HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 21, 20185-20188, May 27, 2005

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 280/21/20185    most recent C500044200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Brand, L. A. Articles by Strauss, E. Search for Related Content PubMed PubMed Citation Articles by Brand, L. A. Articles by Strauss, E. Social Bookmarking                      What's this?

Characterization of a New Pantothenate Kinase Isoform from Helicobacter pylori^*

Leisl A. Brand and Erick Strauss

From the Department of Chemistry, Stellenbosch University, Matieland 7602, South Africa

Pantothenate kinase (PanK) catalyzes the first step in the biosynthesis of the essential and ubiquitous cofactor coenzyme A (CoA) in all organisms. Two well characterized isoforms of the enzyme are known: a prokaryotic PanK that predominates in eubacteria and a eukaryotic isoform that has primarily been characterized from mammalian and plant sources. Curiously, the genomes of certain pathogenic bacteria, including Helicobacter pylori and Pseudomonas aeruginosa, do not contain a PanK similar to either isoform, although these organisms possess all the other biosynthetic machinery required for CoA production. In this study we cloned, overexpressed and characterized an enzyme from Bacillus subtilis and its homologue from H. pylori and show that they catalyze the ATP-dependent phosphorylation of pantothenate. These enzymes do not share sequence homology with any known PanK, and unlike the bacterial and eukaryotic PanK isoforms their activity is not regulated by either CoA or acetyl-CoA. They also do not accept the pantothenic acid antimetabolite N-pentylpantothenamide as a substrate or are inhibited by it. Taken together, these results point to the identification of a third distinct isoform of PanK that accounts for the only known activity of the enzyme in pathogens such as H. pylori and P. aeruginosa.

Received for publication, January 31, 2005 , and in revised form, March 9, 2005.

^* This work was supported by grants from the National Research Foundation of South Africa (GUN 2054218) and from Stellenbosch University. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Fig. 1 and supplemental Table I.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank^TM/EBI Data Bank with accession number(s) AY912104.

To whom correspondence should be addressed: Dept. of Chemistry, Private Bag X1, Stellenbosch University, Matieland 7602, South Africa. Tel.: 27-21-808-3355; Fax: 27-21-808-3360; E-mail: estrauss{at}sun.ac.za.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. Paige, S. D. Reid, P. C. Hanna, and A. Claiborne The Type III Pantothenate Kinase Encoded by coaX Is Essential for Growth of Bacillus anthracis J. Bacteriol., September 15, 2008; 190(18): 6271 - 6275. [Abstract] [Full Text] [PDF]

				A. M. Lehane, R. V. Marchetti, C. Spry, D. A. van Schalkwyk, R. Teng, K. Kirk, and K. J. Saliba Feedback Inhibition of Pantothenate Kinase Regulates Pantothenol Uptake by the Malaria Parasite J. Biol. Chem., August 31, 2007; 282(35): 25395 - 25405. [Abstract] [Full Text] [PDF]

				K. Yang, Y. Eyobo, L. A. Brand, D. Martynowski, D. Tomchick, E. Strauss, and H. Zhang Crystal Structure of a Type III Pantothenate Kinase: Insight into the Mechanism of an Essential Coenzyme A Biosynthetic Enzyme Universally Distributed in Bacteria. J. Bacteriol., August 1, 2006; 188(15): 5532 - 5540. [Abstract] [Full Text] [PDF]

				Y.-M. Zhang, S. W. White, and C. O. Rock Inhibiting Bacterial Fatty Acid Synthesis J. Biol. Chem., June 30, 2006; 281(26): 17541 - 17544. [Abstract] [Full Text] [PDF]

				R. Overbeek, T. Begley, R. M. Butler, J. V. Choudhuri, H.-Y. Chuang, M. Cohoon, V. de Crecy-Lagard, N. Diaz, T. Disz, R. Edwards, et al. The Subsystems Approach to Genome Annotation and its Use in the Project to Annotate 1000 Genomes Nucleic Acids Res., October 7, 2005; 33(17): 5691 - 5702. [Abstract] [Full Text] [PDF]