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J. Biol. Chem., Vol. 280, Issue 2, 1594-1602, January 14, 2005

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Analysis of Transport Activity of Arabidopsis Sugar Alcohol Permease Homolog AtPLT5^*

Anke Reinders, Jody A. Panshyshyn, and John M. Ward

From the Department of Plant Biology, University of Minnesota Twin Cities, St. Paul, Minnesota 55108

The transporters responsible for sugar uptake into non-photosynthetic sink tissues in plants, such as roots and flowers, have not been fully identified and analyzed. Plants encode around 100 putative sugar transporters within the major facilitator superfamily, yet only a few have been studied. Here we report the analysis of a sugar alcohol permease homolog (AtPLT5, At3g18830) from Arabidopsis. A wide range of sugars including hexoses, pentoses, tetroses, a sugar acid, and sugar alcohols but not disaccharides induced inward currents in oocytes expressing AtPLT5. AtPLT5 expression also resulted in ¹⁴C-labeled substrate uptake in oocytes, indicating that AtPLT5 encodes an ion-coupled uptake transporter. K_0.5 values for glucose and sorbitol were highly dependent on external pH. Expression of AtPLT5 was found primarily in sink tissues: in the elongation zone of roots, in the inflorescence stem, and several floral structures, especially in the floral abscission zone. Expression was induced by mechanical wounding and insect feeding. Analysis of transport properties and expression in Arabidopsis indicate that AtPLT5 functions to transport a wide range of sugars into specific sink tissues in the plant.

Received for publication, September 21, 2004 , and in revised form, November 2, 2004.

^* This work was supported by United States Department of Agriculture Grant 2002-35304-12481 and United States Department of Energy Grant DE-FG02-03ER15414. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Plant Biology Dept., 250 Biological Sciences Center, 1445 Gortner Ave., St. Paul, MN 55108. Tel.: 612-625-4763; Fax: 612-625-1738; E-mail:: jward{at}tc.umn.edu.

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