HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 28, 25994-26001, July 15, 2005

This Article Full Text Full Text (PDF) All Versions of this Article: 280/28/25994    most recent M500508200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pineau, B. Articles by Chétrit, P. Search for Related Content PubMed PubMed Citation Articles by Pineau, B. Articles by Chétrit, P. Social Bookmarking                      What's this?

Targeting the NAD7 Subunit to Mitochondria Restores a Functional Complex I and a Wild Type Phenotype in the Nicotiana sylvestris CMS II Mutant Lacking nad7^*

Bernard Pineau, Chantal Mathieu, Catherine Gérard-Hirne, Rosine De Paepe, and Philippe Chétrit

From the Institut de Biotechnologie des Plantes, Laboratoire Mitochondries et Métabolisme Centre National de la Recherche Scientifique-Université Paris-Sud, Unite Mixte de Recherche 8618, 91405 Orsay, France

The mitochondrial DNA of the Nicotiana sylvestris CMSII mutant carries a 72-kb deletion comprising the single copy nad7 gene that encodes the NAD7 subunit of the respiratory complex I (NADH-ubiquinone oxidoreductase). CMSII plants lack rotenone-sensitive complex I activity and are impaired in physiological and phenotypical traits. To check whether these changes directly result from the deletion of nad7, we constructed CMS transgenic plants (termed as CMSnad7) carrying an edited nad7 cDNA fused to the CAMV ³⁵S promoter and to a mitochondrial targeting sequence. The nad7 sequence was transcribed and translated and the NAD7 protein directed to mitochondria in CMSnad7 transgenic plants, which recovered both wild type morphology and growth features. Blue-native/SDS gel electrophoresis and enzymatic assays showed that, whereas fully assembled complex I was absent from CMSII mitochondria, a functional complex was present in CMSnad7 mitochondria. Furthermore, a supercomplex involving complex I and complex III was present in CMSnad7 as in the wild type. Taken together, these data demonstrate that lack of complex I in CMSII was indeed the direct consequence of the absence of nad7. Hence, NAD7 is a key element for complex assembly in plants. These results also show that allotopic expression from the nucleus can fully complement the lack of a mitochondrial-encoded complex I gene.

Received for publication, January 14, 2005 , and in revised form, April 7, 2005.

Addendum—A recent study supports the view that the protein complexes forming the mitochondrial respiratory chain are organized in respirasomes in spinach leaves (Krause, F., Reifschneider, N. H., Vocke, D., Seelert H., Rexroth, S., and Dencher, N. A. (2004) J. Biol. Chem. 279, 48369–48375).

^* This work was supported by CNRS and Université Paris-Sud, UMR8618. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed. Tel.: 33-1-69-15-34-07; Fax: 33-1-69-15-34-25; E-mail: chetrit{at}ibp.u-psud.fr.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				I. Marques, N. A. Dencher, A. Videira, and F. Krause Supramolecular Organization of the Respiratory Chain in Neurospora crassa Mitochondria Eukaryot. Cell, December 1, 2007; 6(12): 2391 - 2405. [Abstract] [Full Text] [PDF]

				A. F. de Longevialle, E. H. Meyer, C. Andres, N. L. Taylor, C. Lurin, A. H. Millar, and I. D. Small The Pentatricopeptide Repeat Gene OTP43 Is Required for trans-Splicing of the Mitochondrial nad1 Intron 1 in Arabidopsis thaliana PLANT CELL, October 1, 2007; 19(10): 3256 - 3265. [Abstract] [Full Text] [PDF]

				S. Pyndiah, J. P. Lasserre, A. Menard, S. Claverol, V. Prouzet-Mauleon, F. Megraud, F. Zerbib, and M. Bonneu Two-dimensional Blue Native/SDS Gel Electrophoresis of Multiprotein Complexes from Helicobacter pylori Mol. Cell. Proteomics, February 1, 2007; 6(2): 193 - 206. [Abstract] [Full Text] [PDF]

				S. M. Khan, R. M. Smigrodzki, and R. H. Swerdlow Cell and animal models of mtDNA biology: progress and prospects Am J Physiol Cell Physiol, February 1, 2007; 292(2): C658 - C669. [Abstract] [Full Text] [PDF]

				P. Cardol, M. Lapaille, P. Minet, F. Franck, R. F. Matagne, and C. Remacle ND3 and ND4L Subunits of Mitochondrial Complex I, Both Nucleus Encoded in Chlamydomonas reinhardtii, Are Required for Activity and Assembly of the Enzyme. Eukaryot. Cell, September 1, 2006; 5(9): 1460 - 1467. [Abstract] [Full Text] [PDF]

				P Priault, G Tcherkez, G Cornic, R De Paepe, R Naik, J Ghashghaie, and P Streb The lack of mitochondrial complex I in a CMSII mutant of Nicotiana sylvestris increases photorespiration through an increased internal resistance to CO2 diffusion J. Exp. Bot., September 1, 2006; 57(12): 3195 - 3207. [Abstract] [Full Text] [PDF]

				C. Dutilleul, C. Lelarge, J.-L. Prioul, R. De Paepe, C. H. Foyer, and G. Noctor Mitochondria-Driven Changes in Leaf NAD Status Exert a Crucial Influence on the Control of Nitrate Assimilation and the Integration of Carbon and Nitrogen Metabolism Plant Physiology, September 1, 2005; 139(1): 64 - 78. [Abstract] [Full Text] [PDF]