HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 17, 16994-17004, April 29, 2005

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 280/17/16994    most recent M412774200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Uchiyama, S. Articles by Fukui, K. Search for Related Content PubMed PubMed Citation Articles by Uchiyama, S. Articles by Fukui, K. Social Bookmarking                      What's this?

Proteome Analysis of Human Metaphase Chromosomes^*

Susumu Uchiyama, Shouhei Kobayashi, Hideaki Takata, Takeshi Ishihara, Naoto Hori, Tsunehito Higashi, Kayoko Hayashihara, Takefumi Sone¶, Daisuke Higo||, Takashi Nirasawa||, Toshifumi Takao**, Sachihiro Matsunaga, and Kiichi Fukui

From the Department of Biotechnology, Graduate School of Engineering, and the ^**Institute for Protein Research, Osaka University, Suita 565-0871, Japan and ^|| Bruker Daltonics K. K., Yokohama 221-0022, Japan

DNA is packaged as chromatin in the interphase nucleus. During mitosis, chromatin fibers are highly condensed to form metaphase chromosomes, which ensure equal segregation of replicated chromosomal DNA into the daughter cells. Despite >1 century of research on metaphase chromosomes, information regarding the higher order structure of metaphase chromosomes is limited, and it is still not clear which proteins are involved in further folding of the chromatin fiber into metaphase chromosomes. To obtain a global view of the chromosomal proteins, we performed proteome analyses on three types of isolated human metaphase chromosomes. We first show the results from comparative proteome analyses of two types of isolated human metaphase chromosomes that have been frequently used in biochemical and morphological analyses. 209 proteins were quantitatively identified and classified into six groups on the basis of their known interphase localization. Furthermore, a list of 107 proteins was obtained from the proteome analyses of highly purified metaphase chromosomes, the majority of which are essential for chromosome structure and function. Based on the information obtained on these proteins and on their localizations during mitosis as assessed by immunostaining, we present a four-layer model of metaphase chromosomes. According to this model, the chromosomal proteins have been newly classified into each of four groups: chromosome coating proteins, chromosome peripheral proteins, chromosome structural proteins, and chromosome fibrous proteins. This analysis represents the first compositional view of human metaphase chromosomes and provides a protein framework for future research on this topic.

Received for publication, November 11, 2004 , and in revised form, January 21, 2005.

^* This work was supported in part by special coordination funds from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (to K. F.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains Supplemental Figs. S1–S9 and Supplemental Tables S1–S3.

Both authors contributed equally to this work.

^¶ Present address: Inst. for Microbiological Diseases, Osaka University, Suita 565-0871, Japan.

To whom correspondence should be addressed. Tel.: 81-6-6879-7440; Fax: 81-6-6879-7441; E-mail: kfukui{at}bio.eng.osaka-u.ac.jp.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Nakagawa, N. Ohmido, K. Ishikawa, S. Uchiyama, K. Fukui, and T. Azuma Anti-Peptide Antibodies for Examining the Conformation, Molecular Assembly and Localization of an Intracellular Protein, Ribosomal Protein S6, In vivo J. Biochem., March 1, 2008; 143(3): 325 - 332. [Abstract] [Full Text] [PDF]

				I. M. Porter, S. E. McClelland, G. A. Khoudoli, C. J. Hunter, J. S. Andersen, A. D. McAinsh, J. J. Blow, and J. R. Swedlow Bod1, a novel kinetochore protein required for chromosome biorientation J. Cell Biol., October 22, 2007; 179(2): 187 - 197. [Abstract] [Full Text] [PDF]

				N. Ma, S. Matsunaga, H. Takata, R. Ono-Maniwa, S. Uchiyama, and K. Fukui Nucleolin functions in nucleolus formation and chromosome congression J. Cell Sci., June 15, 2007; 120(12): 2091 - 2105. [Abstract] [Full Text] [PDF]

				K. Hizume, S. Araki, K. Yoshikawa, and K. Takeyasu Topoisomerase II, scaffold component, promotes chromatin compaction in vitro in a linker-histone H1-dependent manner Nucleic Acids Res., April 11, 2007; (2007) gkm116v1. [Abstract] [Full Text] [PDF]

				H. Takata, S. Uchiyama, N. Nakamura, S. Nakashima, S. Kobayashi, T. Sone, S. Kimura, S. Lahmers, H. Granzier, S. Labeit, et al. A comparative proteome analysis of human metaphase chromosomes isolated from two different cell lines reveals a set of conserved chromosome-associated proteins Genes Cells, March 1, 2007; 12(3): 269 - 284. [Abstract] [Full Text] [PDF]

				M. Shinohara, A. V. Mikhailov, J. A. Aguirre-Ghiso, and C. L. Rieder Extracellular Signal-regulated Kinase 1/2 Activity Is Not Required in Mammalian Cells during Late G2 for Timely Entry into or Exit from Mitosis Mol. Biol. Cell, December 1, 2006; 17(12): 5227 - 5240. [Abstract] [Full Text] [PDF]

				A. Nishiyama, A. Dey, J.-i. Miyazaki, and K. Ozato Brd4 Is Required for Recovery from Antimicrotubule Drug-induced Mitotic Arrest: Preservation of Acetylated Chromatin Mol. Biol. Cell, February 1, 2006; 17(2): 814 - 823. [Abstract] [Full Text] [PDF]