterochromatin Barriers and Chromatin Insulators in Saccharomyces cerevisiae Eukaryot. Cell, December 1, 2008; 7(12): 2078 - 2086. [Abstract] [Full Text] [PDF]

				R. A. Haeusler, M. Pratt-Hyatt, P. D. Good, T. A. Gipson, and D. R. Engelke Clustering of yeast tRNA genes is mediated by specific association of condensin with tRNA gene transcription complexes Genes & Dev., August 15, 2008; 22(16): 2204 - 2214. [Abstract] [Full Text] [PDF]

				H. Liu, J. Huang, J. Wang, S. Jiang, A. S. Bailey, D. C. Goldman, M. Welcker, V. Bedell, M. L. Slovak, B. Clurman, et al. Transvection mediated by the translocated cyclin D1 locus in mantle cell lymphoma J. Exp. Med., August 4, 2008; 205(8): 1843 - 1858. [Abstract] [Full Text] [PDF]

				W. A. Decatur and M. N. Schnare Different Mechanisms for Pseudouridine Formation in Yeast 5S and 5.8S rRNAs Mol. Cell. Biol., May 15, 2008; 28(10): 3089 - 3100. [Abstract] [Full Text] [PDF]

				R. A. Haeusler and D. R. Engelke Spatial organization of transcription by RNA polymerase III Nucleic Acids Res., October 18, 2006; 34(17): 4826 - 4836. [Abstract] [Full Text] [PDF]

				R. D. Moir, J. Lee, R. A. Haeusler, N. Desai, D. R. Engelke, and I. M. Willis Protein kinase A regulates RNA polymerase III transcription through the nuclear localization of Maf1 PNAS, October 10, 2006; 103(41): 15044 - 15049. [Abstract] [Full Text] [PDF]

				H. Nishihara, A. F.A. Smit, and N. Okada Functional noncoding sequences derived from SINEs in the mammalian genome Genome Res., July 1, 2006; 16(7): 864 - 874. [Abstract] [Full Text] [PDF]

				M.-K. Kim, K. C. Claiborn, and H. L. Levin The Long Terminal Repeat-Containing Retrotransposon Tf1 Possesses Amino Acids in Gag That Regulate Nuclear Localization and Particle Formation J. Virol., August 1, 2005; 79(15): 9540 - 9555. [Abstract] [Full Text] [PDF]