JBC INTERFERin siRNA transfection reagent

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M500625200 on May 3, 2005 Originally published In Press as doi:10.1074/jbc.M500625200 on April 27, 2005 Originally published In Press as doi:10.1074/jbc.M500625200 on April 22, 2005

J. Biol. Chem., Vol. 280, Issue 26, 24706-24714, July 1, 2005
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
280/26/24706    most recent
M500625200v3
M500625200v2
M500625200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Felipe, M. S. S.
Right arrow Articles by Brígido, M. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Felipe, M. S. S.
Right arrow Articles by Brígido, M. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Transcriptional Profiles of the Human Pathogenic Fungus Paracoccidioides brasiliensis in Mycelium and Yeast Cells*{boxs}

Maria Sueli S. Felipe,ab Rosângela V. Andrade,ac Fabrício B. M. Arraes,ac André M. Nicola,ac Andréa Q. Maranhão,a Fernando A. G. Torres,a Ildinete Silva-Pereira,a Márcio J. Poças-Fonseca,a Élida G. Campos,a Lídia M. P. Moraes,a Patrícia A. Andrade,a Aldo H. F. P. Tavares,a Simoneide S. Silva,a Cynthia M. Kyaw,a Diorge P. Souza,a PbGenome Network,d Maristela Pereira,a Rosália S. A. Jesuíno,e Edmar V. Andrade,e Juliana A. Parente,e Gisele S. Oliveira,e Mônica S. Barbosa,e Natália F. Martins,f Ana L. Fachin,g Renato S. Cardoso,g Geraldo A. S. Passos,gh Nalvo F. Almeida,i Maria Emília M. T. Walter,j Célia M. A. Soares,e Maria José A. Carvalho,ac and Marcelo M. Brígidoac

From the aDepartamento de Biologia Celular, Universidade de Brasília, 70910-900, Brasília, DF, Brazil, jDepartamento de Ciência da Computação, Universidade de Brasília, 70910-900, Brasília, DF, Brazil, fEmbrapa-Recursos Genéticos e Biotecnologia, W5 Norte, 70770-900, Brasília, DF, Brazil, eDepartamento de Bioquímica, Universidade Federal de Goiás, 74001-970, Goiânia, GO, Brazil, iDepartamento de Computação e Estatística, Universidade Federal de Mato Grosso do Sul, 79070-900, Campo Grande, Mississippi, Brazil, gDepartamento de Genética, Universidade de São Paulo, 14040-900, Ribeirão Preto, SP, Brazil, and hFaculdade de Odontologia, Universidade de São Paulo, 14040-900, Ribeirão Preto, SP, Brazil

Paracoccidioides brasiliensis is the causative agent of paracoccidioidomycosis, a disease that affects 10 million individuals in Latin America. This report depicts the results of the analysis of 6,022 assembled groups from mycelium and yeast phase expressed sequence tags, covering about 80% of the estimated genome of this dimorphic, thermo-regulated fungus. The data provide a comprehensive view of the fungal metabolism, including overexpressed transcripts, stage-specific genes, and also those that are up- or down-regulated as assessed by in silico electronic subtraction and cDNA microarrays. Also, a significant differential expression pattern in mycelium and yeast cells was detected, which was confirmed by Northern blot analysis, providing insights into differential metabolic adaptations. The overall transcriptome analysis provided information about sequences related to the cell cycle, stress response, drug resistance, and signal transduction pathways of the pathogen. Novel P. brasiliensis genes have been identified, probably corresponding to proteins that should be addressed as virulence factor candidates and potential new drug targets.

Received for publication, January 18, 2005 , and in revised form, March 15, 2005.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) CA580326-CA584263, CN238087-CN253933, and CN373644-CN373755.

Minimal information about cDNA microarray experiments was deposited in the MIAMExpress databank (EMBL) under the accession numbers E-MEXP-103 and A-MEXP-71. The sequences are also available at https://www.biomol.unb.br/Pb.

* This work was supported by MCT, CNPq, CAPES, FUB, UFG, and FUNDECT-MS.

{boxs} The on-line version of this article (available at http://www.jbc.org) contains nine additional tables.

c These authors contributed equally to this work.

d PbGenome Network: Alda Maria T. Ferreira, Alessandra Dantas, Alessandra J. Baptista, Alexandre M. Bailão, Ana Lídia Bonato, André C. Amaral, Bruno S. Daher, Camila M. Silva, Christiane S. Costa, Clayton L. Borges, Cléber O. Soares, Cristina M. Junta, Daniel A. S. Anjos, Edans F. O. Sandes, Eduardo A. Donadi, Elza T. Sakamoto-Hojo, Flábio R. Araújo, Flávia C. Albuquerque, Gina C. Oliveira, João Ricardo M. Almeida, Juliana C. Oliveira, Kláudia G. Jorge, Larissa Fernandes, Lorena S. Derengowski, Luís Artur M. Bataus, Marcus A. M. Araújo, Marcus K. Inoue, Marlene T. De-Souza, Mauro F. Almeida, Nádia S. Parachin, Nadya S. Castro, Odair P. Martins, Patrícia L. N. Costa, Paula Sandrin-Garcia, Renata B. A. Soares, Stephano S. Mello, and Viviane C. B. Reis.

b To whom correspondence should be addressed. Tel.: 55-307-2423; Fax: 55-61-3498411; E-mail: msueli{at}unb.br.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 MicrobiologyHome page
M. Costa, C. L. Borges, A. M. Bailao, G. V. Meirelles, Y. A. Mendonca, S. F. I. M. Dantas, F. P. de Faria, M. S. S. Felipe, E. E. W. I. Molinari-Madlum, M. J. S. Mendes-Giannini, et al.
Transcriptome profiling of Paracoccidioides brasiliensis yeast-phase cells recovered from infected mice brings new insights into fungal response upon host interaction
Microbiology, December 1, 2007; 153(12): 4194 - 4207.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
W. L. Batista, A. L. Matsuo, L. Ganiko, T. F. Barros, T. R. Veiga, E. Freymuller, and R. Puccia
The PbMDJ1 Gene Belongs to a Conserved MDJ1/LON Locus in Thermodimorphic Pathogenic Fungi and Encodes a Heat Shock Protein That Localizes to both the Mitochondria and Cell Wall of Paracoccidioides brasiliensis
Eukaryot. Cell, February 1, 2006; 5(2): 379 - 390.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
L. R. Nunes, R. Costa de Oliveira, D. B. Leite, V. S. da Silva, E. dos Reis Marques, M. E. da Silva Ferreira, D. C. D. Ribeiro, L. A. de Souza Bernardes, M. H. S. Goldman, R. Puccia, et al.
Transcriptome Analysis of Paracoccidioides brasiliensis Cells Undergoing Mycelium-to-Yeast Transition
Eukaryot. Cell, December 1, 2005; 4(12): 2115 - 2128.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.