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Originally published In Press as doi:10.1074/jbc.M500625200 on May 3, 2005 Originally published In Press as doi:10.1074/jbc.M500625200 on April 27, 2005 Originally published In Press as doi:10.1074/jbc.M500625200 on April 22, 2005

J. Biol. Chem., Vol. 280, Issue 26, 24706-24714, July 1, 2005
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Transcriptional Profiles of the Human Pathogenic Fungus Paracoccidioides brasiliensis in Mycelium and Yeast Cells*{boxs}

Maria Sueli S. Felipe,ab Rosângela V. Andrade,ac Fabrício B. M. Arraes,ac André M. Nicola,ac Andréa Q. Maranhão,a Fernando A. G. Torres,a Ildinete Silva-Pereira,a Márcio J. Poças-Fonseca,a Élida G. Campos,a Lídia M. P. Moraes,a Patrícia A. Andrade,a Aldo H. F. P. Tavares,a Simoneide S. Silva,a Cynthia M. Kyaw,a Diorge P. Souza,a PbGenome Network,d Maristela Pereira,a Rosália S. A. Jesuíno,e Edmar V. Andrade,e Juliana A. Parente,e Gisele S. Oliveira,e Mônica S. Barbosa,e Natália F. Martins,f Ana L. Fachin,g Renato S. Cardoso,g Geraldo A. S. Passos,gh Nalvo F. Almeida,i Maria Emília M. T. Walter,j Célia M. A. Soares,e Maria José A. Carvalho,ac and Marcelo M. Brígidoac

From the aDepartamento de Biologia Celular, Universidade de Brasília, 70910-900, Brasília, DF, Brazil, jDepartamento de Ciência da Computação, Universidade de Brasília, 70910-900, Brasília, DF, Brazil, fEmbrapa-Recursos Genéticos e Biotecnologia, W5 Norte, 70770-900, Brasília, DF, Brazil, eDepartamento de Bioquímica, Universidade Federal de Goiás, 74001-970, Goiânia, GO, Brazil, iDepartamento de Computação e Estatística, Universidade Federal de Mato Grosso do Sul, 79070-900, Campo Grande, Mississippi, Brazil, gDepartamento de Genética, Universidade de São Paulo, 14040-900, Ribeirão Preto, SP, Brazil, and hFaculdade de Odontologia, Universidade de São Paulo, 14040-900, Ribeirão Preto, SP, Brazil

Paracoccidioides brasiliensis is the causative agent of paracoccidioidomycosis, a disease that affects 10 million individuals in Latin America. This report depicts the results of the analysis of 6,022 assembled groups from mycelium and yeast phase expressed sequence tags, covering about 80% of the estimated genome of this dimorphic, thermo-regulated fungus. The data provide a comprehensive view of the fungal metabolism, including overexpressed transcripts, stage-specific genes, and also those that are up- or down-regulated as assessed by in silico electronic subtraction and cDNA microarrays. Also, a significant differential expression pattern in mycelium and yeast cells was detected, which was confirmed by Northern blot analysis, providing insights into differential metabolic adaptations. The overall transcriptome analysis provided information about sequences related to the cell cycle, stress response, drug resistance, and signal transduction pathways of the pathogen. Novel P. brasiliensis genes have been identified, probably corresponding to proteins that should be addressed as virulence factor candidates and potential new drug targets.

Received for publication, January 18, 2005 , and in revised form, March 15, 2005.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) CA580326-CA584263, CN238087-CN253933, and CN373644-CN373755.

Minimal information about cDNA microarray experiments was deposited in the MIAMExpress databank (EMBL) under the accession numbers E-MEXP-103 and A-MEXP-71. The sequences are also available at https://www.biomol.unb.br/Pb.

* This work was supported by MCT, CNPq, CAPES, FUB, UFG, and FUNDECT-MS.

{boxs} The on-line version of this article (available at http://www.jbc.org) contains nine additional tables.

c These authors contributed equally to this work.

d PbGenome Network: Alda Maria T. Ferreira, Alessandra Dantas, Alessandra J. Baptista, Alexandre M. Bailão, Ana Lídia Bonato, André C. Amaral, Bruno S. Daher, Camila M. Silva, Christiane S. Costa, Clayton L. Borges, Cléber O. Soares, Cristina M. Junta, Daniel A. S. Anjos, Edans F. O. Sandes, Eduardo A. Donadi, Elza T. Sakamoto-Hojo, Flábio R. Araújo, Flávia C. Albuquerque, Gina C. Oliveira, João Ricardo M. Almeida, Juliana C. Oliveira, Kláudia G. Jorge, Larissa Fernandes, Lorena S. Derengowski, Luís Artur M. Bataus, Marcus A. M. Araújo, Marcus K. Inoue, Marlene T. De-Souza, Mauro F. Almeida, Nádia S. Parachin, Nadya S. Castro, Odair P. Martins, Patrícia L. N. Costa, Paula Sandrin-Garcia, Renata B. A. Soares, Stephano S. Mello, and Viviane C. B. Reis.

b To whom correspondence should be addressed. Tel.: 55-307-2423; Fax: 55-61-3498411; E-mail: msueli{at}unb.br.


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