JBC Oz Biosciences

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M500334200 on March 21, 2005

J. Biol. Chem., Vol. 280, Issue 21, 20894-20901, May 27, 2005
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
280/21/20894    most recent
M500334200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yin, Q. Y.
Right arrow Articles by de Koster, C. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yin, Q. Y.
Right arrow Articles by de Koster, C. G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Comprehensive Proteomic Analysis of Saccharomyces cerevisiae Cell Walls

IDENTIFICATION OF PROTEINS COVALENTLY ATTACHED VIA GLYCOSYLPHOSPHATIDYLINOSITOL REMNANTS OR MILD ALKALI-SENSITIVE LINKAGES*{boxs}

Qing Yuan Yin{ddagger}, Piet W. J. de Groot{ddagger}§, Henk L. Dekker{ddagger}, Luitzen de Jong{ddagger}, Frans M. Klis¶, and Chris G. de Koster{ddagger}

From the {ddagger}Laboratory for Mass Spectrometry of Biomacromolecules and Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands

The cell wall of yeast contains proteins that are covalently bound to the glycan network. These cell wall proteins (CWPs) mediate cell-cell interactions and may be involved in cell wall biosynthesis. Using tandem mass spectrometry, we have identified 19 covalently bound CWPs of Saccharomyces cerevisiae. Twelve of them are shown for the first time to be covalently incorporated into the cell wall. The identified proteins include 12 predicted glycosylphosphatidylinositol-modified CWPs, all four members of the Pir protein family, and three additional proteins (Scw4p, Scw10p, and Tos1p) that are, like Pir proteins, connected to the cell wall glycan network via an alkali-sensitive linkage. However, Scw4p, Scw10p, and Tos1p do not contain internal repeat sequences shown to be essential for Pir protein incorporation and may represent a separate class of CWPs. Strikingly, seven of the identified proteins (Gas1p, Gas3p, Gas5p, Crh1p, Utr2p, Scw4p, and Scw10p) are classified as glycoside hydrolases. Phenotypic analysis of deletion mutants lacking the corresponding CWP-encoding genes indicated that most of them have altered cell wall properties, which reinforces the importance of the identified proteins for proper cell wall formation. In particular, gas1{Delta} and ecm33{Delta} were highly sensitive to Calcofluor White and high temperature, whereas gas1{Delta}, scw4{Delta}, and tos1{Delta} were highly resistant to incubation with {beta}-1,3-glucanase. The CWP identification method developed here relies on directly generating tryptic peptides from isolated cell walls and is independent of the nature of the covalent linkages between CWPs and cell wall glycans. Therefore, it will probably be equally effective in many other fungi.

Received for publication, January 10, 2005 , and in revised form, February 28, 2005.

* This work was supported by the European Commission (QLK2-2000-00795; "Galar Fungail Consortium"). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

{boxs} The on-line version of this article (available at http://www.jbc.org) contains Supplemental Table I.

§ To whom correspondence should be addressed: Swammerdam Institute for Life Sciences, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands. Tel.: 31-20-525-7053; Fax: 31-20-525-7056; E-mail: pgroot{at}science.uva.nl.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Microbiol. Mol. Biol. Rev.Home page
W. L. Chaffin
Candida albicans Cell Wall Proteins
Microbiol. Mol. Biol. Rev., September 1, 2008; 72(3): 495 - 544.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Popolo, E. Ragni, C. Carotti, O. Palomares, R. Aardema, J. W. Back, H. L. Dekker, L. J. de Koning, L. de Jong, and C. G. de Koster
Disulfide Bond Structure and Domain Organization of Yeast {beta}(1,3)-Glucanosyltransferases Involved in Cell Wall Biogenesis
J. Biol. Chem., July 4, 2008; 283(27): 18553 - 18565.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
K. Matsui, S. Teranishi, S. Kamon, K. Kuroda, and M. Ueda
Discovery of a Modified Transcription Factor Endowing Yeasts with Organic-Solvent Tolerance and Reconstruction of an Organic-Solvent-Tolerant Saccharomyces cerevisiae Strain
Appl. Envir. Microbiol., July 1, 2008; 74(13): 4222 - 4225.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
G. J. Sosinska, P. W. J. de Groot, M. J. Teixeira de Mattos, H. L. Dekker, C. G. de Koster, K. J. Hellingwerf, and F. M. Klis
Hypoxic conditions and iron restriction affect the cell-wall proteome of Candida albicans grown under vagina-simulative conditions
Microbiology, February 1, 2008; 154(2): 510 - 520.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
J. E. Coronado, S. Mneimneh, S. L. Epstein, W.-G. Qiu, and P. N. Lipke
Conserved Processes and Lineage-Specific Proteins in Fungal Cell Wall Evolution
Eukaryot. Cell, December 1, 2007; 6(12): 2269 - 2277.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
P. Orlean and A. K. Menon
Thematic review series: Lipid Posttranslational Modifications. GPI anchoring of protein in yeast and mammalian cells, or: how we learned to stop worrying and love glycophospholipids
J. Lipid Res., May 1, 2007; 48(5): 993 - 1011.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
J. Nett, L. Lincoln, K. Marchillo, R. Massey, K. Holoyda, B. Hoff, M. VanHandel, and D. Andes
Putative Role of {beta}-1,3 Glucans in Candida albicans Biofilm Resistance
Antimicrob. Agents Chemother., February 1, 2007; 51(2): 510 - 520.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
E. Ragni, A. Coluccio, E. Rolli, J. M. Rodriguez-Pena, G. Colasante, J. Arroyo, A. M. Neiman, and L. Popolo
GAS2 and GAS4, a Pair of Developmentally Regulated Genes Required for Spore Wall Assembly in Saccharomyces cerevisiae
Eukaryot. Cell, February 1, 2007; 6(2): 302 - 316.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Pardini, P. W. J. De Groot, A. T. Coste, M. Karababa, F. M. Klis, C. G. de Koster, and D. Sanglard
The CRH Family Coding for Cell Wall Glycosylphosphatidylinositol Proteins with a Predicted Transglycosidase Domain Affects Cell Wall Organization and Virulence of Candida albicans
J. Biol. Chem., December 29, 2006; 281(52): 40399 - 40411.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. S. Bickel and D. R. Morris
Role of the Transcription Activator Ste12p as a Repressor of PRY3 Expression
Mol. Cell. Biol., November 1, 2006; 26(21): 7901 - 7912.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
G. J. Smits, L. R. Schenkman, S. Brul, J. R. Pringle, and F. M. Klis
Role of Cell Cycle-regulated Expression in the Localized Incorporation of Cell Wall Proteins in Yeast
Mol. Biol. Cell, July 1, 2006; 17(7): 3267 - 3280.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
G. Lesage and H. Bussey
Cell Wall Assembly in Saccharomyces cerevisiae
Microbiol. Mol. Biol. Rev., June 1, 2006; 70(2): 317 - 343.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
R. Bosson, M. Jaquenoud, and A. Conzelmann
GUP1 of Saccharomyces cerevisiae Encodes an O-Acyltransferase Involved in Remodeling of the GPI Anchor
Mol. Biol. Cell, June 1, 2006; 17(6): 2636 - 2645.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
K. L. Van Dellen, A. Chatterjee, D. M. Ratner, P. E. Magnelli, J. F. Cipollo, M. Steffen, P. W. Robbins, and J. Samuelson
Unique Posttranslational Modifications of Chitin-Binding Lectins of Entamoeba invadens Cyst Walls
Eukaryot. Cell, May 1, 2006; 5(5): 836 - 848.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Ecker, R. Deutzmann, L. Lehle, V. Mrsa, and W. Tanner
Pir Proteins of Saccharomyces cerevisiae Are Attached to beta-1,3-Glucan by a New Protein-Carbohydrate Linkage
J. Biol. Chem., April 28, 2006; 281(17): 11523 - 11529.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
S. M. Bowman, A. Piwowar, M. Al Dabbous, J. Vierula, and S. J. Free
Mutational Analysis of the Glycosylphosphatidylinositol (GPI) Anchor Pathway Demonstrates that GPI-Anchored Proteins Are Required for Cell Wall Biogenesis and Normal Hyphal Growth in Neurospora crassa
Eukaryot. Cell, March 1, 2006; 5(3): 587 - 600.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
M. G. Slattery, D. Liko, and W. Heideman
The Function and Properties of the Azf1 Transcriptional Regulator Change with Growth Conditions in Saccharomyces cerevisiae
Eukaryot. Cell, February 1, 2006; 5(2): 313 - 320.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
E. J. Tarcha, V. Basrur, C.-Y. Hung, M. J. Gardner, and G. T. Cole
A Recombinant Aspartyl Protease of Coccidioides posadasii Induces Protection against Pulmonary Coccidioidomycosis in Mice
Infect. Immun., January 1, 2006; 74(1): 516 - 527.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
J. Singh, D. Kumar, N. Ramakrishnan, V. Singhal, J. Jervis, J. F. Garst, S. M. Slaughter, A. M. DeSantis, M. Potts, and R. F. Helm
Transcriptional Response of Saccharomyces cerevisiae to Desiccation and Rehydration
Appl. Envir. Microbiol., December 1, 2005; 71(12): 8752 - 8763.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.