HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 27, 25580-25589, July 8, 2005

This Article Full Text Full Text (PDF) All Versions of this Article: 280/27/25580    most recent M504103200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chiu, P. C. N. Articles by Yeung, W. S.B. Search for Related Content PubMed PubMed Citation Articles by Chiu, P. C. N. Articles by Yeung, W. S.B. Social Bookmarking                      What's this?

Glycodelin-S in Human Seminal Plasma Reduces Cholesterol Efflux and Inhibits Capacitation of Spermatozoa^*

Philip C. N. Chiu, Man-Kin Chung, Heng-Yan Tsang, Riitta Koistinen¶, Hannu Koistinen¶, Markku Seppala¶, Kai-Fai Lee, and William S.B. Yeung||

From the Department of Obstetrics and Gynaecology, University of Hong Kong, Queen Mary Hospital, Pokfulam Road, Hong Kong, China and the Departments of Obstetrics and Gynaecology and ^¶Clinical Chemistry, University Central Hospital, 00029 HUS Helsinki, Finland

Tight control of sperm capacitation is important for successful fertilization. Glycodelin-S is one of the most abundant glycoproteins in the human seminal plasma. However, its function is unclear. We investigated the role of glycodelin-S on capacitation of human spermatozoa. Binding kinetics experiments demonstrated the presence of two saturable and reversible binding sites of glycodelin-S on human spermatozoa. Differently glycosylated other isoforms of glycodelin, glycodelin-A and -F, did not compete with glycodelin-S for these binding sites, suggesting that the glycodelin-S binding sites are different from those of the other isoforms. Indirect immunofluorescent staining revealed specific binding of glycodelin-S around the sperm head. This immunoreactivity was greatly reduced in spermatozoa that had migrated through the cervical mucus surrogates. Glycodelin-S at physiological concentrations significantly reduced the bovine serum albumin and cyclodextrin-induced cholesterol efflux and down-regulated the adenylyl cyclase/protein kinase A/tyrosine kinase signaling pathway, resulting in suppression of capacitation. Deglycosylation abolished glycodelin-S binding and the effect of glycodelin-S on bovine serum albumin-induced capacitation. This indicates that the carbohydrate moiety of glycodelin-S is critical for the function of the molecule. It is concluded that glycodelin-S in seminal plasma maintains the uncapacitated state of human spermatozoa.

Received for publication, April 15, 2005 , and in revised form, May 9, 2005.

^* This work was supported by the Research Grant Council, Hong Kong (Grants HKU7261/01M and HKU7614/05M), Committee on Research and Conference Grants, University of Hong Kong, Helsinki University Central Hospital Research Funds, Federation of the Finnish Life and Pension Insurance Companies, the Cancer Society of Finland, the Academy of Finland, and the University of Helsinki.The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^|| To whom correspondence should be addressed. Tel.: 852-285-53405; Fax: 852-281-75374; E-mail: wsbyeung{at}hkucc.hku.hk.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				P. C.N. Chiu, B. S.T. Wong, M.-K. Chung, K. K.W. Lam, R. T.K. Pang, K.-F. Lee, S.B. Sumitro, S.K. Gupta, and W. S.B. Yeung Effects of Native Human Zona Pellucida Glycoproteins 3 and 4 on Acrosome Reaction and Zona Pellucida Binding of Human Spermatozoa Biol Reprod, November 1, 2008; 79(5): 869 - 877. [Abstract] [Full Text] [PDF]

				P. C.N. Chiu, B. S.T. Wong, C.L. Lee, R. T.K. Pang, K.-F. Lee, S.B. Sumitro, S.K. Gupta, and W. S.B. Yeung Native human zona pellucida glycoproteins: purification and binding properties Hum. Reprod., June 1, 2008; 23(6): 1385 - 1393. [Abstract] [Full Text] [PDF]

				J. Girouard, G. Frenette, and R. Sullivan Seminal Plasma Proteins Regulate the Association of Lipids and Proteins Within Detergent-Resistant Membrane Domains of Bovine Spermatozoa Biol Reprod, May 1, 2008; 78(5): 921 - 931. [Abstract] [Full Text] [PDF]

				M. K. Tee, J.-L. Vigne, J. Yu, and R. N. Taylor Natural and recombinant human glycodelin activate a proapoptotic gene cascade in monocyte cells J. Leukoc. Biol., April 1, 2008; 83(4): 843 - 852. [Abstract] [Full Text] [PDF]

				R. Oren-Benaroya, J. Kipnis, and M. Eisenbach Phagocytosis of human post-capacitated spermatozoa by macrophages Hum. Reprod., November 1, 2007; 22(11): 2947 - 2955. [Abstract] [Full Text] [PDF]

				H. Uchida, T. Maruyama, K. Ohta, M. Ono, T. Arase, M. Kagami, H. Oda, T. Kajitani, H. Asada, and Y. Yoshimura Histone deacetylase inhibitor-induced glycodelin enhances the initial step of implantation Hum. Reprod., October 1, 2007; 22(10): 2615 - 2622. [Abstract] [Full Text] [PDF]

				M. Seppala, H. Koistinen, R. Koistinen, P.C.N. Chiu, and W.S.B. Yeung Glycosylation related actions of glycodelin: gamete, cumulus cell, immune cell and clinical associations Hum. Reprod. Update, May 1, 2007; 13(3): 275 - 287. [Abstract] [Full Text] [PDF]

				P. C. N. Chiu, M.-K. Chung, R. Koistinen, H. Koistinen, M. Seppala, P.-C. Ho, E. H. Y. Ng, K.-F. Lee, and W. S. B. Yeung Cumulus Oophorus-associated Glycodelin-C Displaces Sperm-bound Glycodelin-A and -F and Stimulates Spermatozoa-Zona Pellucida Binding J. Biol. Chem., February 23, 2007; 282(8): 5378 - 5388. [Abstract] [Full Text] [PDF]

				P. C. N. Chiu, M.-K. Chung, R. Koistinen, H. Koistinen, M. Seppala, P.-C. Ho, E. H. Y. Ng, K.-F. Lee, and W. S. B. Yeung Glycodelin-A interacts with fucosyltransferase on human sperm plasma membrane to inhibit spermatozoa-zona pellucida binding J. Cell Sci., January 1, 2007; 120(1): 33 - 44. [Abstract] [Full Text] [PDF]

				K. Lapid and N. Sharon Meet the multifunctional and sexy glycoforms of glycodelin Glycobiology, March 1, 2006; 16(3): 39R - 45R. [Abstract] [Full Text] [PDF]