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J. Biol. Chem., Vol. 280, Issue 2, 1230-1235, January 14, 2005

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Reconstitution of Regulated Phosphorylation of FcRI by a Lipid Raft-excluded Protein-tyrosine Phosphatase^*

Ryan M. Young, Xinmin Zheng, David Holowka, and Barbara Baird¶

From the Department of Chemistry and Chemical Biology and the Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853

To examine the exquisite regulation of IgE-FcRI tyrosine phosphorylation by Lyn kinase that is stimulated by antigen-mediated cross-linking, we utilized co-expression of FcRI and Lyn in Chinese hamster ovary cells, which results in high basal levels of Lyn kinase activity and spontaneous phosphorylation of FcRI. We found that co-expression of a lipid raft-excluded transmembrane tyrosine phosphatase, PTP, suppresses Lyn kinase activity and markedly reduces the level of spontaneous phosphorylation of FcRI, while facilitating its antigen-stimulated phosphorylation. Other tyrosine phosphatases, including SHP-1, CD45, and a lipid raft-preferring chimeric version of PTP fail to reconstitute antigen-dependent FcRI phosphorylation. We concluded that both substrate specificity and submembrane location are critical to phosphatase-mediated regulation of Lyn kinase activity that supports activation of FcRI.

Received for publication, July 22, 2004 , and in revised form, October 22, 2004.

^* This work was supported by Grants R01-AI22449 and T32-GM07273 from the National Institutes of Health (to R. M. Y.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence should be addressed: Dept. of Chemistry and Chemical Biology, Cornell University, Baker Laboratory, Ithaca, NY 14853. Tel.: 607-255-4095; Fax: 607-255-4137; E-mail: bab13{at}cornell.edu.

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