JBC Origene Your Gene Company

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


Originally published In Press as doi:10.1074/jbc.M500329200 on February 21, 2005

J. Biol. Chem., Vol. 280, Issue 17, 16586-16593, April 29, 2005
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
280/17/16586    most recent
M500329200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Voisin, S.
Right arrow Articles by Logan, S. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Voisin, S.
Right arrow Articles by Logan, S. M.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Identification and Characterization of the Unique N-Linked Glycan Common to the Flagellins and S-layer Glycoprotein of Methanococcus voltae*{boxs}

Sebastien Voisin{ddagger}, R. Scott Houliston{ddagger}, John Kelly{ddagger}, Jean-Robert Brisson{ddagger}, David Watson{ddagger}, Sonia L. Bardy§, Ken F. Jarrell§, and Susan M. Logan{ddagger}||

From the {ddagger}Institute for Biological Sciences, National Research Council, Ottawa, Ontario K1A OR6 and the §Department of Microbiology and Immunology, Queens University, Kingston, Ontario K7L 3N6, Canada

The flagellum of Methanococcus voltae is composed of four structural flagellin proteins FlaA, FlaB1, FlaB2, and FlaB3. These proteins possess a total of 15 potential N-linked sequons (NX(S/T)) and show a mass shift on an SDS-polyacrylamide gel indicating significant post-translational modification. We describe here the structural characterization of the flagellin glycan from M. voltae using mass spectrometry to examine the proteolytic digests of the flagellin proteins in combination with NMR analysis of the purified glycan using a sensitive, cryogenically cooled probe. Nano-liquid chromatography-tandem mass spectrometry analysis of the proteolytic digests of the flagellin proteins revealed that they are post-translationally modified with a novel N-linked trisaccharide of mass 779 Da that is composed of three sugar residues with masses of 318, 258, and 203 Da, respectively. In every instance the glycan is attached to the peptide through the asparagine residue of a typical N-linked sequon. The glycan modification has been observed on 14 of the 15 sequon sites present on the four flagellin structural proteins. The novel glycan structure elucidated by NMR analysis was shown to be a trisaccharide composed of {beta}-ManpNAcA6Thr-(1–4)-{beta}-Glc-pNAc3NAcA-(1–3)-{beta}-GlcpNAc linked to Asn. In addition, the same trisaccharide was identified on a tryptic peptide of the S-layer protein from this organism implicating a common N-linked glycosylation pathway.


Received for publication, January 10, 2005 , and in revised form, February 10, 2005.

* This work was supported by genomic and health initiative funding (to S. M. L., S. V., and J. K.) and a discovery grant from the Natural Sciences and Engineering Council of Canada (NSERC) (to K. F. J.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

{boxs} The on-line version of this article (available at http://www.jbc.org) contains Supplemental Table 1.

Recipient of a Post-graduate Scholarship B from NSERC.

|| To whom correspondence should be addressed: Inst. of Biological Sciences, National Research Council, 100 Sussex Dr., Ottawa, Ontario K1A OR6, Canada. Tel.: 613-990-0839; Fax: 613-952-9092; E-mail: susan.logan{at}nrc-cnrc.gc.ca.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
J. Bacteriol.Home page
S. Y. M. Ng, B. Zolghadr, A. J. M. Driessen, S.-V. Albers, and K. F. Jarrell
Cell Surface Structures of Archaea
J. Bacteriol., September 15, 2008; 190(18): 6039 - 6047.
[Full Text] [PDF]


Home page
J. Bacteriol.Home page
D. J. VanDyke, J. Wu, S. Y. M. Ng, M. Kanbe, B. Chaban, S.-I. Aizawa, and K. F. Jarrell
Identification of a Putative Acetyltransferase Gene, MMP0350, Which Affects Proper Assembly of both Flagella and Pili in the Archaeon Methanococcus maripaludis
J. Bacteriol., August 1, 2008; 190(15): 5300 - 5307.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
M. Abu-Qarn, A. Giordano, F. Battaglia, A. Trauner, P. G. Hitchen, H. R. Morris, A. Dell, and J. Eichler
Identification of AglE, a Second Glycosyltransferase Involved in N Glycosylation of the Haloferax volcanii S-Layer Glycoprotein
J. Bacteriol., May 1, 2008; 190(9): 3140 - 3146.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
H. Shams-Eldin, B. Chaban, S. Niehus, R. T. Schwarz, and K. F. Jarrell
Identification of the Archaeal alg7 Gene Homolog (Encoding N-Acetylglucosamine-1-Phosphate Transferase) of the N-Linked Glycosylation System by Cross-Domain Complementation in Saccharomyces cerevisiae
J. Bacteriol., March 15, 2008; 190(6): 2217 - 2220.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
S. Voisin, J. V. Kus, S. Houliston, F. St-Michael, D. Watson, D. G. Cvitkovitch, J. Kelly, J.-R. Brisson, and L. L. Burrows
Glycosylation of Pseudomonas aeruginosa Strain Pa5196 Type IV Pilins with Mycobacterium-Like {alpha}-1,5-Linked D-Araf Oligosaccharides
J. Bacteriol., January 1, 2007; 189(1): 151 - 159.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
D. J. Nather, R. Rachel, G. Wanner, and R. Wirth
Flagella of Pyrococcus furiosus: Multifunctional Organelles, Made for Swimming, Adhesion to Various Surfaces, and Cell-Cell Contacts.
J. Bacteriol., October 1, 2006; 188(19): 6915 - 6923.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
A. Verma, M. Schirm, S. K. Arora, P. Thibault, S. M. Logan, and R. Ramphal
Glycosylation of b-Type Flagellin of Pseudomonas aeruginosa: Structural and Genetic Basis.
J. Bacteriol., June 1, 2006; 188(12): 4395 - 4403.
[Abstract] [Full Text] [PDF]


Home page
MicrobiologyHome page
P. G. Hitchen and A. Dell
Bacterial glycoproteomics
Microbiology, June 1, 2006; 152(6): 1575 - 1580.
[Abstract] [Full Text] [PDF]


Home page
MicrobiologyHome page
S. M. Logan
Flagellar glycosylation - a new component of the motility repertoire?
Microbiology, May 1, 2006; 152(Pt 5): 1249 - 1262.
[Abstract] [Full Text] [PDF]


Home page
GlycobiologyHome page
D. J. Kelleher and R. Gilmore
An evolving view of the eukaryotic oligosaccharyltransferase
Glycobiology, April 1, 2006; 16(4): 47R - 62R.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
L. L. Grochowski, H. Xu, and R. H. White
Ribose-5-Phosphate Biosynthesis in Methanocaldococcus jannaschii Occurs in the Absence of a Pentose-Phosphate Pathway
J. Bacteriol., November 1, 2005; 187(21): 7382 - 7389.
[Abstract] [Full Text] [PDF]


Home page
Microbiol. Mol. Biol. Rev.Home page
J. Eichler and M. W. W. Adams
Posttranslational Protein Modification in Archaea
Microbiol. Mol. Biol. Rev., September 1, 2005; 69(3): 393 - 425.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.