HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 10, 9646-9652, March 11, 2005

This Article Full Text Full Text (PDF) All Versions of this Article: 280/10/9646    most recent M410274200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Uemura, T. Articles by Igarashi, K. Search for Related Content PubMed PubMed Citation Articles by Uemura, T. Articles by Igarashi, K. Social Bookmarking                      What's this?

Characteristics of the Polyamine Transporter TPO1 and Regulation of Its Activity and Cellular Localization by Phosphorylation^*

Takeshi Uemura, Ken Tachihara, Hideyuki Tomitori, Keiko Kashiwagi, and Kazuei Igarashi

From the Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8675, Japan

The subcellular localization of the polyamine transporter TPO1 of Saccharomyces cerevisiae was determined by sucrose gradient centrifugation and indirect immunofluorescence microscopy. When expressed from a multi-copy vector, TPO1 was located mainly on the plasma membrane, but with some localization on the vacuolar membrane. Polyamine transport by TPO1 was dependent on pH. Uptake of spermidine and spermine occurred at alkaline pH (pH 8.0), whereas inhibition of spermidine uptake, but not spermine uptake, was observed at acidic pH (pH 5.0). This suggests that TPO1 catalyzes polyamine excretion at acidic pH, similar to the PotE transporter in Escherichia coli. Paraquat, a polyamine analogue, was excreted by TPO1 at a rate comparable with the excretion of spermidine (deduced from the inhibition of spermidine uptake) at pH 5.0. However, excretion of preloaded radiolabeled spermidine and spermine was not observed in intact cells, suggesting that preloaded spermidine (or spermine) exists mainly as spermidine (or spermine)-ribosome complex in cells. The transport activity of TPO1 was enhanced through phosphorylation at Ser¹⁹ by protein kinase C and at Thr⁵² by casein kinase 1. Sorting of TPO1 from the endoplasmic reticulum to the plasma membrane was enhanced through phosphorylation at Ser³⁴² by cAMP-dependent protein kinases 1 and 2.

Received for publication, September 7, 2004 , and in revised form, December 20, 2004.

^* This work was supported by a grant-in-aid for scientific research from the Ministry of Education, Culture, Sports, Science, and Technology, Japan. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed. Tel.: 81-43-226-2871; Fax. 81-43-226-2873; E-mail: iga16077{at}p.chiba-u.ac.jp.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				T. Uemura, H. F. Yerushalmi, G. Tsaprailis, D. E. Stringer, K. E. Pastorian, L. Hawel III, C. V. Byus, and E. W. Gerner Identification and Characterization of a Diamine Exporter in Colon Epithelial Cells J. Biol. Chem., September 26, 2008; 283(39): 26428 - 26435. [Abstract] [Full Text] [PDF]

				K. Higashi, H. Ishigure, R. Demizu, T. Uemura, K. Nishino, A. Yamaguchi, K. Kashiwagi, and K. Igarashi Identification of a Spermidine Excretion Protein Complex (MdtJI) in Escherichia coli J. Bacteriol., February 1, 2008; 190(3): 872 - 878. [Abstract] [Full Text] [PDF]

				T. Uemura, K. Kashiwagi, and K. Igarashi Polyamine Uptake by DUR3 and SAM3 in Saccharomyces cerevisiae J. Biol. Chem., March 9, 2007; 282(10): 7733 - 7741. [Abstract] [Full Text] [PDF]

				A. K. Mattoo, A. P. Sobolev, A. Neelam, R. K. Goyal, A. K. Handa, and A. L. Segre Nuclear Magnetic Resonance Spectroscopy-Based Metabolite Profiling of Transgenic Tomato Fruit Engineered to Accumulate Spermidine and Spermine Reveals Enhanced Anabolic and Nitrogen-Carbon Interactions Plant Physiology, December 1, 2006; 142(4): 1759 - 1770. [Abstract] [Full Text] [PDF]

				N. N. Tun, C. Santa-Catarina, T. Begum, V. Silveira, W. Handro, E. I. S. Floh, and G. F. E. Scherer Polyamines Induce Rapid Biosynthesis of Nitric Oxide (NO) in Arabidopsis thaliana Seedlings Plant Cell Physiol., March 1, 2006; 47(3): 346 - 354. [Abstract] [Full Text] [PDF]

				K. Hoshino, E. Momiyama, K. Yoshida, K. Nishimura, S. Sakai, T. Toida, K. Kashiwagi, and K. Igarashi Polyamine Transport by Mammalian Cells and Mitochondria: ROLE OF ANTIZYME AND GLYCOSAMINOGLYCANS J. Biol. Chem., December 30, 2005; 280(52): 42801 - 42808. [Abstract] [Full Text] [PDF]

				M. Aouida, A. Leduc, R. Poulin, and D. Ramotar AGP2 Encodes the Major Permease for High Affinity Polyamine Import in Saccharomyces cerevisiae J. Biol. Chem., June 24, 2005; 280(25): 24267 - 24276. [Abstract] [Full Text] [PDF]