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J. Biol. Chem., Vol. 280, Issue 20, 19576-19586, May 20, 2005

This Article Full Text Full Text (PDF) All Versions of this Article: 280/20/19576    most recent M502513200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Martins, A. H. B. Articles by Ulrich, H. Search for Related Content PubMed PubMed Citation Articles by Martins, A. H. B. Articles by Ulrich, H. Social Bookmarking                      What's this?

Neuronal Differentiation of P19 Embryonal Carcinoma Cells Modulates Kinin B2 Receptor Gene Expression and Function^*

Antonio Henrique B. Martins, Rodrigo R. Resende, Paromita Majumder, Marcella Faria, Dulce E. Casarini¶, Attila Tárnok||, Walter Colli, João Bosco Pesquero, and Henning Ulrich, Supported by Fundação de Amparo à Pesquisa do Estado de São Paulo, Project No. 2001/08827-4, Conselho Nacional de Desenvolvimento Científico, and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil**

From the Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo 05508-900, Brazil, the Departamento de Biofísica, Universidade Federal de São Paulo, São Paulo 04023-062, Brazil, the ^¶Departamento de Nefrologia, Universidade Federal de São Paulo, São Paulo 04044-020, Brazil, and ^||Pediatric Cardiology, Cardiac Center Leipzig, University of Leipzig, 04280 Leipzig, Germany

Kinins are vasoactive oligopeptides generated upon proteolytic cleavage of low and high molecular weight kininogens by kallikreins. These peptides have a well established signaling role in inflammation and homeostasis. Nevertheless, emerging evidence suggests that bradykinin and other kinins are stored in the central nervous system and may act as neuromediators in the control of nociceptive response. Here we show that the kinin-B2 receptor (B2BKR) is differentially expressed during in vitro neuronal differentiation of P19 cells. Following induction by retinoic acid, cells form embryonic bodies and then undergo neuronal differentiation, which is complete after 8 and 9 days. Immunochemical staining revealed that B2BKR protein expression was below detection limits in nondifferentiated P19 cells but increased during the course of neuronal differentiation and peaked on days 8 and 9. Measurement of [Ca²⁺]_i in the absence and presence of bradykinin showed that most undifferentiated cells are unresponsive to bradykinin application, but following differentiation, P19 cells express high molecular weight neurofilaments, secrete bradykinin into the culture medium, and respond to bradykinin application with a transient increase in [Ca²⁺]_i. However, inhibition of B2BKR activity with HOE-140 during early differentiation led to a decrease in the size of embryonic bodies formed. Pretreatment of differentiating P19 cells with HOE-140 on day 5 resulted in a reduction of the calcium response induced by the cholinergic agonist carbamoylcholine and decreased expression levels of M1–M3 muscarinic acetylcholine receptors, indicating crucial functions of the B2BKR during neuronal differentiation.

Received for publication, March 7, 2005

^* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^** To whom correspondence should be addressed: Dept. de Bioquímica, Instituto de Química, Universidade de São Paulo, Caixa Postal 26077, CEP 05513-970, São Paulo, Brazil. Tel.: 55-11-3091-3810 (ext. 223); Fax: 55-11-3815-5579; E-mail: henning{at}iq.usp.br.

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