HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 17, 16851-16860, April 29, 2005

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 280/17/16851    most recent M414587200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gaildrat, P. Articles by Klein, D. C. Search for Related Content PubMed PubMed Citation Articles by Gaildrat, P. Articles by Klein, D. C. Social Bookmarking                      What's this?

A Novel Pineal-specific Product of the Oligopeptide Transporter PepT1 Gene

CIRCADIAN EXPRESSION MEDIATED BY cAMP ACTIVATION OF AN INTRONIC PROMOTER^*

Pascaline Gaildrat, Morten Møller, Sujira Mukda¶||, Ann Humphries**, David A. Carter**, Vadivel Ganapathy, and David C. Klein¶¶

From the Section on Neuroendocrinology, Laboratory of Developmental Neurobiology, NICHD, National Institutes of Health, Bethesda, Maryland 20892-4480, the Institute of Medical Anatomy, Panum Institute, University of Copenhagen, Copenhagen DK-2200, Denmark, the ^¶Neuro-Behavioural Biology Center, Institute of Science and Technology for Research and Development, Mahidol University, Salaya Campus, Nakornpathom 73170, Thailand, the ^**School of Biosciences, Cardiff University, Cardiff CF103US, United Kingdom, and the Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, Georgia 30912

The oligopeptide transporter 1, PepT1, is a member of the Slc15 family of 12 membrane-spanning domain transporters; PepT1 has proton/peptide cotransport activity and is selectively expressed in intestinal epithelial cells, where it is responsible for the nutritional absorption of di- and tri-peptides. Here, a novel PepT1 gene product has been identified in the rat pineal gland, termed pgPepT1. It encodes a 150-amino acid protein encompassing the C-terminal 3 membrane-spanning domains of intestinal PepT1 protein, with 3 additional N-terminal residues. Expression of pgPepT1 appears to be restricted to the pineal gland and follows a marked circadian pattern with >100-fold higher levels of mRNA occurring at night; this is accompanied by an accumulation of membrane-associated pgPepT1 protein (16 kDa). The daily rhythm in pgPepT1 mRNA is regulated by the well described neural pathway that controls pineal melatonin production. This includes the retina, the circadian clock in the suprachiasmatic nucleus, central structures, and projections from the superior cervical ganglia; activation of this pathway results in the release of norepinephrine. Here it was found that pgPepT1 expression is mediated by a norepinephrine cyclic AMP mechanism that activates an alternative promoter located in intron 20 of the gene. pgPepT1 protein was found to have transporter-modulator activity; it could contribute to circadian changes in pineal function through this mechanism.

Received for publication, December 27, 2004

^* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http//www.jbc.org) contains Supplemental Fig. S1.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank^TM/EBI Data Bank with accession number(s) AY860424.

^|| Supported by a Royal Golden Jubilee Ph.D. Grant from the Thailand Research Fund and Danish Medical Research Council (Grant J nr. 22-02-0288).

Supported by the Wellcome Trust.

^¶¶ To whom correspondence should be addressed: National Institutes of Health, Bldg. 49, Rm. 6A-82, Bethesda, MD 20892-4480. Tel.: 301-496-6914; Fax: 301-480-3526; E-mail: klein{at}helix.nih.gov.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				D. M. Price, R. Kanyo, N. Steinberg, C. L. Chik, and A. K. Ho Nocturnal Activation of Aurora C in Rat Pineal Gland: Its Role in the Norepinephrine-Induced Phosphorylation of Histone H3 and Gene Expression Endocrinology, May 1, 2009; 150(5): 2334 - 2341. [Abstract] [Full Text] [PDF]

				M. J. Bailey, S. L. Coon, D. A. Carter, A. Humphries, J.-s. Kim, Q. Shi, P. Gaildrat, F. Morin, S. Ganguly, J. B. Hogenesch, et al. Night/Day Changes in Pineal Expression of >600 Genes: CENTRAL ROLE OF ADRENERGIC/cAMP SIGNALING J. Biol. Chem., March 20, 2009; 284(12): 7606 - 7622. [Abstract] [Full Text] [PDF]

				M. F. Rath, M. J. Bailey, J.-S. Kim, A. K. Ho, P. Gaildrat, S. L. Coon, M. Moller, and D. C. Klein Developmental and Diurnal Dynamics of Pax4 Expression in the Mammalian Pineal Gland: Nocturnal Down-Regulation Is Mediated by Adrenergic-Cyclic Adenosine 3',5'-Monophosphate Signaling Endocrinology, February 1, 2009; 150(2): 803 - 811. [Abstract] [Full Text] [PDF]

				S. Ganguly, C. Grodzki, D. Sugden, M. Moller, S. Odom, P. Gaildrat, I. Gery, R. P. Siraganian, J. Rivera, and D. C. Klein Neural Adrenergic/Cyclic AMP Regulation of the Immunoglobulin E Receptor {alpha}-Subunit Expression in the Mammalian Pinealocyte: A NEUROENDOCRINE/IMMUNE RESPONSE LINK? J. Biol. Chem., November 9, 2007; 282(45): 32758 - 32764. [Abstract] [Full Text] [PDF]

				A. K. Ho, D. M. Price, W. G. Dukewich, N. Steinberg, T. G. Arnason, and C. L. Chik Acetylation of Histone H3 and Adrenergic-Regulated Gene Transcription in Rat Pinealocytes Endocrinology, October 1, 2007; 148(10): 4592 - 4600. [Abstract] [Full Text] [PDF]

				D. C. Klein The Pineal Gene Expression Party: Who's the Surprise Guest? Endocrinology, April 1, 2007; 148(4): 1463 - 1464. [Full Text] [PDF]

				C. L. Chik, T. G. Arnason, W. G. Dukewich, D. M. Price, A. Ranger, and A. K. Ho Histone H3 Phosphorylation in the Rat Pineal Gland: Adrenergic Regulation and Diurnal Variation Endocrinology, April 1, 2007; 148(4): 1465 - 1472. [Abstract] [Full Text] [PDF]

				J.-S. Kim, M. J. Bailey, A. K. Ho, M. Moller, P. Gaildrat, and D. C. Klein Daily Rhythm in Pineal Phosphodiesterase (PDE) Activity Reflects Adrenergic/3',5'-Cyclic Adenosine 5'-Monophosphate Induction of the PDE4B2 Variant Endocrinology, April 1, 2007; 148(4): 1475 - 1485. [Abstract] [Full Text] [PDF]