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J. Biol. Chem., Vol. 280, Issue 27, 25323-25330, July 8, 2005

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Carbon Source-dependent Assembly of the Snf1p Kinase Complex in Candida albicans^*

Carsten Corvey¶, Peter Koetter||**, Tobias Beckhaus, Jeremy Hack, Sandra Hofmann, Martin Hampel, Torsten Stein||, Michael Karas, and Karl-Dieter Entian||

From the Institutes for ^||Microbiology and Pharmaceutical Chemistry, Johann Wolfgang Goethe-University of Frankfurt, Marie-Curie-Strasse 9, D-60439 Frankfurt, Germany

The Snf1p/AMP-activated kinases are involved in transcriptional, metabolic, and developmental regulation in response to stress. In Saccharomyces cerevisiae, Snf1p (Cat1p) is one of the key regulators of carbohydrate metabolism, and cat1 (snf1) mutants fail to grow with non-fermentable carbon sources. In Candida albicans, Snf1p is an essential protein and cells depend on a functional Snf1 kinase even with glucose as carbon source. We investigated the CaSnf1p complex after tandem affinity purification and mass spectrometric analysis and show that the complex composition changes with the carbon source provided. Three subunits were identified, one of which was named CaSnf4p because of its homology to the ScSnf4 protein and the respective CaSNF4 gene could complement a S. cerevisiae snf4 mutant. The other two proteins revealed similarities to the S. cerevisiae kinase subunits ScGal83p, ScSip2p, and ScSip1p. Both genes complemented the scaffold function in a S. cerevisiae gal83,sip1,sip2 triple deletion mutant and were named according to their scaffold function as CaKIS1p and CaKIS2p. Matrix-assisted laser desorption ionization peptide mass fingerprint analysis indicated that CaKis2p is N-terminal myristoylated and the incorporation of CaKis2p in the Snf1p complex was reduced when compared with cells grown with glucose as a carbon source. To verify the different complex assemblies, a stable isotope labeling technique (iTraq^TM) was employed, confirming a 3-fold decrease of CaKis2p with ethanol. Yeast two-hybrid analysis confirmed the interaction partners, and these results showed an activator domain for the CaKis2 protein that has not been reported for S. cerevisiae scaffold subunits.

Received for publication, April 5, 2005 , and in revised form, May 4, 2005.

^* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains two supplemental tables.

Both authors contributed equally to this work.

^¶ To whom correspondence may be addressed. Tel.: 49-69-798-29925; Fax: 49-69-798-29918; E-mail: corvey{at}iachem.uni-frankfurt.de. ** To whom correspondence may be addressed. Tel.: 49-69-798-29529; Fax: 49-69-798-29527; E-mail: koetter{at}em.uni-frankfurt.de.

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