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J. Biol. Chem., Vol. 280, Issue 24, 23057-23065, June 17, 2005

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Histone H1 Proteins Act As Receptors for the 987P Fimbriae of Enterotoxigenic Escherichia coli^*

Guoqiang Zhu, Huaiqing Chen, Byung-Kwon Choi, Fabio Del Piero, and Dieter M. Schifferli¶

From the Department of Pathobiology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, Pennsylvania, 19104

The tip adhesin FasG of the 987P fimbriae of enterotoxigenic Escherichia coli mediates two distinct adhesive interactions with brush border molecules of the intestinal epithelial cells of neonatal piglets. First, FasG attaches strongly to sulfatide with hydroxylated fatty acyl chains. This interaction involves lysine 117 and other lysine residues of FasG. Second, FasG recognizes specific intestinal brush border proteins that migrate on a sodium-dodecyl sulfate-polyacrylamide gel like a distinct set of 32–35-kDa proteins, as shown by ligand blotting assays. The protein sequence of high performance liquid chromatography-purified tryptic fragments of the major protein band matched sequences of human and murine histone H1 proteins. Porcine histone H1 proteins isolated from piglet intestinal epithelial cells demonstrated the same SDS-PAGE migration pattern and 987P binding properties as the 987P-specific protein receptors from porcine intestinal brush borders. Binding was dose-dependent and shown to be specific in adhesion inhibition and gel migration shift assays. Moreover, mapping of the histone H1 binding domain suggested that it is located in their lysine-rich C-terminal domains. Histone H1 molecules were visualized on the microvilli of intestinal epithelial cells by immunohistochemistry and electron microscopy. Taken together these results indicated that the intestinal protein receptors for 987P are histone H1 proteins. It is suggested that histones are released into the intestinal lumen by the high turnover of the intestinal epithelium. Their strong cationic properties can explain their association with the negatively charged brush border surfaces. There, the histone H1 molecules stabilize the sulfatide-fimbriae interaction by simultaneously binding to the membrane and to 987P.

Received for publication, April 4, 2005

^* This work was supported by the United States Department of Agriculture, National Research Initiative Grant 2002-35204-12216. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Present address: University of Yangzhou School of Veterinary Medicine, Yangzhou, Jiangsu 225009, China.

Present address: GlycoFi, Inc., 21 Lafayette St. Suite 200, Lebanon, NH 03766.

^¶ To whom correspondence should be addressed: University of Pennsylvania School of Veterinary Medicine, 3800 Spruce St., Philadelphia, PA 19104-6049. Tel.: 215-898-1685; Fax: 215-898-7887; E-mail: dmschiff{at}vet.upenn.edu.

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