HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 26, 24591-24599, July 1, 2005

This Article Full Text Full Text (PDF) All Versions of this Article: 280/26/24591    most recent M501872200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Klotzsche, M. Articles by Hillen, W. Search for Related Content PubMed PubMed Citation Articles by Klotzsche, M. Articles by Hillen, W. Social Bookmarking                      What's this?

A Peptide Triggers Allostery in Tet Repressor by Binding to a Unique Site^*

Marcus Klotzsche, Christian Berens, and Wolfgang Hillen

From the Lehrstuhl für Mikrobiologie, Institut für Mikrobiologie, Biochemie und Genetik, Friedrich-Alexander Universität Erlangen-Nürnberg, Staudtstrasse 5, 91058 Erlangen, Germany

Regulatory proteins often communicate with each other to manage various cellular processes. Such interactions mostly rely on the recognition of small peptide motifs. The activity of other regulatory proteins depends on small molecular weight effectors and allostery. We demonstrate the in vivo regulation of the tetracycline-dependent Tet repressor by an oligopeptide fused to the N or C terminus of thioredoxin A. The binding site of the peptide overlaps but is not identical with the tetracycline binding site. Several TetR mutants that are non-inducible by tetracycline also respond to the peptide. This demonstrates for the first time the conversion of a small molecular weight effector-dependent regulator to a protein-protein contact-dependent potential member of designed signaling chains.

Received for publication, February 18, 2005 , and in revised form, May 2, 2005.

^* This work was supported by the Deutsche Forschungsgemeinschaft through the SFB 473, the Graduierten Kolleg 805, and the Fonds der Chemischen Industrie. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed. Tel.: 49-9131-8528081; Fax: 49-9131-8528082; E-mail: whillen{at}biologie.uni-erlangen.de.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Klotzsche, D. Goeke, C. Berens, and W. Hillen Efficient and exclusive induction of Tet repressor by the oligopeptide Tip results from co-variation of their interaction site Nucleic Acids Res., June 9, 2007; 35(12): 3945 - 3952. [Abstract] [Full Text] [PDF]

				M. Schlicht, C. Berens, J. Daam, and W. Hillen Random Insertion of a TetR-Inducing Peptide Tag into Escherichia coli Proteins Allows Analysis of Protein Levels by Induction of Reporter Gene Expression Appl. Envir. Microbiol., August 1, 2006; 72(8): 5637 - 5642. [Abstract] [Full Text] [PDF]