HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 26, 25250-25257, July 1, 2005

This Article Full Text Full Text (PDF) All Versions of this Article: 280/26/25250    most recent M414222200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Daval, M. Articles by Foufelle, F. Search for Related Content PubMed PubMed Citation Articles by Daval, M. Articles by Foufelle, F. Social Bookmarking                      What's this?

Anti-lipolytic Action of AMP-activated Protein Kinase in Rodent Adipocytes^*

Marie Daval, Francine Diot-Dupuy, Raymond Bazin, Isabelle Hainault, Benoît Viollet¶, Sophie Vaulont¶, Eric Hajduch, Pascal Ferré, and Fabienne Foufelle||

From the INSERM U671, Centre de Recherches Biomédicales des Cordeliers, Université Pierre et Marie Curie, 75270 Paris Cedex 06, France and ^¶Institut Cochin, Département de Génétique, Développement et Pathologie Moléculaire, Faculté de Médecine Cochin-Port Royal, 75014 Paris, France

Despite its importance in terms of energy homeostasis, the role of AMP-activated protein kinase in adipose tissue remains controversial. Initial studies have described an anti-lipolytic role for AMP-activated protein kinase, whereas more recent studies have suggested the converse. Thus we have addressed the role of AMP-activated protein kinase in adipose tissue by modulating AMP-activated protein kinase activity in primary rodent adipocytes using pharmacological activators or by adenoviral expression of dominant negative or constitutively active forms of the kinase. We then studied the effects of AMP-activated protein kinase activity modulation on lipolytic mechanisms. Finally, we analyzed the consequences of a genetic deletion of AMP-activated protein kinase in mouse adipocytes. AMP-activated protein kinase activity in adipocytes is represented mainly by the ₁ isoform and is induced by all of the stimuli that increase cAMP in adipocytes, including fasting. When AMP-activated protein kinase activity is increased by 5-aminoimidazole-4-carboxamide-riboside, phenformin, or by the expression of a constitutively active form, isoproterenol-induced lipolysis is strongly reduced. Conversely, when AMP-activated protein kinase activity is decreased either by a dominant negative form or in AMP-activated protein kinase ₁ knock-out mice, lipolysis is increased. We present data suggesting that AMP-activated protein kinase acts on hormone-sensitive lipase by blocking its translocation to the lipid droplet. We conclude that, in mature adipocytes, AMP-activated protein kinase activation has a clear anti-lipolytic effect.

Received for publication, December 17, 2004 , and in revised form, March 22, 2005.

^* This work is supported by a grant from Alfediam-Takeda and by two contracts (QLG1-CT-2001-01488, Ampdiamet and LSHM-CT-2004-005272, Exgenesis) from the European commission. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Recipient of a doctoral fellowship from the Ministère de l'Enseignement Supérieur et de la Recherche, France.

^|| To whom correspondence should be addressed: U671 INSERM, Centre de Recherches Biomédicales des Cordeliers, 15, rue de l'Ecole de Médecine, 75270 Paris Cedex 06, France. Tel.: 33-1-42-34-69-23; Fax: 33-1-40-51-85-86; E-mail: foufelle{at}bhdc.jussieu.fr.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K. Choi, E. Mollapour, J. H. Choi, and S. B. Shears Cellular Energetic Status Supervises the Synthesis of Bis-Diphosphoinositol Tetrakisphosphate Independently of AMP-Activated Protein Kinase Mol. Pharmacol., August 1, 2008; 74(2): 527 - 536. [Abstract] [Full Text] [PDF]

				M.-S. Gauthier, H. Miyoshi, S. C. Souza, J. M. Cacicedo, A. K. Saha, A. S. Greenberg, and N. B. Ruderman AMP-activated Protein Kinase Is Activated as a Consequence of Lipolysis in the Adipocyte: POTENTIAL MECHANISM AND PHYSIOLOGICAL RELEVANCE J. Biol. Chem., June 13, 2008; 283(24): 16514 - 16524. [Abstract] [Full Text] [PDF]

				M. Lu, Q. Tang, J. M. Olefsky, P. L. Mellon, and N. J. G. Webster Adiponectin Activates Adenosine Monophosphate-Activated Protein Kinase and Decreases Luteinizing Hormone Secretion in L{beta}T2 Gonadotropes Mol. Endocrinol., March 1, 2008; 22(3): 760 - 771. [Abstract] [Full Text] [PDF]

				S.-J. Kim, C. Nian, and C. H. S. McIntosh Activation of Lipoprotein Lipase by Glucose-dependent Insulinotropic Polypeptide in Adipocytes: A ROLE FOR A PROTEIN KINASE B, LKB1, AND AMP-ACTIVATED PROTEIN KINASE CASCADE J. Biol. Chem., March 23, 2007; 282(12): 8557 - 8567. [Abstract] [Full Text] [PDF]

				B. Luo, G. J. Parker, R. C. Cooksey, Y. Soesanto, M. Evans, D. Jones, and D. A. McClain Chronic Hexosamine Flux Stimulates Fatty Acid Oxidation by Activating AMP-activated Protein Kinase in Adipocytes J. Biol. Chem., March 9, 2007; 282(10): 7172 - 7180. [Abstract] [Full Text] [PDF]

				M. C. Towler and D. G. Hardie AMP-Activated Protein Kinase in Metabolic Control and Insulin Signaling Circ. Res., February 16, 2007; 100(3): 328 - 341. [Abstract] [Full Text] [PDF]

				M. P. Gaidhu, S. Fediuc, and R. B. Ceddia 5-Aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside-induced AMP-activated Protein Kinase Phosphorylation Inhibits Basal and Insulin-stimulated Glucose Uptake, Lipid Synthesis, and Fatty Acid Oxidation in Isolated Rat Adipocytes J. Biol. Chem., September 8, 2006; 281(36): 25956 - 25964. [Abstract] [Full Text] [PDF]

				M. Daval, F. Foufelle, and P. Ferre Functions of AMP-activated protein kinase in adipose tissue J. Physiol., July 1, 2006; 574(1): 55 - 62. [Abstract] [Full Text] [PDF]

				D. S. Hutchinson and T. Bengtsson AMP-Activated Protein Kinase Activation by Adrenoceptors in L6 Skeletal Muscle Cells: Mediation by {alpha}1-Adrenoceptors Causing Glucose Uptake Diabetes, March 1, 2006; 55(3): 682 - 690. [Abstract] [Full Text] [PDF]