|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 280, Issue 19, 18579-18589, May 13, 2005
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Interleukin-1
Decreases Expression of the Epithelial Sodium Channel 
-Subunit in Alveolar Epithelial Cells via a p38 MAPK-dependent Signaling Pathway*
¶||**¶||**¶||**||**¶||**¶¶
From the
Laboratory of Surgical Research, the **Cardiovascular Research Institute, the Lung Biology Center, and the Departments of Anesthesia, ¶Surgery, and ||Medicine, University of California, San Francisco, California 94110 and the Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520
Acute lung injury (ALI) is a devastating syndrome characterized by diffuse alveolar damage, elevated airspace levels of pro-inflammatory cytokines, and flooding of the alveolar spaces with protein-rich edema fluid. Interleukin-1
(IL-1) is one of the most biologically active cytokines in the distal airspaces of patients with ALI. IL-1 has been shown to increase lung epithelial and endothelial permeability. In this study, we hypothesized that IL-1 would decrease vectorial ion and water transport across the distal lung epithelium. Therefore, we measured the effects of IL-1 on transepithelial current, resistance, and sodium transport in primary cultures of alveolar epithelial type II (ATII) cells. IL-1 significantly reduced the amiloride-sensitive fraction of the transepithelial current and sodium transport across rat ATII cell monolayers. Moreover, IL-1 decreased basal and dexamethasone-induced epithelial sodium channel 
-subunit (ENaC) mRNA levels and total and cell-surface protein expression. The inhibitory effect of IL-1 on ENaC expression was mediated by the activation of p38 MAPK in both rat and human ATII cells and was independent of the activation of v6 integrin and transforming growth factor-. These results indicate that IL-1 may contribute to alveolar edema in ALI by reducing distal lung epithelial sodium absorption. This reduction in ion and water transport across the lung epithelium is in large part due to a decrease in ENaC expression through p38 MAPK-dependent inhibition of ENaC promoter activity and to an alteration in ENaC trafficking to the apical membrane of ATII cells.
Received for publication, September 14, 2004 , and in revised form, February 23, 2005.
* This work was supported by National Institutes of Health Grants R01GM62188 and P50HL074005 (to J.-F. P.) and Grant HL51854 (to M. A. M.) and by a fellowship grant from the Cystic Fibrosis Research, Inc. (to J. R.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The on-line version of this article (available at http://www.jbc.org) contains supplemental Fig. A.
¶¶ To whom correspondence should be addressed: Dept. of Anesthesia, San Francisco General Hospital, Rm. 3C-38, 1001 Potrero Ave., San Francisco, CA 94110. Tel.: 415-206-8163; Fax: 415-206-6014; E-mail: pittetj{at}anesthesia.ucsf.edu.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  F. Aldehni, M. Spitzner, J. R. Martins, R. Barro-Soria, R. Schreiber, and K. Kunzelmann Bestrophin 1 Promotes Epithelial-to-mesenchymal Transition of Renal Collecting Duct Cells J. Am. Soc. Nephrol., July 1, 2009; 20(7): 1556 - 1564. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. A. Bozza, A. M. Shah, A. S. Weyrich, and G. A. Zimmerman Amicus or Adversary: Platelets in Lung Biology, Acute Injury, and Inflammation Am. J. Respir. Cell Mol. Biol., February 1, 2009; 40(2): 123 - 134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. W. Chang, S. Hayashi, S. A. Gharib, T. Vaisar, S. T. King, M. Tsuchiya, J. T. Ruzinski, D. R. Park, G. Matute-Bello, M. M. Wurfel, et al. Proteomic and Computational Analysis of Bronchoalveolar Proteins during the Course of the Acute Respiratory Distress Syndrome Am. J. Respir. Crit. Care Med., October 1, 2008; 178(7): 701 - 709. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J A Frank, J Francois-Pittet, and M A Matthay Authors' reply Thorax, August 1, 2008; 63(8): 751 - 751. [Full Text] [PDF]
|
|
|
|
 |
|
 K. Namekata, C. Harada, K. Kohyama, Y. Matsumoto, and T. Harada Interleukin-1 Stimulates Glutamate Uptake in Glial Cells by Accelerating Membrane Trafficking of Na+/K+-ATPase via Actin Depolymerization Mol. Cell. Biol., May 15, 2008; 28(10): 3273 - 3280. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. T. Ganter, J. Roux, B. Miyazawa, M. Howard, J. A. Frank, G. Su, D. Sheppard, S. M. Violette, P. H. Weinreb, G. S. Horan, et al. Interleukin-1{beta} Causes Acute Lung Injury via {alpha}v{beta}5 and {alpha}v{beta}6 Integrin-Dependent Mechanisms Circ. Res., April 11, 2008; 102(7): 804 - 812. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. A. Drummond, S. C. Grifoni, and N. L. Jernigan A New Trick for an Old Dogma: ENaC Proteins as Mechanotransducers in Vascular Smooth Muscle Physiology, February 1, 2008; 23(1): 23 - 31. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. L. Wall and L. P. McCluskey Rapid Changes in Gustatory Function Induced by Contralateral Nerve Injury and Sodium Depletion Chem Senses, February 1, 2008; 33(2): 125 - 135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J A Frank, J-F Pittet, C Wray, and M A Matthay Protection from experimental ventilator-induced acute lung injury by IL-1 receptor blockade Thorax, February 1, 2008; 63(2): 147 - 153. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. S. Abayasiriwardana, D. Barbone, K.-U. Kim, C. Vivo, K. K. Lee, T. B. Dansen, A. E. Hunt, G. I. Evan, and V. C. Broaddus Malignant mesothelioma cells are rapidly sensitized to TRAIL-induced apoptosis by low-dose anisomycin via Bim Mol. Cancer Ther., October 1, 2007; 6(10): 2766 - 2776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Konopka, J. Tekiela, M. Iverson, C. Wells, and S. A. Duncan Junctional Adhesion Molecule-A Is Critical for the Formation of Pseudocanaliculi and Modulates E-cadherin Expression in Hepatic Cells J. Biol. Chem., September 21, 2007; 282(38): 28137 - 28148. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Lee, X. Fang, G. Dolganov, R. D. Fremont, J. A. Bastarache, L. B. Ware, and M. A. Matthay Acute Lung Injury Edema Fluid Decreases Net Fluid Transport across Human Alveolar Epithelial Type II Cells J. Biol. Chem., August 17, 2007; 282(33): 24109 - 24119. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Wang, J. A. Bastarache, N. Wickersham, X. Fang, M. A. Matthay, and L. B. Ware Novel Role of the Human Alveolar Epithelium in Regulating Intra-Alveolar Coagulation Am. J. Respir. Cell Mol. Biol., April 1, 2007; 36(4): 497 - 503. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Matthay and H. G. Folkesson Reduction of Alveolar Epithelial Ion and Fluid Transport by Inflammatory Mediators Am. J. Respir. Cell Mol. Biol., March 1, 2007; 36(3): 389 - 389. [Full Text] [PDF]
|
|
|
|
 |
|
 T. Li, S. Varadarajulu, L. L. Beard, J. Yun, and H. G. Folkesson A Noninflammatory Interleukin-1beta Fragment Stimulates Fetal Lung Fluid Absorption in Guinea Pigs J. Pharmacol. Exp. Ther., February 1, 2007; 320(2): 877 - 884. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Dagenais, R. Frechette, M.-E. Clermont, C. Masse, A. Prive, E. Brochiero, and Y. Berthiaume Dexamethasone inhibits the action of TNF on ENaC expression and activity Am J Physiol Lung Cell Mol Physiol, December 1, 2006; 291(6): L1220 - L1231. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. Grifoni, K. P. Gannon, D. E. Stec, and H. A. Drummond ENaC proteins contribute to VSMC migration Am J Physiol Heart Circ Physiol, December 1, 2006; 291(6): H3076 - H3086. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Jin, N. Kolliputi, D. Gou, T. Weng, and L. Liu A Novel Function of Ionotropic {gamma}-Aminobutyric Acid Receptors Involving Alveolar Fluid Homeostasis J. Biol. Chem., November 24, 2006; 281(47): 36012 - 36020. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Babilonia, D. Li, Z. Wang, P. Sun, D.-H. Lin, Y. Jin, and W.-H. Wang Mitogen-Activated Protein Kinases Inhibit the ROMK (Kir 1.1)-Like Small Conductance K Channels in the Cortical Collecting Duct J. Am. Soc. Nephrol., October 1, 2006; 17(10): 2687 - 2696. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Ganter, L. B. Ware, M. Howard, J. Roux, B. Gartland, M. A. Matthay, M. Fleshner, and J.-F. Pittet Extracellular heat shock protein 72 is a marker of the stress protein response in acute lung injury Am J Physiol Lung Cell Mol Physiol, September 1, 2006; 291(3): L354 - L361. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. G. Folkesson and M. A. Matthay Alveolar Epithelial Ion and Fluid Transport: Recent Progress Am. J. Respir. Cell Mol. Biol., July 1, 2006; 35(1): 10 - 19. [Full Text] [PDF]
|
|
|
|
|
|
T. Li and H. G. Folkesson RNA interference for {alpha}-ENaC inhibits rat lung fluid absorption in vivo Am J Physiol Lung Cell Mol Physiol, April 1, 2006; 290(4): L649 - L660. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Liu, T. M. Yang, W. Liedtke, and S. A. Simon Chronic IL-1beta Signaling Potentiates Voltage-Dependent Sodium Currents in Trigeminal Nociceptive Neurons J Neurophysiol, March 1, 2006; 95(3): 1478 - 1490. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Wesselkamper, L. M. Case, L. N. Henning, M. T. Borchers, J. W. Tichelaar, J. M. Mason, N. Dragin, M. Medvedovic, M. A. Sartor, C. R. Tomlinson, et al. Gene Expression Changes during the Development of Acute Lung Injury Role of Transforming Growth Factor {beta} Am. J. Respir. Crit. Care Med., December 1, 2005; 172(11): 1399 - 1411. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |