HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 2, 1230-1235, January 14, 2005

This Article Full Text Full Text (PDF) All Versions of this Article: 280/2/1230    most recent M408339200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Young, R. M. Articles by Baird, B. Search for Related Content PubMed PubMed Citation Articles by Young, R. M. Articles by Baird, B. Social Bookmarking                      What's this?

Reconstitution of Regulated Phosphorylation of FcRI by a Lipid Raft-excluded Protein-tyrosine Phosphatase^*

Ryan M. Young, Xinmin Zheng, David Holowka, and Barbara Baird¶

From the Department of Chemistry and Chemical Biology and the Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853

To examine the exquisite regulation of IgE-FcRI tyrosine phosphorylation by Lyn kinase that is stimulated by antigen-mediated cross-linking, we utilized co-expression of FcRI and Lyn in Chinese hamster ovary cells, which results in high basal levels of Lyn kinase activity and spontaneous phosphorylation of FcRI. We found that co-expression of a lipid raft-excluded transmembrane tyrosine phosphatase, PTP, suppresses Lyn kinase activity and markedly reduces the level of spontaneous phosphorylation of FcRI, while facilitating its antigen-stimulated phosphorylation. Other tyrosine phosphatases, including SHP-1, CD45, and a lipid raft-preferring chimeric version of PTP fail to reconstitute antigen-dependent FcRI phosphorylation. We concluded that both substrate specificity and submembrane location are critical to phosphatase-mediated regulation of Lyn kinase activity that supports activation of FcRI.

Received for publication, July 22, 2004 , and in revised form, October 22, 2004.

^* This work was supported by Grants R01-AI22449 and T32-GM07273 from the National Institutes of Health (to R. M. Y.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence should be addressed: Dept. of Chemistry and Chemical Biology, Cornell University, Baker Laboratory, Ithaca, NY 14853. Tel.: 607-255-4095; Fax: 607-255-4137; E-mail: bab13{at}cornell.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				R. M. Young, I. R. Hardy, R. L. Clarke, N. Lundy, P. Pine, B. C. Turner, T. A. Potter, and Y. Refaeli Mouse models of non-Hodgkin lymphoma reveal Syk as an important therapeutic target Blood, March 12, 2009; 113(11): 2508 - 2516. [Abstract] [Full Text] [PDF]

				C. M. Johnson, G. R. Chichili, and W. Rodgers Compartmentalization of Phosphatidylinositol 4,5-Bisphosphate Signaling Evidenced Using Targeted Phosphatases J. Biol. Chem., October 31, 2008; 283(44): 29920 - 29928. [Abstract] [Full Text] [PDF]

				K. G.N. Suzuki, T. K. Fujiwara, M. Edidin, and A. Kusumi Dynamic recruitment of phospholipase C{gamma} at transiently immobilized GPI-anchored receptor clusters induces IP3-Ca2+ signaling: single-molecule tracking study 2 J. Cell Biol., May 21, 2007; 177(4): 731 - 742. [Abstract] [Full Text] [PDF]

				D. A. Brown Lipid Rafts, Detergent-Resistant Membranes, and Raft Targeting Signals. Physiology, December 1, 2006; 21(6): 430 - 439. [Abstract] [Full Text] [PDF]

				D. R. Larson, J. A. Gosse, D. A. Holowka, B. A. Baird, and W. W. Webb Temporally resolved interactions between antigen-stimulated IgE receptors and Lyn kinase on living cells J. Cell Biol., November 7, 2005; 171(3): 527 - 536. [Abstract] [Full Text] [PDF]

				J. A. Gosse, A. Wagenknecht-Wiesner, D. Holowka, and B. Baird Transmembrane Sequences Are Determinants of Immunoreceptor Signaling J. Immunol., August 15, 2005; 175(4): 2123 - 2131. [Abstract] [Full Text] [PDF]