HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 14, 13600-13605, April 8, 2005

This Article Full Text Full Text (PDF) All Versions of this Article: 280/14/13600    most recent M409468200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yu, C. Articles by Cohen, R. N. Search for Related Content PubMed PubMed Citation Articles by Yu, C. Articles by Cohen, R. N. Social Bookmarking                      What's this?

The Nuclear Receptor Corepressors NCoR and SMRT Decrease Peroxisome Proliferator-activated Receptor Transcriptional Activity and Repress 3T3-L1 Adipogenesis^*

Christine Yu¶, Kathleen Markan, Karla A. Temple, Dianne Deplewski, Matthew J. Brady, and Ronald N. Cohen||

From the Section of Endocrinology, Department of Medicine and the Section of Pediatric Endocrinology, Department of Pediatrics, The University of Chicago, Chicago, Illinois 60637

The peroxisome proliferator-activated receptor (PPAR) is a central regulator of adipogenesis and recruits coactivator proteins in response to ligand. However, the role of another class of nuclear cofactors, the nuclear receptor corepressors, in modulating PPAR transcriptional activity is less clear. Such corepressors include the nuclear receptor corepressor (NCoR) and the silencing mediator of retinoid and thyroid hormone receptors (SMRT). Our data suggest that PPAR recruits SMRT and NCoR in the absence of ligand and that these corepressors are capable of down-regulating PPAR-mediated transcriptional activity. The addition of the PPAR ligand pioglitazone results in dissociation of the PPAR-corepressor complex. To define the role of SMRT and NCoR in PPAR action, 3T3-L1 cells deficient in SMRT or NCoR were generated by RNA interference. When these cells are exposed to differentiation media, they exhibit increased expression of adipocyte-specific genes and increased production of lipid droplets, as compared with control cells. These data suggest that the nuclear receptor corepressors decrease PPAR transcriptional activity and repress the adipogenic program in 3T3-L1 cells.

Received for publication, August 18, 2004 , and in revised form, January 21, 2005.

^* This work was supported in part by Pilot and Feasibility Study P60 DK20595 through the University of Chicago Diabetes Research and Training Center. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ Recipient of a grant from the Endocrine Fellows Foundation.

^|| To whom correspondence should be addressed: 5841 S. Maryland Ave., MC 1027, Chicago, IL 60637. Tel.: 773-702-9653; Fax: 773-834-0486; E-mail: roncohen{at}medicine.bsd.uchicago.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				F. Ondrey Peroxisome Proliferator-Activated Receptor {gamma} Pathway Targeting in Carcinogenesis: Implications for Chemoprevention Clin. Cancer Res., January 1, 2009; 15(1): 2 - 8. [Abstract] [Full Text] [PDF]

				J. T. M. Tan, S. V. McLennan, W. W. Song, L. W.-Y. Lo, J. G. Bonner, P. F. Williams, and S. M. Twigg Connective tissue growth factor inhibits adipocyte differentiation Am J Physiol Cell Physiol, September 1, 2008; 295(3): C740 - C751. [Abstract] [Full Text] [PDF]

				M. H. Liu, J. Li, P. Shen, B. Husna, E. S. Tai, and E. L. Yong A Natural Polymorphism in Peroxisome Proliferator-Activated Receptor-{alpha} Hinge Region Attenuates Transcription due to Defective Release of Nuclear Receptor Corepressor from Chromatin Mol. Endocrinol., May 1, 2008; 22(5): 1078 - 1092. [Abstract] [Full Text] [PDF]

				Y. H. Yu, S. C. Wu, W. T. K. Cheng, H. J. Mersmann, and S. T. Ding Ectopic expression of porcine peroxisome proliferator-activated receptor {delta} regulates adipogenesis in mouse myoblasts J Anim Sci, January 1, 2008; 86(1): 64 - 72. [Abstract] [Full Text] [PDF]

				S. Kume, T. Uzu, S.-i. Araki, T. Sugimoto, K. Isshiki, M. Chin-Kanasaki, M. Sakaguchi, N. Kubota, Y. Terauchi, T. Kadowaki, et al. Role of Altered Renal Lipid Metabolism in the Development of Renal Injury Induced by a High-Fat Diet J. Am. Soc. Nephrol., October 1, 2007; 18(10): 2715 - 2723. [Abstract] [Full Text] [PDF]

				J. N. Feige, L. Gelman, D. Rossi, V. Zoete, R. Metivier, C. Tudor, S. I. Anghel, A. Grosdidier, C. Lathion, Y. Engelborghs, et al. The Endocrine Disruptor Monoethyl-hexyl-phthalate Is a Selective Peroxisome Proliferator-activated Receptor {gamma} Modulator That Promotes Adipogenesis J. Biol. Chem., June 29, 2007; 282(26): 19152 - 19166. [Abstract] [Full Text] [PDF]

				E. H. Jeninga, O. van Beekum, A. D. J. van Dijk, N. Hamers, B. I. Hendriks-Stegeman, A. M. J. J. Bonvin, R. Berger, and E. Kalkhoven Impaired Peroxisome Proliferator-Activated Receptor {gamma} Function through Mutation of a Conserved Salt Bridge (R425C) in Familial Partial Lipodystrophy Mol. Endocrinol., May 1, 2007; 21(5): 1049 - 1065. [Abstract] [Full Text] [PDF]

				B. Gershman, O. Puig, L. Hang, R. M. Peitzsch, M. Tatar, and R. S. Garofalo High-resolution dynamics of the transcriptional response to nutrition in Drosophila: a key role for dFOXO Physiol Genomics, March 14, 2007; 29(1): 24 - 34. [Abstract] [Full Text] [PDF]

				L. Michalik, J. Auwerx, J. P. Berger, V. K. Chatterjee, C. K. Glass, F. J. Gonzalez, P. A. Grimaldi, T. Kadowaki, M. A. Lazar, S. O'Rahilly, et al. International Union of Pharmacology. LXI. Peroxisome Proliferator-Activated Receptors Pharmacol. Rev., December 1, 2006; 58(4): 726 - 741. [Abstract] [Full Text] [PDF]

				D. Nichol, M. Christian, J. H. Steel, R. White, and M. G. Parker RIP140 Expression Is Stimulated by Estrogen-related Receptor {alpha} during Adipogenesis J. Biol. Chem., October 27, 2006; 281(43): 32140 - 32147. [Abstract] [Full Text] [PDF]

				R. A. Winn, M. Van Scoyk, M. Hammond, K. Rodriguez, J. T. Crossno Jr., L. E. Heasley, and R. A. Nemenoff Antitumorigenic Effect of Wnt 7a and Fzd 9 in Non-small Cell Lung Cancer Cells Is Mediated through ERK-5-dependent Activation of Peroxisome Proliferator-activated Receptor {gamma} J. Biol. Chem., September 15, 2006; 281(37): 26943 - 26950. [Abstract] [Full Text] [PDF]

				M. Schupp, M. Clemenz, R. Gineste, H. Witt, J. Janke, S. Helleboid, N. Hennuyer, P. Ruiz, T. Unger, B. Staels, et al. Molecular Characterization of New Selective Peroxisome Proliferator-Activated Receptor {gamma} Modulators With Angiotensin Receptor Blocking Activity Diabetes, December 1, 2005; 54(12): 3442 - 3452. [Abstract] [Full Text] [PDF]