| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 280, Issue 2, 1448-1456, January 14, 2005
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the
Department of Agricultural Chemistry, Kinki University, Nakamachi 3327-204, Nara 631-8505, ¶Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Ikoma, Nara 630-0101, Laboratory of Molecular Microbiology, School of Agriculture, ||Nagoya University, Chikusa-ku, Nagoya 464-8601, and **Division of Molecular Biology, Nippon Institute for Biological Science, Ome, Tokyo 198-0024, Japan
Bacteria possess a signal transduction system, referred to as a two-component system, for adaptation to external stimuli. Each two-component system consists of a sensor protein-histidine kinase (HK) and a response regulator (RR), together forming a signal transduction pathway via histidyl-aspartyl phospho-relay. A total of 30 sensor HKs, including as yet uncharacterized putative HKs (BaeS, BasS, CreC, CusS, HydH, RstB, YedV, and YfhK), and a total of 34 RRs, including putative RRs (BaeR, BasR, CreB, CusR, HydG, RstA, YedW, YfhA, YgeK, and YhjB), have been suggested to exist in Escherichia coli. We have purified the carboxyl-terminal catalytic domain of 27 sensor HKs and the full-length protein of all 34 RRs to apparent homogeneity. Self-phosphorylation in vitro was detected for 25 HKs. The rate of self-phosphorylation differed among HKs, whereas the level of phosphorylation was generally co-related with the phosphorylation rate. However, the phosphorylation level was low for ArcB, HydH, NarQ, and NtrB even though the reaction rate was fast, whereas the level was high for the slow phosphorylation species BasS, CheA, and CreC. By using the phosphorylated HKs, we examined trans-phosphorylation in vitro of RRs for all possible combinations. Trans-phosphorylation of presumed cognate RRs by HKs was detected, for the first time, for eight pairs, BaeS-BaeR, BasS-BasR, CreC-CreB, CusS-CusR, HydH-HydG, RstB-RstA, YedV-YedW, and YfhK-YfhA. All trans-phosphorylation took place within less than 1/2 min, but the stability of phosphorylated RRs differed, indicating the involvement of de-phosphorylation control. In addition to the trans-phosphorylation between the cognate pairs, we detected trans-phosphorylation between about 3% of non-cognate HK-RR pairs, raising the possibility that the cross-talk in signal transduction takes place between two-component systems.
Received for publication, September 2, 2004 , and in revised form, November 1, 2004.
* This work was supported by grants-in-aid for scientific research from the Ministry of Education, Science, Sports, and Culture of Japan and Research Grants from Kinki University and the Agricultural Chemical Research Foundation. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The on-line version of this article (available at http://www.jbc.org) contains Tables I and II.
To whom correspondence should be addressed. Tel.: 81-742-43-7274; Fax: 81-742-43-1445; E-mail: kyamam{at}nara.kindai.ac.jp.
This article has been cited by other articles:
|
|
 |
|
  A. Belcheva and D. Golemi-Kotra A Close-up View of the VraSR Two-component System: A MEDIATOR OF STAPHYLOCOCCUS AUREUS RESPONSE TO CELL WALL DAMAGE J. Biol. Chem., May 2, 2008; 283(18): 12354 - 12364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-L. Hsu, H.-C. Chen, H.-L. Peng, and H.-Y. Chang Characterization of the Histidine-containing Phosphotransfer Protein B-mediated Multistep Phosphorelay System in Pseudomonas aeruginosa PAO1 J. Biol. Chem., April 11, 2008; 283(15): 9933 - 9944. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Wolfe, N. Parikh, B. P. Lima, and B. Zemaitaitis Signal Integration by the Two-Component Signal Transduction Response Regulator CpxR J. Bacteriol., April 1, 2008; 190(7): 2314 - 2322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hamann, P. Zimmann, and K. Altendorf Reduction of Turgor Is Not the Stimulus for the Sensor Kinase KdpD of Escherichia coli J. Bacteriol., April 1, 2008; 190(7): 2360 - 2367. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Partridge, D. F. Browning, M. Xu, L. J. Newnham, C. Scott, R. E. Roberts, R. K. Poole, and J. Green Characterization of the Escherichia coli K-12 ydhYVWXUT operon: regulation by FNR, NarL and NarP Microbiology, February 1, 2008; 154(2): 608 - 618. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-L. Tzeng, C. M. Kahler, X. Zhang, and D. S. Stephens MisR/MisS Two-Component Regulon in Neisseria meningitidis Infect. Immun., February 1, 2008; 76(2): 704 - 716. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Eguchi, J. Itou, M. Yamane, R. Demizu, F. Yamato, A. Okada, H. Mori, A. Kato, and R. Utsumi B1500, a small membrane protein, connects the two-component systems EvgS/EvgA and PhoQ/PhoP in Escherichia coli PNAS, November 20, 2007; 104(47): 18712 - 18717. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Cabeza, A. Aguirre, F. C. Soncini, and E. G. Vescovi Induction of RpoS Degradation by the Two-Component System Regulator RstA in Salmonella enterica J. Bacteriol., October 15, 2007; 189(20): 7335 - 7342. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Matta, E. E. Lioliou, C. H. Panagiotidis, D. A. Kyriakidis, and C. A. Panagiotidis Interactions of the Antizyme AtoC with Regulatory Elements of the Escherichia coli atoDAEB Operon J. Bacteriol., September 1, 2007; 189(17): 6324 - 6332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Hussa, T. M. O'Shea, C. L. Darnell, E. G. Ruby, and K. L. Visick Two-Component Response Regulators of Vibrio fischeri: Identification, Mutagenesis, and Characterization J. Bacteriol., August 15, 2007; 189(16): 5825 - 5838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Carlsson, J. Liu, P. J. Edqvist, and M. S. Francis Extracytoplasmic-Stress-Responsive Pathways Modulate Type III Secretion in Yersinia pseudotuberculosis Infect. Immun., August 1, 2007; 75(8): 3913 - 3924. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ogasawara, A. Hasegawa, E. Kanda, T. Miki, K. Yamamoto, and A. Ishihama Genomic SELEX Search for Target Promoters under the Control of the PhoQP-RstBA Signal Relay Cascade J. Bacteriol., July 1, 2007; 189(13): 4791 - 4799. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Moker, J. Kramer, G. Unden, R. Kramer, and S. Morbach In Vitro Analysis of the Two-Component System MtrB-MtrA from Corynebacterium glutamicum J. Bacteriol., May 1, 2007; 189(9): 3645 - 3649. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Bibb, C. A. Kunkle, and M. P. Schmitt The ChrA-ChrS and HrrA-HrrS Signal Transduction Systems Are Required for Activation of the hmuO Promoter and Repression of the hemA Promoter in Corynebacterium diphtheriae Infect. Immun., May 1, 2007; 75(5): 2421 - 2431. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. L. Campbell, C. S. Ederer, A. Allali-Hassani, and E. D. Brown Isolation of the rstA Gene as a Multicopy Suppressor of YjeE, an Essential ATPase of Unknown Function in Escherichia coli J. Bacteriol., April 15, 2007; 189(8): 3318 - 3321. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Wu, F. Mao, V. Olman, and Y. Xu Hierarchical classification of functionally equivalent genes in prokaryotes Nucleic Acids Res., April 1, 2007; 35(7): 2125 - 2140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Fleischer, R. Heermann, K. Jung, and S. Hunke Purification, Reconstitution, and Characterization of the CpxRAP Envelope Stress System of Escherichia coli J. Biol. Chem., March 23, 2007; 282(12): 8583 - 8593. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Ma and J.-R. Zhang RR06 Activates Transcription of spr1996 and cbpA in Streptococcus pneumoniae J. Bacteriol., March 15, 2007; 189(6): 2497 - 2509. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. C. Reading, A. G. Torres, M. M. Kendall, D. T. Hughes, K. Yamamoto, and V. Sperandio A Novel Two-Component Signaling System That Activates Transcription of an Enterohemorrhagic Escherichia coli Effector Involved in Remodeling of Host Actin J. Bacteriol., March 15, 2007; 189(6): 2468 - 2476. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. A. Golubeva and J. M. Slauch Salmonella enterica Serovar Typhimurium Periplasmic Superoxide Dismutase SodCI Is a Member of the PhoPQ Regulon and Is Induced in Macrophages J. Bacteriol., November 15, 2006; 188(22): 7853 - 7861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.-K. Cho, E. M. Knight, and B. O. Palsson Transcriptional regulation of the fad regulon genes of Escherichia coli by ArcA. Microbiology, August 1, 2006; 152(Pt 8): 2207 - 2219. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. Kusters, A. H. M. van Vliet, and E. J. Kuipers Pathogenesis of Helicobacter pylori Infection Clin. Microbiol. Rev., July 1, 2006; 19(3): 449 - 490. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Venkatesh, L. Babujee, H. Liu, P. Hedley, T. Fujikawa, P. Birch, I. Toth, and S. Tsuyumu The Erwinia chrysanthemi 3937 PhoQ Sensor Kinase Regulates Several Virulence Determinants. J. Bacteriol., April 1, 2006; 188(8): 3088 - 3098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Nadler, Y. Shifrin, S. Nov, S. Kobi, and I. Rosenshine Characterization of Enteropathogenic Escherichia coli Mutants That Fail To Disrupt Host Cell Spreading and Attachment to Substratum Infect. Immun., February 1, 2006; 74(2): 839 - 849. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Mika and R. Hengge A two-component phosphotransfer network involving ArcB, ArcA, and RssB coordinates synthesis and proteolysis of {sigma}S (RpoS) in E. coli Genes & Dev., November 15, 2005; 19(22): 2770 - 2781. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
