|
Originally published In Press as doi:10.1074/jbc.M413867200 on January 4, 2005
Originally published In Press as doi:10.1074/jbc.M413867200 on December 28, 2004
J. Biol. Chem., Vol. 280, Issue 10, 9489-9497, March 11, 2005
PIG-V Involved in Transferring the Second Mannose in Glycosylphosphatidylinositol*
Ji Young Kang ,
Yeongjin Hong ||,
Hisashi Ashida ,
Nobue Shishioh ,
Yoshiko Murakami ,
Yasu S. Morita ,
Yusuke Maeda , and
Taroh Kinoshita ¶
From the
Department of Immunoregulation, Research Institute for Microbial Diseases, 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan and the Department of Microbiology, Genomic Research Center for Enteropathogenic Bacteria, Chonnam National University Medical School, Gwangju 501-746, South Korea
Glycosylphosphatidylinositol (GPI) is a glycolipid that anchors many proteins to the eukaryotic cell surface. The biosynthetic pathway of GPI is mediated by sequential additions of sugars and other components to phosphatidylinositol. Four mannoses in the GPI are transferred from dolichol-phosphate-mannose (Dol-P-Man) and are linked through different glycosidic linkages. Therefore, four Dol-P-Man-dependent mannosyltransferases, GPI-MT-I, -MT-II, -MT-III, and -MT-IV for the first, second, third, and fourth mannoses, respectively, are required for generation of GPI. GPI-MT-I (PIG-M), GPI-MT-III (PIG-B), and GPI-MT-IV (SMP3) were previously reported, but GPI-MT-II remains to be identified. Here we report the cloning of PIG-V involved in transferring the second mannose in the GPI anchor. Human PIG-V encodes a 493-amino acid, endoplasmic reticulum (ER) resident protein with eight putative transmembrane regions. Saccharomyces cerevisiae protein encoded in open reading frame YBR004c, which we termed GPI18, has 25% amino acid identity to human PIG-V. Viability of the yeast gpi18 deletion mutant was restored by human PIG-V cDNA. PIG-V has two functionally important conserved regions facing the ER lumen. Taken together, we suggest that PIG-V is the second mannosyltransferase in GPI anchor biosynthesis.
Received for publication, December 9, 2004
, and in revised form, December 27, 2004.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AB196341.
* This work was supported in part by grants from the Ministry of Education, Science, Sports, Culture and Technology of Japan. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
|| Supported in part by a grant from the Ministry of Health and Welfare of the Republic of Korea (01-PJ10-PG6-01GM00-0002).
¶ To whom correspondence should be addressed. Tel.: 81-6-6879-8328; Fax: 81-6-6875-5233; E-mail tkinoshi{at}biken.osaka-u.ac.jp.

CiteULike Complore Connotea Del.icio.us Digg Reddit Technorati What's this?
This article has been cited by other articles:

|
 |

|
 |
 
D. Kaur, M. R. McNeil, K.-H. Khoo, D. Chatterjee, D. C. Crick, M. Jackson, and P. J. Brennan
New Insights into the Biosynthesis of Mycobacterial Lipomannan Arising from Deletion of a Conserved Gene
J. Biol. Chem.,
September 14, 2007;
282(37):
27133 - 27140.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
K. Sato, Y. Noda, and K. Yoda
Pga1 Is an Essential Component of Glycosylphosphatidylinositol-Mannosyltransferase II of Saccharomyces cerevisiae
Mol. Biol. Cell,
September 1, 2007;
18(9):
3472 - 3485.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y. U. Kim, H. Ashida, K. Mori, Y. Maeda, Y. Hong, and T. Kinoshita
Both Mammalian PIG-M and PIG-X are Required for Growth of GPI14-Disrupted Yeast
J. Biochem.,
July 1, 2007;
142(1):
123 - 129.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Berg, D. Kaur, M. Jackson, and P. J Brennan
The glycosyltransferases of Mycobacterium tuberculosis--roles in the synthesis of arabinogalactan, lipoarabinomannan, and other glycoconjugates
Glycobiology,
June 1, 2007;
17(6):
35R - 56R.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
P. Orlean and A. K. Menon
Thematic review series: Lipid Posttranslational Modifications. GPI anchoring of protein in yeast and mammalian cells, or: how we learned to stop worrying and love glycophospholipids
J. Lipid Res.,
May 1, 2007;
48(5):
993 - 1011.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y. S. Morita, C. B. C. Sena, R. F. Waller, K. Kurokawa, M. F. Sernee, F. Nakatani, R. E. Haites, H. Billman-Jacobe, M. J. McConville, Y. Maeda, et al.
PimE Is a Polyprenol-phosphate-mannose-dependent Mannosyltransferase That Transfers the Fifth Mannose of Phosphatidylinositol Mannoside in Mycobacteria
J. Biol. Chem.,
September 1, 2006;
281(35):
25143 - 25155.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
H. Ashida, Y. Maeda, and T. Kinoshita
DPM1, the Catalytic Subunit of Dolichol-phosphate Mannose Synthase, Is Tethered to and Stabilized on the Endoplasmic Reticulum Membrane by DPM3
J. Biol. Chem.,
January 13, 2006;
281(2):
896 - 904.
[Abstract]
[Full Text]
[PDF]
|
 |
|
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.
|
Advertisement
Advertisement
|