HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 27, 25907-25912, July 8, 2005

This Article Full Text Full Text (PDF) All Versions of this Article: 280/27/25907    most recent M412476200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Saurí, A. Articles by Mingarro, I. Search for Related Content PubMed PubMed Citation Articles by Saurí, A. Articles by Mingarro, I. Social Bookmarking                      What's this?

Double-spanning Plant Viral Movement Protein Integration into the Endoplasmic Reticulum Membrane Is Signal Recognition Particle-dependent, Translocon-mediated, and Concerted^*

Ana Saurí, Suraj Saksena¶, Jesús Salgado, Arthur E. Johnson¶||**, and Ismael Mingarro

From the Departament de Bioquímica i Biologia Molecular, Universitat de València E-46 100 Burjassot, Spain, the Departments of ^¶Biochemistry and Biophysics and ^||Chemistry, Texas A&M University, College Station, Texas 77843, and the ^**Department of Medical Biochemistry and Genetics, Texas A&M University System Health Science Center, College Station, Texas 77843-1114

The current model for cell-to-cell movement of plant viruses holds that transport requires virus-encoded movement proteins that intimately associate with endoplasmic reticulum membranes. We have examined the early stages of the integration into endoplasmic reticulum membranes of a double-spanning viral movement protein using photocross-linking. We have discovered that this process is cotranslational and proceeds in a signal recognition particle-dependent manner. In addition, nascent chain photocross-linking to Sec61 and translocating chain-associated membrane protein reveal that viral membrane protein insertion takes place via the translocon, as with most eukaryotic membrane proteins, but that the two transmembrane segments of the viral protein leave the translocon and enter the lipid bilayer together.

Received for publication, November 4, 2004 , and in revised form, May 9, 2005.

^* This work was supported by Grants BMC2003-01532 from the Spanish MCyT and GV04B-183 from the Generalitat Valenciana (to I. M.) and by National Institutes of Health Grant R01 GM26494 and by the Robert A. Welch Foundation (to A. E. J.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

A recipient of a predoctoral fellowship from the Spanish Ministerio de Educación y Ciencia.

To whom correspondence should be addressed. Tel.: 34-96-354-3796; Fax: 34-96-354-4635; E-mail: Ismael.Mingarro{at}uv.es.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				L. Martinez-Gil, J. A. Sanchez-Navarro, A. Cruz, V. Pallas, J. Perez-Gil, and I. Mingarro Plant Virus Cell-to-Cell Movement Is Not Dependent on the Transmembrane Disposition of Its Movement Protein J. Virol., June 1, 2009; 83(11): 5535 - 5543. [Abstract] [Full Text] [PDF]

				B. C. S. Cross and S. High Dissecting the physiological role of selective transmembrane-segment retention at the ER translocon J. Cell Sci., June 1, 2009; 122(11): 1768 - 1777. [Abstract] [Full Text] [PDF]

				D. Pitonzo, Z. Yang, Y. Matsumura, A. E. Johnson, and W. R. Skach Sequence-specific Retention and Regulated Integration of a Nascent Membrane Protein by the Endoplasmic Reticulum Sec61 Translocon Mol. Biol. Cell, January 1, 2009; 20(2): 685 - 698. [Abstract] [Full Text] [PDF]

				M. Fujiki, S. Kawakami, R. W. Kim, and R. N. Beachy Domains of tobacco mosaic virus movement protein essential for its membrane association J. Gen. Virol., September 1, 2006; 87(9): 2699 - 2707. [Abstract] [Full Text] [PDF]

				N. Ismail, S. G. Crawshaw, and S. High Active and passive displacement of transmembrane domains both occur during opsin biogenesis at the Sec61 translocon J. Cell Sci., July 1, 2006; 119(13): 2826 - 2836. [Abstract] [Full Text] [PDF]

				S. Deitermann, G. S. Sprie, and H.-G. Koch A Dual Function for SecA in the Assembly of Single Spanning Membrane Proteins in Escherichia coli J. Biol. Chem., November 25, 2005; 280(47): 39077 - 39085. [Abstract] [Full Text] [PDF]