HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 12, 11981-11986, March 25, 2005

This Article Full Text Full Text (PDF) All Versions of this Article: 280/12/11981    most recent M413971200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kilpatrick, Z. E. Articles by Kumar, S. Search for Related Content PubMed PubMed Citation Articles by Kilpatrick, Z. E. Articles by Kumar, S. Social Bookmarking                      What's this?

Ecdysone-mediated Up-regulation of the Effector Caspase DRICE Is Required for Hormone-dependent Apoptosis in Drosophila Cells^*

Zoé E. Kilpatrick, Dimitrios Cakouros, and Sharad Kumar

From the Hanson Institute, Institute of Medical and Veterinary Science, PO Box 14, Rundle Mall, Adelaide, SA 5000, Australia

The Drosophila steroid hormone ecdysone mediates cell death during metamorphosis by regulating the transcription of a number of cell death genes. The apical caspase DRONC is known to be transcriptionally regulated by ecdysone during development. Here we demonstrate that ecdysone also regulates the transcription of DRICE, a major effector caspase and a downstream target for DRONC in the fly. Using RNA interference in an ecdysone-responsive Drosophila cell line, we show that drice up-regulation is essential for apoptosis induced by ecdysone. We also show that drice expression is specifically controlled by the ecdysone-regulated transcription factor BR-C. Combined with previous observations, our results indicate that transcriptional regulation of the components of the core apoptotic machinery plays a key role in hormone-regulated programmed cell death during Drosophila development.

Received for publication, December 13, 2004 , and in revised form, January 10, 2005.

^* This work was supported by the National Health and Medical Research Council of Australia. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Supported by an Australian Postgraduate Award.

To whom correspondence should be addressed. Fax: 61-8-8222-3139; E-mail: sharad.kumar{at}imvs.sa.gov.au.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				Y. Liu, Z. Sheng, H. Liu, D. Wen, Q. He, S. Wang, W. Shao, R.-J. Jiang, S. An, Y. Sun, et al. Juvenile hormone counteracts the bHLH-PAS transcription factors MET and GCE to prevent caspase-dependent programmed cell death in Drosophila Development, June 15, 2009; 136(12): 2015 - 2025. [Abstract] [Full Text] [PDF]

				D. A. Primrose, S. Chaudhry, A. G. D. Johnson, A. Hrdlicka, A. Schindler, D. Tran, and E. Foley Interactions of DNR1 with the apoptotic machinery of Drosophila melanogaster J. Cell Sci., April 1, 2007; 120(7): 1189 - 1199. [Abstract] [Full Text] [PDF]

				C. W. Maurer, M. Chiorazzi, and S. Shaham Timing of the onset of a developmental cell death is controlled by transcriptional induction of the C. elegans ced-3 caspase-encoding gene Development, April 1, 2007; 134(7): 1357 - 1368. [Abstract] [Full Text] [PDF]

				B. Santelices and J. Alvarado Applying the concept of metamorphosis to the crustose-to-erect thallus transition of macroalgae Integr. Comp. Biol., December 1, 2006; 46(6): 713 - 718. [Abstract] [Full Text] [PDF]