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J. Biol. Chem., Vol. 280, Issue 22, 21376-21383, June 3, 2005

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A Specific Interaction between Muskelin and the Cyclin-dependent Kinase 5 Activator p39 Promotes Peripheral Localization of Muskelin^*

Dolena R. Ledee, Chun Y. Gao, Ranjana Seth, Robert N. Fariss, Brajendra K. Tripathi, and Peggy S. Zelenka

From the NEI, National Institutes of Health, Bethesda, Maryland 20892-0704

Previous studies implicate cyclin-dependent kinase 5 in cell adhesion and migration of epithelial cells of the cornea and lens. To explore molecular interactions underlying these functions, we performed yeast two-hybrid screening of an embryonic rat lens library for proteins that interact with cyclin-dependent kinase 5 and its regulators, p35 and p39. This screen identified a specific interaction between p39 and muskelin, an intracellular protein known to affect cytoskeletal organization in adherent cells. Immunohistochemistry detected muskelin in the developing lens and in other tissues, including brain and muscle. Glutathione S-transferase pull-down experiments and co-immunoprecipitations confirmed the specificity of the p39-muskelin interaction. Deletion analysis of p39 showed that muskelin binds to the p39 C terminus, which contains a short insertion (amino acids 329–366) absent from p35. Similar analysis of muskelin mapped the interaction with p39 to the fifth kelch repeat. Co-expression of p39 and muskelin in COS1 cells or lens epithelial cells altered the intracellular localization of muskelin, recruiting it to the cell periphery. These findings demonstrate a novel interaction between muskelin and the cyclin-dependent kinase 5 activator p39 and suggest that p39 may regulate the subcellular localization of muskelin.

Received for publication, February 2, 2005 , and in revised form, March 24, 2005.

^* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Present address: Bascom Palmer Eye Inst., University of Miami School of Medicine, 1638 N. W. 10th Ave., Miami, FL 33136.

To whom correspondence should be addressed: National Institutes of Health, Bldg. 7, Rm. 102, 7 Memorial Dr., MSC 0704, Bethesda, MD 20892-0704. Tel.: 301-496-7490; Fax: 301-435-7682; E-mail: zelenkap{at}nei.nih.gov.

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